Denmark Tamiflu Resistant Sequence Released

[gsgs]

Re: Denmark Tamiflu Resistant Sequence Released

I think it has 3 stop codons.
And even if they were replaced, it seems unlikely that the
rest could encode a useful protein.
And it would disturb the encoding of PB1


So it looks easier to me to get an entirely new segment 2
from some other virus
(classical swine)
or has human H1N1 PB1-F2, I forgot, but I assume not



BTW. what about N40

 

[Mamabird]

Re: Denmark Tamiflu Resistant Sequence Released

I think it has 3 stop codons.
And even if they were replaced, it seems unlikely that the
rest could encode a useful protein.
And it would disturb the encoding of PB1


So it looks easier to me to get an entirely new segment 2
from some other virus
(classical swine)
or has human H1N1 PB1-F2, I forgot, but I assume not



BTW. what about N40

GS: the stop condon is "taa" at position 128-130 of PB1 (11 amino acids into the F2 protein). It is the only one of which I am aware, and should you have one nucleiod change to drop the stop condon, then F2 should be produced with some nasty looking markers that closely track other problem viruses.

And yes, both H1N1 and H3N2 seasonal flu viruses code for the F2 protein.

As to "N40", you will have to enlighten me as to what that is or means. I'm not sure I understand that last question.

 

[HenryN]

Re: Denmark Tamiflu Resistant Sequence Released

I think it has 3 stop codons.
And even if they were replaced, it seems unlikely that the
rest could encode a useful protein.
And it would disturb the encoding of PB1


So it looks easier to me to get an entirely new segment 2
from some other virus
(classical swine)
or has human H1N1 PB1-F2, I forgot, but I assume not



BTW. what about N40

Please. Many flu genomes have no stops and have fully functional PB1 (which is the point).

 

[gsgs]

Re: Denmark Tamiflu Resistant Sequence Released

ahh, yes. Human flu has PB1-F2

classical swine H1N1 has not, but "triple reassortant, 1998" has, since PB1 is from human H3N2.
However it was lost and ** has none.

Presumably its useful for humans but not swine

So this possible reassortment is problematic

 

[HenryN]

Re: Denmark Tamiflu Resistant Sequence Released

ahh, yes. Human flu has PB1-F2

classical swine H1N1 has not, but "triple reassortant, 1998" has, since PB1 is from human H3N2.
However it was lost and ** has none.

Presumably its useful for humans but not swine

So this possible reassortment is problematic

Single nucleotide changes are FAR more common than reassortment.