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Sequences from Tamiflu Resistant Immunocompromised Patients Released
- Thread starter HenryN
- Start date
Re: Sequences from Tamiflu Resistant Immunocompromised Patients Released
EpiFlu Isolate ID
EPI_ISL_34538
Name and synonyms
A/Washington/29/2009
Type/subtype lineage
A/H1N1 swl
Passage history
X
Sample collection date
2009-07-28
Host
Human
Location
USA - Washington state
First submission
2009-08-21
Last update
EpiFlu Isolate ID
EPI_ISL_34538
Name and synonyms
A/Washington/29/2009
Type/subtype lineage
A/H1N1 swl
Passage history
X
Sample collection date
2009-07-28
Host
Human
Location
USA - Washington state
First submission
2009-08-21
Last update
Re: Sequences from Tamiflu Resistant Immunocompromised Patients Released
EpiFlu Isolate ID
EPI_ISL_34537
Name and synonyms
A/Washington/28/2009
Type/subtype lineage
A/H1N1 swl
Passage history
X
Sample collection date
2009-07-14
Host
Human
Location
USA - Washington state
First submission
2009-08-21
Last update
EpiFlu Isolate ID
EPI_ISL_34537
Name and synonyms
A/Washington/28/2009
Type/subtype lineage
A/H1N1 swl
Passage history
X
Sample collection date
2009-07-14
Host
Human
Location
USA - Washington state
First submission
2009-08-21
Last update
Re: Sequences from Tamiflu Resistant Immunocompromised Patients Released
Both viruses fall into the CDC variant i category (concensus strain), however, each has some unique polymorphisms:
Washington/28 HA matches Hong Kong swine (2009).
Washington/29 NA matches Florida human Swine Flu (04/2009 and 08/2009).
Both viruses fall into the CDC variant i category (concensus strain), however, each has some unique polymorphisms:
Washington/28 HA matches Hong Kong swine (2009).
Washington/29 NA matches Florida human Swine Flu (04/2009 and 08/2009).
Re: Sequences from Tamiflu Resistant Immunocompromised Patients Released
But both NA sequences are MIXTURES and SHARE the same polymorphism (in addition to H274Y)!
Re: Sequences from Tamiflu Resistant Immunocompromised Patients Released
Well, unless I'm going blind, Wash/29 has S82P, and Wash/28 does not.
The only other Swine Flu isolates I can find that have S82P are Florida/04 and 08.
Well, unless I'm going blind, Wash/29 has S82P, and Wash/28 does not.
The only other Swine Flu isolates I can find that have S82P are Florida/04 and 08.
gjs47
Well-known member
Re: Sequences from Tamiflu Resistant Immunocompromised Patients Released
This statement is confusing....
This statement is confusing....
Last edited by a moderator:
Re: Sequences from Tamiflu Resistant Immunocompromised Patients Released
I appoligize for being so brief.
Washington/29 has a unique change in the NA segment that no other Swine Flu isolate has except for two Florida isolates. That change is a Proline at position 82.
So, I was simply attempting to clarify that while the two Washington isolates may in fact be "mixtures", and perhaps "share the same polymorphism", they are clearly not the same. In other words do not share all the same polymorphisms.
I appoligize for being so brief.
Washington/29 has a unique change in the NA segment that no other Swine Flu isolate has except for two Florida isolates. That change is a Proline at position 82.
So, I was simply attempting to clarify that while the two Washington isolates may in fact be "mixtures", and perhaps "share the same polymorphism", they are clearly not the same. In other words do not share all the same polymorphisms.
Last edited:
Re: Sequences from Tamiflu Resistant Immunocompromised Patients Released
gb|CY044165.1| Influenza A virus (A/Mexico/48N/2009(H1N1)) se... 36.2 0.73
gb|GQ433898.1| Influenza A virus (A/Jiangsu/1/2009(H1N1)) seg... 36.2 0.73
gb|GQ377086.1| Influenza A virus (A/Oregon/07/2009(H1N1)) seg... 36.2 0.73
gb|GQ377074.1| Influenza A virus (A/Washington/18/2009(H1N1))... 36.2 0.73
gb|GQ377048.1| Influenza A virus (A/Washington/17/2009(H1N1))... 36.2 0.73
gb|GQ338393.1| Influenza A virus (A/Washington/08/2009(H1N1))... 36.2 0.73
gb|GQ338371.1| Influenza A virus (A/Washington/10/2009(H1N1))... 36.2 0.73
gb|GQ323562.1| Influenza A virus (A/Florida/10/2009(H1N1)) se... 36.2 0.73
gb|GQ323543.1| Influenza A virus (A/Washington/09/2009(H1N1))... 36.2 0.73
gb|GQ323507.1| Influenza A virus (A/Washington/14/2009(H1N1))... 36.2 0.73
gb|GQ223445.1| Influenza A virus (A/GuangzhouSB/01/2009(H1N1)... 36.2 0.73
gb|GQ221696.1| Influenza A virus (A/GuangzhouSB/01/2009(H1N1)... 36.2 0.73
gb|CY040890.1| Influenza A virus (A/Mexico/47N/2009(H1N1)) se... 36.2 0.73
gb|GQ150333.1| Influenza A virus (A/Christchurch/2/2009(H1N1)... 36.2 0.73
gb|GQ132158.1| Influenza A virus (A/Canada-NS/RV1536/2009(H1N... 36.2 0.73
gb|GQ132154.1| Influenza A virus (A/Canada-NS/RV1538/2009(H1N... 36.2 0.73
gb|GQ122098.1| Influenza A virus (A/Christchurch/2/2009(H1N1)... 36.2 0.73
gb|FJ998213.1| Influenza A virus (A/Canada-NS/RV1535/2009(H1N... 36.2 0.73
But no one said they were the same. Both NA sequences have mixed signals and both have this travel log (in addition to H274Y)
gb|CY044165.1| Influenza A virus (A/Mexico/48N/2009(H1N1)) se... 36.2 0.73
gb|GQ433898.1| Influenza A virus (A/Jiangsu/1/2009(H1N1)) seg... 36.2 0.73
gb|GQ377086.1| Influenza A virus (A/Oregon/07/2009(H1N1)) seg... 36.2 0.73
gb|GQ377074.1| Influenza A virus (A/Washington/18/2009(H1N1))... 36.2 0.73
gb|GQ377048.1| Influenza A virus (A/Washington/17/2009(H1N1))... 36.2 0.73
gb|GQ338393.1| Influenza A virus (A/Washington/08/2009(H1N1))... 36.2 0.73
gb|GQ338371.1| Influenza A virus (A/Washington/10/2009(H1N1))... 36.2 0.73
gb|GQ323562.1| Influenza A virus (A/Florida/10/2009(H1N1)) se... 36.2 0.73
gb|GQ323543.1| Influenza A virus (A/Washington/09/2009(H1N1))... 36.2 0.73
gb|GQ323507.1| Influenza A virus (A/Washington/14/2009(H1N1))... 36.2 0.73
gb|GQ223445.1| Influenza A virus (A/GuangzhouSB/01/2009(H1N1)... 36.2 0.73
gb|GQ221696.1| Influenza A virus (A/GuangzhouSB/01/2009(H1N1)... 36.2 0.73
gb|CY040890.1| Influenza A virus (A/Mexico/47N/2009(H1N1)) se... 36.2 0.73
gb|GQ150333.1| Influenza A virus (A/Christchurch/2/2009(H1N1)... 36.2 0.73
gb|GQ132158.1| Influenza A virus (A/Canada-NS/RV1536/2009(H1N... 36.2 0.73
gb|GQ132154.1| Influenza A virus (A/Canada-NS/RV1538/2009(H1N... 36.2 0.73
gb|GQ122098.1| Influenza A virus (A/Christchurch/2/2009(H1N1)... 36.2 0.73
gb|FJ998213.1| Influenza A virus (A/Canada-NS/RV1535/2009(H1N... 36.2 0.73
Re: Sequences from Tamiflu Resistant Immunocompromised Patients Released
Oh, I see the problem. Yes, there are two patients with Tamiflu resistant viruses. And I made a typo. Washington/28 does not have the S82P change in NA.
I did not wish to put words into anyone's mouth, only to clarify that while the two Washington isolates might share polymorphisms, they do not share all polymorphisms.
And I guess I screwed that up pretty well, didn't I?
Oh, I see the problem. Yes, there are two patients with Tamiflu resistant viruses. And I made a typo. Washington/28 does not have the S82P change in NA.
I did not wish to put words into anyone's mouth, only to clarify that while the two Washington isolates might share polymorphisms, they do not share all polymorphisms.
And I guess I screwed that up pretty well, didn't I?
Re: Sequences from Tamiflu Resistant Immunocompromised Patients Released
I believe that was the choir that spent three weeks in Mexico.
I believe that was the choir that spent three weeks in Mexico.
wotan
Well-known member
Re: Sequences from Tamiflu Resistant Immunocompromised Patients Released
But who brought it back to us?
But who brought it back to us?
Re: Sequences from Tamiflu Resistant Immunocompromised Patients Released
Don't look at me.
I've been trying to get the Swine Flu for three months while it is still relatively mild, but to no avail. My daughter had it, but refused to share.
But who brought it back to us?
Don't look at me.
I've been trying to get the Swine Flu for three months while it is still relatively mild, but to no avail. My daughter had it, but refused to share.
Rwilmer
Well-known member
Re: Sequences from Tamiflu Resistant Immunocompromised Patients Released
Might not do you much good. As we are seeing indications of reinfection in mexico.
Don't look at me.
I've been trying to get the Swine Flu for three months while it is still relatively mild, but to no avail. My daughter had it, but refused to share.
Might not do you much good. As we are seeing indications of reinfection in mexico.
Re: Sequences from Tamiflu Resistant Immunocompromised Patients Released
LOCUS GQ499337 1410 bp cRNA linear VRL 21-AUG-2009
DEFINITION Influenza A virus (A/Washington/28/2009(H1N1)) segment 6
neuraminidase (NA) gene, complete cds.
ACCESSION GQ499337
VERSION GQ499337.1 GI:256383636
DBLINK Project:37813
KEYWORDS .
SOURCE Influenza A virus (A/Washington/28/2009(H1N1))
ORGANISM Influenza A virus (A/Washington/28/2009(H1N1))
Viruses; ssRNA negative-strand viruses; Orthomyxoviridae;
Influenzavirus A.
REFERENCE 1 (bases 1 to 1410)
AUTHORS Deyde,V., Sheu,T., Okomo-Adhiambo,M., Hillman,M., Barnes,J.,
Garten,R., Klimov,A., Gubareva,L. and Cox,N.
TITLE Oseltamivir-Resistant Novel Influenza A (H1N1) Virus Infection in
Immunosuppressed Patients Receiving Oseltamivir Therapy
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1410)
AUTHORS Deyde,V., Sheu,T., Okomo-Adhiambo,M., Hillman,M., Barnes,J.,
Garten,R., Klimov,A., Gubareva,L. and Cox,N.
TITLE Direct Submission
JOURNAL Submitted (21-AUG-2009) WHO Collaborating Center for Surveillance,
Epidemiology and Control of Influenza, Influenza Division, Centers
for Disease Control and Prevention, 1600 Clifton Road, N.E.,
Atlanta, GA 30333, USA
COMMENT Swine influenza A (H1N1) virus isolated during human swine flu
outbreak of 2009. For more information, see http://www.cdc.gov/.
Some of the information does not have GenBank feature identifiers
and is being provided in the comment section.
##EpifluData-START##
Isolate A/Washington/28/2009
Subtype H1N1
Segment_name NA
Host_gender M
Host_age 18
Passage_history X
Adamantane_resistance resistant
Zanamivir_resistance sensitive
Oseltamivir_resistance resistant
Country USA
State/Province Washington state
Collection_day 14
Collection_month 7
Collection_year 2009
Isolate_note Human case of H1N1 pandemic influenza with
Oseltamivir Resistance.
EPI_accession EPI191936
Lineage swl
##EpifluData-END##
FEATURES Location/Qualifiers
source 1..1410
/organism="Influenza A virus (A/Washington/28/2009(H1N1))"
/mol_type="viral cRNA"
/strain="A/Washington/28/2009"
/serotype="H1N1"
/host="Homo sapiens; gender: M; age: 18"
/db_xref="taxon:667724"
/segment="6"
/country="USA: Washington state"
/collection_date="14-Jul-2009"
gene 1..1410
/gene="NA"
CDS 1..1410
/gene="NA"
/codon_start=1
/product="neuraminidase"
/protein_id="ACU78208.1"
/db_xref="GI:256383637"
/translation="MNPNQKIITIGSVCMTIGMANLILQIGNIISIWISHSIQLGNQN
QIETCNQSVITYENNTWVNQTYVNISNTNFAAGQSVVSVKLAGNSSLCPVSGWAIYSK
DNSVRIGSKGDVFVIREPFISCSPXECRTFFLTQGALLNDKHSNGTIKDRSPYRTLMS
CPIGEVPSPYNSRFESVAWSASACHDGINWLTIGISGPDNGAVAVLKYNGIITDTIKS
WRNNILRTQESECACVNGSCFTVMTDGPSNGQASYKIFRIEKGKIVKSVEMNAPNYYY
EECSCYPDSSEITCVCRDNWHGSNRPWVSFNQNLEYQIGYICSGIFGDNPRPNDKTGS
CGPVSSNGANGVKGFSFKYGNGVWIGRTKSISSRNGFEMIWDPNGWTGTDNNFSIKQD
IVGINEWSGYSGSFVQHPELTGLDCIRPCFWVELIRGRPKENTIWTSGSSISFCGVNS
DTVGWSWPDGAELPFTIDK"
ORIGIN
1 atgaatccaa accaaaagat aataaccatt ggttcggtct gtatgacaat tggaatggct
61 aacttaatat tacaaattgg aaacataatc tcaatatgga ttagccactc aattcaactt
121 gggaatcaaa atcagattga aacatgcaat caaagcgtca ttacttatga aaacaacact
181 tgggtaaatc agacatatgt taacatcagc aacaccaact ttgctgctgg acagtcagtg
241 gtttccgtga aattagcggg caattcctct ctctgccctg ttagtggatg ggctatatac
301 agtaaagaca acagtgtaag aatcggttcc aagggggatg tgtttgtcat aagggaacca
361 ttcatatcat gctcccccty ggaatgcaga accttcttct tgactcaagg ggccttgcta
421 aatgacaaac attccaatgg aaccattaaa gacaggagcc catatcgaac cctaatgagc
481 tgtcctattg gtgaagttcc ctctccatac aactcaagat ttgagtcagt cgcttggtca
541 gcaagtgctt gtcatgatgg catcaattgg ctaacaattg gaatttctgg cccagacaat
601 ggggcagtgg ctgtgttaaa gtacaacggc ataataacag acactatcaa gagttggaga
661 aacaatatat tgagaacaca agagtctgaa tgtgcatgtg taaatggttc ttgctttact
721 gtaatgaccg atggaccaag taatggacag gcctcataca agatcttcag aatagaaaag
781 ggaaagatag tcaaatcagt cgaaatgaat gcccctaatt attactatga ggaatgctcc
841 tgttatcctg attctagtga aatcacatgt gtgtgcaggg ataactggca tggctcgaat
901 cgaccgtggg tgtctttcaa ccagaatctg gaatatcaga taggatacat atgcagtggg
961 attttcggag acaatccacg ccctaatgat aagacaggca gttgtggtcc agtatcgtct
1021 aatggagcaa atggagtaaa aggattttca ttcaaatacg gcaatggtgt ttggataggg
1081 agaactaaaa gcattagttc aagaaacggt tttgagatga tttgggatcc gaacggatgg
1141 actgggacag acaataactt ctcaataaag caagatatcg taggaataaa tgagtggtca
1201 ggatatagcg ggagttttgt tcagcatccg gaactaacag ggctggattg tataagacct
1261 tgcttctggg ttgaactaat cagagggcga cccaaagaga acacaatctg gactagcggg
1321 agcagcatat ccttttgtgg tgtaaacagt gacactgtgg gttggtcttg gccagacggt
1381 gctgagttgc catttaccat tgacaagtaa
LOCUS GQ499337 1410 bp cRNA linear VRL 21-AUG-2009
DEFINITION Influenza A virus (A/Washington/28/2009(H1N1)) segment 6
neuraminidase (NA) gene, complete cds.
ACCESSION GQ499337
VERSION GQ499337.1 GI:256383636
DBLINK Project:37813
KEYWORDS .
SOURCE Influenza A virus (A/Washington/28/2009(H1N1))
ORGANISM Influenza A virus (A/Washington/28/2009(H1N1))
Viruses; ssRNA negative-strand viruses; Orthomyxoviridae;
Influenzavirus A.
REFERENCE 1 (bases 1 to 1410)
AUTHORS Deyde,V., Sheu,T., Okomo-Adhiambo,M., Hillman,M., Barnes,J.,
Garten,R., Klimov,A., Gubareva,L. and Cox,N.
TITLE Oseltamivir-Resistant Novel Influenza A (H1N1) Virus Infection in
Immunosuppressed Patients Receiving Oseltamivir Therapy
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1410)
AUTHORS Deyde,V., Sheu,T., Okomo-Adhiambo,M., Hillman,M., Barnes,J.,
Garten,R., Klimov,A., Gubareva,L. and Cox,N.
TITLE Direct Submission
JOURNAL Submitted (21-AUG-2009) WHO Collaborating Center for Surveillance,
Epidemiology and Control of Influenza, Influenza Division, Centers
for Disease Control and Prevention, 1600 Clifton Road, N.E.,
Atlanta, GA 30333, USA
COMMENT Swine influenza A (H1N1) virus isolated during human swine flu
outbreak of 2009. For more information, see http://www.cdc.gov/.
Some of the information does not have GenBank feature identifiers
and is being provided in the comment section.
##EpifluData-START##
Isolate A/Washington/28/2009
Subtype H1N1
Segment_name NA
Host_gender M
Host_age 18
Passage_history X
Adamantane_resistance resistant
Zanamivir_resistance sensitive
Oseltamivir_resistance resistant
Country USA
State/Province Washington state
Collection_day 14
Collection_month 7
Collection_year 2009
Isolate_note Human case of H1N1 pandemic influenza with
Oseltamivir Resistance.
EPI_accession EPI191936
Lineage swl
##EpifluData-END##
FEATURES Location/Qualifiers
source 1..1410
/organism="Influenza A virus (A/Washington/28/2009(H1N1))"
/mol_type="viral cRNA"
/strain="A/Washington/28/2009"
/serotype="H1N1"
/host="Homo sapiens; gender: M; age: 18"
/db_xref="taxon:667724"
/segment="6"
/country="USA: Washington state"
/collection_date="14-Jul-2009"
gene 1..1410
/gene="NA"
CDS 1..1410
/gene="NA"
/codon_start=1
/product="neuraminidase"
/protein_id="ACU78208.1"
/db_xref="GI:256383637"
/translation="MNPNQKIITIGSVCMTIGMANLILQIGNIISIWISHSIQLGNQN
QIETCNQSVITYENNTWVNQTYVNISNTNFAAGQSVVSVKLAGNSSLCPVSGWAIYSK
DNSVRIGSKGDVFVIREPFISCSPXECRTFFLTQGALLNDKHSNGTIKDRSPYRTLMS
CPIGEVPSPYNSRFESVAWSASACHDGINWLTIGISGPDNGAVAVLKYNGIITDTIKS
WRNNILRTQESECACVNGSCFTVMTDGPSNGQASYKIFRIEKGKIVKSVEMNAPNYYY
EECSCYPDSSEITCVCRDNWHGSNRPWVSFNQNLEYQIGYICSGIFGDNPRPNDKTGS
CGPVSSNGANGVKGFSFKYGNGVWIGRTKSISSRNGFEMIWDPNGWTGTDNNFSIKQD
IVGINEWSGYSGSFVQHPELTGLDCIRPCFWVELIRGRPKENTIWTSGSSISFCGVNS
DTVGWSWPDGAELPFTIDK"
ORIGIN
1 atgaatccaa accaaaagat aataaccatt ggttcggtct gtatgacaat tggaatggct
61 aacttaatat tacaaattgg aaacataatc tcaatatgga ttagccactc aattcaactt
121 gggaatcaaa atcagattga aacatgcaat caaagcgtca ttacttatga aaacaacact
181 tgggtaaatc agacatatgt taacatcagc aacaccaact ttgctgctgg acagtcagtg
241 gtttccgtga aattagcggg caattcctct ctctgccctg ttagtggatg ggctatatac
301 agtaaagaca acagtgtaag aatcggttcc aagggggatg tgtttgtcat aagggaacca
361 ttcatatcat gctcccccty ggaatgcaga accttcttct tgactcaagg ggccttgcta
421 aatgacaaac attccaatgg aaccattaaa gacaggagcc catatcgaac cctaatgagc
481 tgtcctattg gtgaagttcc ctctccatac aactcaagat ttgagtcagt cgcttggtca
541 gcaagtgctt gtcatgatgg catcaattgg ctaacaattg gaatttctgg cccagacaat
601 ggggcagtgg ctgtgttaaa gtacaacggc ataataacag acactatcaa gagttggaga
661 aacaatatat tgagaacaca agagtctgaa tgtgcatgtg taaatggttc ttgctttact
721 gtaatgaccg atggaccaag taatggacag gcctcataca agatcttcag aatagaaaag
781 ggaaagatag tcaaatcagt cgaaatgaat gcccctaatt attactatga ggaatgctcc
841 tgttatcctg attctagtga aatcacatgt gtgtgcaggg ataactggca tggctcgaat
901 cgaccgtggg tgtctttcaa ccagaatctg gaatatcaga taggatacat atgcagtggg
961 attttcggag acaatccacg ccctaatgat aagacaggca gttgtggtcc agtatcgtct
1021 aatggagcaa atggagtaaa aggattttca ttcaaatacg gcaatggtgt ttggataggg
1081 agaactaaaa gcattagttc aagaaacggt tttgagatga tttgggatcc gaacggatgg
1141 actgggacag acaataactt ctcaataaag caagatatcg taggaataaa tgagtggtca
1201 ggatatagcg ggagttttgt tcagcatccg gaactaacag ggctggattg tataagacct
1261 tgcttctggg ttgaactaat cagagggcga cccaaagaga acacaatctg gactagcggg
1321 agcagcatat ccttttgtgg tgtaaacagt gacactgtgg gttggtcttg gccagacggt
1381 gctgagttgc catttaccat tgacaagtaa
Re: Sequences from Tamiflu Resistant Immunocompromised Patients Released
LOCUS GQ499335 1410 bp cRNA linear VRL 21-AUG-2009
DEFINITION Influenza A virus (A/Washington/29/2009(H1N1)) segment 6
neuraminidase (NA) gene, complete cds.
ACCESSION GQ499335
VERSION GQ499335.1 GI:256383632
DBLINK Project:37813
KEYWORDS .
SOURCE Influenza A virus (A/Washington/29/2009(H1N1))
ORGANISM Influenza A virus (A/Washington/29/2009(H1N1))
Viruses; ssRNA negative-strand viruses; Orthomyxoviridae;
Influenzavirus A.
REFERENCE 1 (bases 1 to 1410)
AUTHORS Deyde,V., Sheu,T., Okomo-Adhiambo,M., Hillman,M., Barnes,J.,
Garten,R., Klimov,A., Gubareva,L. and Cox,N.
TITLE Oseltamivir-Resistant Novel Influenza A (H1N1) Virus Infection in
Immunosuppressed Patients Receiving Oseltamivir Therapy
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1410)
AUTHORS Deyde,V., Sheu,T., Okomo-Adhiambo,M., Hillman,M., Barnes,J.,
Garten,R., Klimov,A., Gubareva,L. and Cox,N.
TITLE Direct Submission
JOURNAL Submitted (21-AUG-2009) WHO Collaborating Center for Surveillance,
Epidemiology and Control of Influenza, Influenza Division, Centers
for Disease Control and Prevention, 1600 Clifton Road, N.E.,
Atlanta, GA 30333, USA
COMMENT Swine influenza A (H1N1) virus isolated during human swine flu
outbreak of 2009. For more information, see http://www.cdc.gov/.
Some of the information does not have GenBank feature identifiers
and is being provided in the comment section.
##EpifluData-START##
Isolate A/Washington/29/2009
Subtype H1N1
Segment_name NA
Host_gender F
Host_age 47
Passage_history X
Adamantane_resistance resistant
Zanamivir_resistance sensitive
Oseltamivir_resistance resistant
Country USA
State/Province Washington state
Collection_day 28
Collection_month 7
Collection_year 2009
Isolate_note Human case of H1N1 pandemic influenza with
Oseltamivir resistance.
EPI_accession EPI191938
Lineage swl
##EpifluData-END##
FEATURES Location/Qualifiers
source 1..1410
/organism="Influenza A virus (A/Washington/29/2009(H1N1))"
/mol_type="viral cRNA"
/strain="A/Washington/29/2009"
/serotype="H1N1"
/host="Homo sapiens; gender: F; age: 47"
/db_xref="taxon:667723"
/segment="6"
/country="USA: Washington state"
/collection_date="28-Jul-2009"
gene 1..1410
/gene="NA"
CDS 1..1410
/gene="NA"
/codon_start=1
/product="neuraminidase"
/protein_id="ACU78206.1"
/db_xref="GI:256383633"
/translation="MNPNQKIITIGSVCMTIGMANLILQIGNIISIWISHSIQLGNQN
QIETCNQSVITYENNTWVNQTYVNISNTNFAAGQSVVPVKLAGNSSLCPVSGWAIYSK
DNSVRIGSKGDVFVIREPFISCSPLECRTFFLTQGALLNDKHSNGTIKDRSPYRTLMS
CPIGEVPSPYNSRFESVAWSASACHDGINWLTIGISGPDNGAVAVLKYNGIITDTIKS
WRNNILRTQESECACVNGSCFTVMTDGPSNGQASYKIFXIEKGKIVKSVEMNAPNYYY
EECSCYPDSSEITCVCRDNWHGSNRPWVSFNQNLEYQIGYICSGIFGDNPRPNDKTGS
CDPVSSNGANGVKGFSFKYGNGVWIGRTKSISSRNGFEMIWDPNGWTGTDNNFSIKQD
IVGINEWSGYSGSFVQHPELTGLDCIRPCFWVELIRGRPKENTIWTSGSSISFCGVNS
DTVGWSWPDGAELPFTIDK"
ORIGIN
1 atgaatccaa accaaaagat aataaccatt ggttcggtct gtatgacaat tggaatggct
61 aacttaatat tacaaattgg aaacataatc tcaatatgga ttagccactc aattcaactt
121 gggaatcaaa atcagattga aacatgcaat caaagcgtca ttacttatga aaacaacact
181 tgggtaaatc agacatatgt taacatcagc aacaccaact ttgctgctgg acagtcagtg
241 gttcccgtga aattagcggg caattcctct ctctgccctg ttagtggatg ggctatatac
301 agtaaagaca acagtgtaag aatcggttcc aagggggatg tgtttgtcat aagggaacca
361 ttcatatcat gctccccctt ggaatgcaga accttcttct tgactcaagg ggccttgcta
421 aatgacaaac attccaatgg aaccattaaa gacaggagcc catatcgaac cctaatgagc
481 tgtcctattg gtgaagttcc ctctccatac aactcaagat ttgagtcagt cgcttggtca
541 gcaagtgctt gtcatgatgg catcaattgg ctaacaattg gaatttctgg cccagacaat
601 ggggcagtgg ctgtgttaaa gtacaacggc ataataacag acactatcaa gagttggaga
661 aacaatatat tgagaacaca agagtctgaa tgtgcatgtg taaatggttc ttgctttact
721 gtaatgaccg atggaccaag taatggacag gcctcataca agatcttcar aatagaaaag
781 ggaaagatag tcaaatcagt cgaaatgaat gcccctaatt attactatga ggaatgctcc
841 tgttatcctg attctagtga aatcacatgt gtgtgcaggg ataactggca tggctcgaat
901 cgaccgtggg tatctttcaa ccagaatctg gaatatcaga taggatacat atgcagtggg
961 attttcggag acaatccacg ccctaatgat aagacaggca gttgtgatcc agtatcgtct
1021 aatggagcaa atggagtaaa aggattttca ttcaaatacg gcaatggtgt ttggataggg
1081 agaactaaaa gcattagttc aagaaacggt tttgagatga tttgggatcc gaacggatgg
1141 actgggacag acaataactt ctcaataaag caagatatcg taggaataaa tgagtggtca
1201 ggatatagcg ggagttttgt tcagcatccg gaactaacag ggctggattg tataagacct
1261 tgcttctggg ttgaactaat cagagggcga cccaaagaga acacaatctg gactagcggg
1321 agcagcatat ccttttgtgg tgtaaacagt gacactgtgg gttggtcttg gccagacggt
1381 gctgagttgc catttaccat tgacaagtaa
LOCUS GQ499335 1410 bp cRNA linear VRL 21-AUG-2009
DEFINITION Influenza A virus (A/Washington/29/2009(H1N1)) segment 6
neuraminidase (NA) gene, complete cds.
ACCESSION GQ499335
VERSION GQ499335.1 GI:256383632
DBLINK Project:37813
KEYWORDS .
SOURCE Influenza A virus (A/Washington/29/2009(H1N1))
ORGANISM Influenza A virus (A/Washington/29/2009(H1N1))
Viruses; ssRNA negative-strand viruses; Orthomyxoviridae;
Influenzavirus A.
REFERENCE 1 (bases 1 to 1410)
AUTHORS Deyde,V., Sheu,T., Okomo-Adhiambo,M., Hillman,M., Barnes,J.,
Garten,R., Klimov,A., Gubareva,L. and Cox,N.
TITLE Oseltamivir-Resistant Novel Influenza A (H1N1) Virus Infection in
Immunosuppressed Patients Receiving Oseltamivir Therapy
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1410)
AUTHORS Deyde,V., Sheu,T., Okomo-Adhiambo,M., Hillman,M., Barnes,J.,
Garten,R., Klimov,A., Gubareva,L. and Cox,N.
TITLE Direct Submission
JOURNAL Submitted (21-AUG-2009) WHO Collaborating Center for Surveillance,
Epidemiology and Control of Influenza, Influenza Division, Centers
for Disease Control and Prevention, 1600 Clifton Road, N.E.,
Atlanta, GA 30333, USA
COMMENT Swine influenza A (H1N1) virus isolated during human swine flu
outbreak of 2009. For more information, see http://www.cdc.gov/.
Some of the information does not have GenBank feature identifiers
and is being provided in the comment section.
##EpifluData-START##
Isolate A/Washington/29/2009
Subtype H1N1
Segment_name NA
Host_gender F
Host_age 47
Passage_history X
Adamantane_resistance resistant
Zanamivir_resistance sensitive
Oseltamivir_resistance resistant
Country USA
State/Province Washington state
Collection_day 28
Collection_month 7
Collection_year 2009
Isolate_note Human case of H1N1 pandemic influenza with
Oseltamivir resistance.
EPI_accession EPI191938
Lineage swl
##EpifluData-END##
FEATURES Location/Qualifiers
source 1..1410
/organism="Influenza A virus (A/Washington/29/2009(H1N1))"
/mol_type="viral cRNA"
/strain="A/Washington/29/2009"
/serotype="H1N1"
/host="Homo sapiens; gender: F; age: 47"
/db_xref="taxon:667723"
/segment="6"
/country="USA: Washington state"
/collection_date="28-Jul-2009"
gene 1..1410
/gene="NA"
CDS 1..1410
/gene="NA"
/codon_start=1
/product="neuraminidase"
/protein_id="ACU78206.1"
/db_xref="GI:256383633"
/translation="MNPNQKIITIGSVCMTIGMANLILQIGNIISIWISHSIQLGNQN
QIETCNQSVITYENNTWVNQTYVNISNTNFAAGQSVVPVKLAGNSSLCPVSGWAIYSK
DNSVRIGSKGDVFVIREPFISCSPLECRTFFLTQGALLNDKHSNGTIKDRSPYRTLMS
CPIGEVPSPYNSRFESVAWSASACHDGINWLTIGISGPDNGAVAVLKYNGIITDTIKS
WRNNILRTQESECACVNGSCFTVMTDGPSNGQASYKIFXIEKGKIVKSVEMNAPNYYY
EECSCYPDSSEITCVCRDNWHGSNRPWVSFNQNLEYQIGYICSGIFGDNPRPNDKTGS
CDPVSSNGANGVKGFSFKYGNGVWIGRTKSISSRNGFEMIWDPNGWTGTDNNFSIKQD
IVGINEWSGYSGSFVQHPELTGLDCIRPCFWVELIRGRPKENTIWTSGSSISFCGVNS
DTVGWSWPDGAELPFTIDK"
ORIGIN
1 atgaatccaa accaaaagat aataaccatt ggttcggtct gtatgacaat tggaatggct
61 aacttaatat tacaaattgg aaacataatc tcaatatgga ttagccactc aattcaactt
121 gggaatcaaa atcagattga aacatgcaat caaagcgtca ttacttatga aaacaacact
181 tgggtaaatc agacatatgt taacatcagc aacaccaact ttgctgctgg acagtcagtg
241 gttcccgtga aattagcggg caattcctct ctctgccctg ttagtggatg ggctatatac
301 agtaaagaca acagtgtaag aatcggttcc aagggggatg tgtttgtcat aagggaacca
361 ttcatatcat gctccccctt ggaatgcaga accttcttct tgactcaagg ggccttgcta
421 aatgacaaac attccaatgg aaccattaaa gacaggagcc catatcgaac cctaatgagc
481 tgtcctattg gtgaagttcc ctctccatac aactcaagat ttgagtcagt cgcttggtca
541 gcaagtgctt gtcatgatgg catcaattgg ctaacaattg gaatttctgg cccagacaat
601 ggggcagtgg ctgtgttaaa gtacaacggc ataataacag acactatcaa gagttggaga
661 aacaatatat tgagaacaca agagtctgaa tgtgcatgtg taaatggttc ttgctttact
721 gtaatgaccg atggaccaag taatggacag gcctcataca agatcttcar aatagaaaag
781 ggaaagatag tcaaatcagt cgaaatgaat gcccctaatt attactatga ggaatgctcc
841 tgttatcctg attctagtga aatcacatgt gtgtgcaggg ataactggca tggctcgaat
901 cgaccgtggg tatctttcaa ccagaatctg gaatatcaga taggatacat atgcagtggg
961 attttcggag acaatccacg ccctaatgat aagacaggca gttgtgatcc agtatcgtct
1021 aatggagcaa atggagtaaa aggattttca ttcaaatacg gcaatggtgt ttggataggg
1081 agaactaaaa gcattagttc aagaaacggt tttgagatga tttgggatcc gaacggatgg
1141 actgggacag acaataactt ctcaataaag caagatatcg taggaataaa tgagtggtca
1201 ggatatagcg ggagttttgt tcagcatccg gaactaacag ggctggattg tataagacct
1261 tgcttctggg ttgaactaat cagagggcga cccaaagaga acacaatctg gactagcggg
1321 agcagcatat ccttttgtgg tgtaaacagt gacactgtgg gttggtcttg gccagacggt
1381 gctgagttgc catttaccat tgacaagtaa
Re: Sequences from Tamiflu Resistant Immunocompromised Patients Released
Shared polymorphism signals silent transmission by minor population with H274Y.
Shared polymorphism signals silent transmission by minor population with H274Y.