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Nat Commun. 2019 Apr 9;10(1):1629. doi: 10.1038/s41467-019-09549-4.
Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites.
Alenquer M1, Vale-Costa S1, Etibor TA1, Ferreira F1, Sousa AL1,2, Amorim MJ3.
Author information
Abstract
Influenza A virus has an eight-partite RNA genome that during viral assembly forms a complex containing one copy of each RNA. Genome assembly is a selective process driven by RNA-RNA interactions and is hypothesized to lead to discrete punctate structures scattered through the cytosol. Here, we show that contrary to the accepted view, formation of these structures precedes RNA-RNA interactions among distinct viral ribonucleoproteins (vRNPs), as they assemble in cells expressing only one vRNP type. We demonstrate that these viral inclusions display characteristics of liquid organelles, segregating from the cytosol without a delimitating membrane, dynamically exchanging material and adapting fast to environmental changes. We provide evidence that viral inclusions develop close to endoplasmic reticulum (ER) exit sites, depend on continuous ER-Golgi vesicular cycling and do not promote escape to interferon response. We propose that viral inclusions segregate vRNPs from the cytosol and facilitate selected RNA-RNA interactions in a liquid environment.
PMID: 30967547 PMCID: PMC6456594 DOI: 10.1038/s41467-019-09549-4
Free PMC Article
Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites.
Alenquer M1, Vale-Costa S1, Etibor TA1, Ferreira F1, Sousa AL1,2, Amorim MJ3.
Author information
Abstract
Influenza A virus has an eight-partite RNA genome that during viral assembly forms a complex containing one copy of each RNA. Genome assembly is a selective process driven by RNA-RNA interactions and is hypothesized to lead to discrete punctate structures scattered through the cytosol. Here, we show that contrary to the accepted view, formation of these structures precedes RNA-RNA interactions among distinct viral ribonucleoproteins (vRNPs), as they assemble in cells expressing only one vRNP type. We demonstrate that these viral inclusions display characteristics of liquid organelles, segregating from the cytosol without a delimitating membrane, dynamically exchanging material and adapting fast to environmental changes. We provide evidence that viral inclusions develop close to endoplasmic reticulum (ER) exit sites, depend on continuous ER-Golgi vesicular cycling and do not promote escape to interferon response. We propose that viral inclusions segregate vRNPs from the cytosol and facilitate selected RNA-RNA interactions in a liquid environment.
PMID: 30967547 PMCID: PMC6456594 DOI: 10.1038/s41467-019-09549-4
Free PMC Article