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Nat Biotechnol. 2014 Nov 17. doi: 10.1038/nbt.3079. [Epub ahead of print]
The draft genome sequence of the ferret (Mustela putorius furo) facilitates study of human respiratory disease.
Peng X1, Alf?ldi J2, Gori K3, Eisfeld AJ4, Tyler SR5, Tisoncik-Go J1, Brawand D6, Law GL1, Skunca N7, Hatta M4, Gasper DJ4, Kelly SM1, Chang J1, Thomas MJ1, Johnson J2, Berlin AM2, Lara M6, Russell P6, Swofford R2, Turner-Maier J2, Young S2, Hourlier T8, Aken B8, Searle S8, Sun X9, Yi Y9, Suresh M4, Tumpey TM10, Siepel A11, Wisely SM12, Dessimoz C13, Kawaoka Y14, Birren BW2, Lindblad-Toh K15, Di Palma F6, Engelhardt JF16, Palermo RE1, Katze MG17.
Author information
Abstract
The domestic ferret (Mustela putorius furo) is an important animal model for multiple human respiratory diseases. It is considered the 'gold standard' for modeling human influenza virus infection and transmission. Here we describe the 2.41 Gb draft genome assembly of the domestic ferret, constituting 2.28 Gb of sequence plus gaps. We annotated 19,910 protein-coding genes on this assembly using RNA-seq data from 21 ferret tissues. We characterized the ferret host response to two influenza virus infections by RNA-seq analysis of 42 ferret samples from influenza time-course data and showed distinct signatures in ferret trachea and lung tissues specific to 1918 or 2009 human pandemic influenza virus infections. Using microarray data from 16 ferret samples reflecting cystic fibrosis disease progression, we showed that transcriptional changes in the CFTR-knockout ferret lung reflect pathways of early disease that cannot be readily studied in human infants with cystic fibrosis disease.
PMID:
25402615
[PubMed - as supplied by publisher]
The draft genome sequence of the ferret (Mustela putorius furo) facilitates study of human respiratory disease.
Peng X1, Alf?ldi J2, Gori K3, Eisfeld AJ4, Tyler SR5, Tisoncik-Go J1, Brawand D6, Law GL1, Skunca N7, Hatta M4, Gasper DJ4, Kelly SM1, Chang J1, Thomas MJ1, Johnson J2, Berlin AM2, Lara M6, Russell P6, Swofford R2, Turner-Maier J2, Young S2, Hourlier T8, Aken B8, Searle S8, Sun X9, Yi Y9, Suresh M4, Tumpey TM10, Siepel A11, Wisely SM12, Dessimoz C13, Kawaoka Y14, Birren BW2, Lindblad-Toh K15, Di Palma F6, Engelhardt JF16, Palermo RE1, Katze MG17.
Author information
Abstract
The domestic ferret (Mustela putorius furo) is an important animal model for multiple human respiratory diseases. It is considered the 'gold standard' for modeling human influenza virus infection and transmission. Here we describe the 2.41 Gb draft genome assembly of the domestic ferret, constituting 2.28 Gb of sequence plus gaps. We annotated 19,910 protein-coding genes on this assembly using RNA-seq data from 21 ferret tissues. We characterized the ferret host response to two influenza virus infections by RNA-seq analysis of 42 ferret samples from influenza time-course data and showed distinct signatures in ferret trachea and lung tissues specific to 1918 or 2009 human pandemic influenza virus infections. Using microarray data from 16 ferret samples reflecting cystic fibrosis disease progression, we showed that transcriptional changes in the CFTR-knockout ferret lung reflect pathways of early disease that cannot be readily studied in human infants with cystic fibrosis disease.
PMID:
25402615
[PubMed - as supplied by publisher]
