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Arch Virol. 2008 Sep 30. [Epub ahead of print]
Cold co-extraction of hemagglutinin and matrix M1 protein from influenza virus A by a combination of non-ionic detergents allows for visualization of the raft-like nature of the virus envelope.
Radyukhin V, Fedorova N, Ksenofontov A, Serebryakova M, Baratova L. - Department of Chromatography, A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Leninskie Gory, Moscow, 119992, Russia, varvic@belozersky.msu.ru.
Membrane solubilization with a mixture of cold non-ionic detergents has been applied to isolate detergent-resistant membranes from intact virus A lipid bilayer.
Association of the viral envelope glycoproteins and M1 into a raft lipid-protein complex was verified via detergent insolubility experiments, and the M1:HA stoichiometry of the proposed supramolecular complex was estimated via amino acid analysis.
Electron microscopy and dynamic light scattering data revealed that these lipid-protein rafts form unilamellar vesicles with HA spikes on their surfaces similar to influenza virus virions.
Together, our data suggest that the cold co-extraction technique visualizes the raft-like nature of the viral envelope and demonstrates the interaction of matrix M1 protein with the envelope.
PMID: 18825482 [PubMed - as supplied by publisher]
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Cold co-extraction of hemagglutinin and matrix M1 protein from influenza virus A by a combination of non-ionic detergents allows for visualization of the raft-like nature of the virus envelope.
Radyukhin V, Fedorova N, Ksenofontov A, Serebryakova M, Baratova L. - Department of Chromatography, A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Leninskie Gory, Moscow, 119992, Russia, varvic@belozersky.msu.ru.
Membrane solubilization with a mixture of cold non-ionic detergents has been applied to isolate detergent-resistant membranes from intact virus A lipid bilayer.
Association of the viral envelope glycoproteins and M1 into a raft lipid-protein complex was verified via detergent insolubility experiments, and the M1:HA stoichiometry of the proposed supramolecular complex was estimated via amino acid analysis.
Electron microscopy and dynamic light scattering data revealed that these lipid-protein rafts form unilamellar vesicles with HA spikes on their surfaces similar to influenza virus virions.
Together, our data suggest that the cold co-extraction technique visualizes the raft-like nature of the viral envelope and demonstrates the interaction of matrix M1 protein with the envelope.
PMID: 18825482 [PubMed - as supplied by publisher]
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