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Denmark Tamiflu Resistant Sequence Released

Re: Denmark Tamiflu Resistant Sequence Released

LOCUS CY043352 1424 bp cRNA linear VRL 04-AUG-2009
DEFINITION Influenza A virus (A/Denmark/528/2009(H1N1)) segment 6 sequence.
ACCESSION CY043352
VERSION CY043352.1 GI:255103492
DBLINK Project:37813
KEYWORDS .
SOURCE Influenza A virus (A/Denmark/528/2009(H1N1))
ORGANISM Influenza A virus (A/Denmark/528/2009(H1N1))
Viruses; ssRNA negative-strand viruses; Orthomyxoviridae;
Influenzavirus A.
REFERENCE 1 (bases 1 to 1424)
AUTHORS Gregory,V., Nielson,L., Zheng,X., Whittaker,L., Daniels,R. and
Hay,A.
TITLE Pandemic influenza A(H1N1) virus sequences
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1424)
AUTHORS Gregory,V., Nielson,L., Zheng,X., Whittaker,L., Daniels,R. and
Hay,A.
TITLE Direct Submission
JOURNAL Submitted (04-AUG-2009) WHO Collaborating Centre for Reference and
Research on Influenza, National Institute for Medical Research, The
Ridgeway, Mill Hill, London NW7 1AA, UK
FEATURES Location/Qualifiers
source 1..1424
/organism="Influenza A virus (A/Denmark/528/2009(H1N1))"
/mol_type="viral cRNA"
/strain="A/Denmark/528/2009(H1N1)"
/serotype="H1N1"
/isolation_source="gender:Female; age:21y"
/host="human"
/db_xref="taxon:664181"
/segment="6"
/lab_host="swine kidney 2, MDCK 3 passage(s)"
/country="Denmark"
/collection_date="09-Jun-2009"
/note="Oseltamivir resistant, Zanamivir sensitive,
Amantidine resistant (31N in M2), lineage: swl"
gene 1..1410
/gene="NA"
CDS 1..1410
/gene="NA"
/codon_start=1
/product="neuraminidase"
/protein_id="ACU00954.1"
/db_xref="GI:255103493"
/translation="MNPNQKIITIGSVCMTIGMANLILQIGNIISIWISHSIQLGNQN
QIETCNQSVITYENNTWVNQTYVNISNTNFAAGQSVVSVKLAGNSSLCPVSGWAIYSK
DNSIRIGSKGDVFVIREPFISCSPLECRTFFLTQGALLNDKHSNGTIKDRSPYRTLMS
CPIGEVPSPYNSRFESVAWSASACHDGINWLTIGISGPDNGAVAVLKYNGIITDTIKS
WRNNILRTQESECACVNGSCFTVMTDGPSDGQASYKIFRIEKGKIVKSVEMNAPNYYY
EECSCYPDSSEITCVCRDNWHGSNRPWVSFNQNLEYQIGYICSGIFGDNPRPNDKTGS
CGPVSSNGANGVKGFSFKYGNGVWIGRTKSISSRNGFEMIWDPNGWTGTDNNFSIKQD
IVGINEWSGYSGSFVQHPELTGLDCIRPCFWVELIRGRPKENTIWTSGSSISFCGVNS
DTVGWSWPDGAELPFTIDK"
ORIGIN
1 atgaatccaa accaaaagat aataaccatt ggttcggtct gtatgacaat tggaatggct
61 aacttaatat tacaaattgg aaacataatc tcaatatgga ttagccactc aattcaactt
121 gggaatcaaa atcagattga aacatgcaat caaagcgtca ttacttatga aaacaacact
181 tgggtaaatc agacatatgt taacatcagc aacaccaact ttgctgctgg acagtcagtg
241 gtttccgtga aattagcggg caattcctct ctctgccctg ttagtggatg ggctatatac
301 agtaaagaca acagtataag aatcggttcc aagggggatg tgtttgtcat aagggaacca
361 ttcatatcat gctccccctt ggaatgcaga accttcttct tgactcaagg ggccttgcta
421 aatgacaaac attccaatgg aaccattaaa gacaggagcc catatcgaac cctaatgagc
481 tgtcctattg gtgaagttcc ctctccatac aactcaagat ttgagtcagt cgcttggtca
541 gcaagtgctt gtcatgatgg catcaattgg ctaacaattg gaatttctgg cccagacaat
601 ggggcagtgg ctgtgttaaa gtacaacggc ataataacag acactatcaa gagttggaga
661 aacaatatat tgagaacaca agagtctgaa tgtgcatgtg taaatggttc ttgctttact
721 gtaatgaccg atggaccaag tgatggacag gcctcataca agatcttcag aatagaaaag
781 ggaaagatag tcaaatcagt cgaaatgaat gcccctaatt attactatga ggaatgctcc
841 tgttatcctg attctagtga aatcacatgt gtgtgcaggg ataactggca tggctcgaat
901 cgaccgtggg tgtctttcaa ccagaatctg gaatatcaga taggatacat atgcagtggg
961 attttcggag acaatccacg ccctaatgat aagacaggca gttgtggtcc agtatcgtct
1021 aatggagcaa atggagtaaa aggattttca ttcaaatacg gcaatggtgt ttggataggg
1081 agaactaaaa gcattagttc aagaaacggt tttgagatga tttgggatcc gaacggatgg
1141 actgggacag acaataactt ctcaataaag caagatatcg taggaataaa tgagtggtca
1201 ggatatagcg ggagttttgt tcagcatcca gaactaacag ggctggattg tataagacct
1261 tgcttctggg ttgaactaat cagagggcga cccaaagaga acacaatctg gactagcggg
1321 agcagcatat ccttttgtgg tgtaaacagt gacactgtgg gttggtcttg gccagacggt
1381 gctgagttgc catttaccat tgacaagtaa tttgttcaaa aaac
 
Re: Denmark Tamiflu Resistant Sequence Released

H274Y travel log

gb|GQ397279.1| Influenza A virus (A/Yamaguchi/22/2009(H1N1)) ... 36.2 0.73
gb|GQ365445.1| Influenza A virus (A/Osaka/180/2009(H1N1)) seg... 36.2 0.73
gb|GQ351316.1| Influenza A virus (A/Hong Kong/2369/2009(H1N1)... 36.2 0.73
gb|EU567011.1| Influenza A virus (A/Indiana/01/2008(H1N1)) se... 36.2 0.73
gb|DQ493078.1| Influenza A virus (A/Vietnam/CL2009/2005(H5N1)... 36.2 0.73
gb|DQ250165.1| Influenza A virus (A/Vietnam/CL2009/2005(H5N1)... 36.2 0.73

Denmark is not listed because sequence is not yet in searchable database.
 
Re: Denmark Tamiflu Resistant Sequence Released

HA travel log

gb|CY040549.1| Influenza A virus (A/swine/North Carolina/4395... 32.2 11
gb|CY039991.1| Influenza A Virus (A/New Jersey/8/1976(H1N1)) ... 32.2 11
gb|FJ688266.1| Influenza A virus (A/swine/Thailand/HF6/2005(H... 32.2 11
dbj|AB434328.1| Influenza A virus (A/swine/Chachoengsao/NIAH5... 32.2 11
dbj|AB434320.1| Influenza A virus (A/swine/Chonburi/NIAH589/2... 32.2 11
dbj|AB434312.1| Influenza A virus (A/swine/Chonburi/NIAH977/2... 32.2 11
dbj|AB434304.1| Influenza A virus (A/swine/Chonburi/NIAH9469/... 32.2 11
dbj|AB434296.1| Influenza A virus (A/swine/Ratchaburi/NIAH550... 32.2 11
gb|EU798784.1| Influenza A virus (A/swine/Korea/Asan04/2006(H... 32.2 11
gb|EU798783.1| Influenza A virus (A/swine/Korea/JL04/2005(H1N... 32.2 11
gb|EU798781.1| Influenza A virus (A/swine/Korea/JL01/2005(H1N... 32.2 11
gb|EU798780.1| Influenza A virus (A/swine/Korea/Hongsong2/200... 32.2 11
gb|EU296605.1| Influenza A virus (A/swine/Chonburi/06CB2/2006... 32.2 11
gb|EU296603.1| Influenza A virus (A/swine/Chonburi/05CB1/2005... 32.2 11
gb|EU296601.1| Influenza A virus (A/swine/Chachoengsao/NIAH58... 32.2 11
gb|EU296599.1| Influenza A virus (A/swine/Chonburi/NIAH589/20... 32.2 11
gb|CY026427.1| Influenza A virus (A/swine/Jamesburg/1942(H1N1... 32.2 11
gb|CY022469.1| Influenza A virus (A/swine/Kansas/3228/1987(H1... 32.2 11
gb|EF101749.1| Influenza A virus (A/Thailand/271/2005(H1N1)) ... 32.2 11
gb|DQ431990.1| Influenza A virus (A/swine/Taiwan/CO935/2004(H... 32.2 11
gb|DQ447187.1| Influenza A virus (A/swine/Taiwan/CO935/2004(H... 32.2 11
gb|AY852271.1| Influenza A virus (A/swine/Guangdong/711/2001(... 32.2 11
gb|U80950.1|IAU80950 Influenza A virus hemagglutinin (HA) gen... 32.2 11
gb|U72666.1|IAU72666 Influenza A virus hemagglutinin (HA) gen... 32.2 11
 
Re: Denmark Tamiflu Resistant Sequence Released

NJ isolate above is from Fort Dix (1976 swine flu outbreak).
 
Re: Denmark Tamiflu Resistant Sequence Released

PB2 travel log

gb|GQ160588.1| Influenza A virus (A/Virginia/04/2009(H1N1)) s... 38.2 0.19
 
Re: Denmark Tamiflu Resistant Sequence Released

PB1 travel log

dbj|AB441949.1| Influenza A virus (A/Sendai-H/271/2007(H3N2))... 32.2 11
dbj|AB441947.1| Influenza A virus (A/Sendai-H/F150/2007(H3N2)... 32.2 11
gb|CY031569.1| Influenza A virus (A/California/UR06-0588/2007... 32.2 11
gb|CY030211.1| Influenza A virus (A/Virginia/UR06-0605/2007(H... 32.2 11
gb|CY030203.1| Influenza A virus (A/Illinois/UR06-0600/2007(H... 32.2 11
gb|CY026201.1| Influenza A virus (A/California/UR06-0589/2007... 32.2 11
gb|CY025865.1| Influenza A virus (A/Alabama/UR06-0545/2007(H3... 32.2 11
gb|CY025841.1| Influenza A virus (A/New York/UR06-0373/2007(H... 32.2 11
 
Re: Denmark Tamiflu Resistant Sequence Released

NP travel log

dbj|AB268554.2| Influenza A virus (A/duck/Mongolia/47/2001(H7... 40.1 0.047
gb|CY036158.1| Influenza A virus (A/long-tailed shrike/Hong K... 40.1 0.047
gb|CY031159.1| Influenza A virus (A/gray teal/Western Austral... 40.1 0.047
gb|CY024789.1| Influenza A virus (A/chicken/Victoria/1976(H7N... 40.1 0.047
gb|CY005727.1| Influenza A virus (A/wedge-tailed shearwater/W... 40.1 0.047
gb|CY005720.1| Influenza A virus (A/sooty tern/Western Austra... 40.1 0.047
gb|CY005408.1| Influenza A virus (A/wedge-tailed shearwater/W... 40.1 0.047
gb|CY005742.1| Influenza A virus (A/duck/NZL/164/1976(H11N3))... 40.1 0.047
gb|CY005737.1| Influenza A virus (A/duck/NZL/160/1976(H1N3)) ... 40.1 0.047
gb|CY005694.1| Influenza A virus (A/black duck/AUS/4045/1980(... 40.1 0.047
gb|CY005682.1| Influenza A virus (A/gray teal/AUS/3/1979(H4N6... 40.1 0.047
gb|CY005674.1| Influenza A virus (A/grey teal/Australia/2/197... 40.1 0.047
gb|AY623834.1| Influenza A virus (A/swine/Pingtung/199-2/2002... 40.1 0.047
gb|M30761.1|FLANPS Influenza A virus (A/grey teal/Australia/2... 40.1 0.047
 
Re: Denmark Tamiflu Resistant Sequence Released

niman said:
PB1 travel log

dbj|AB441949.1| Influenza A virus (A/Sendai-H/271/2007(H3N2))... 32.2 11
dbj|AB441947.1| Influenza A virus (A/Sendai-H/F150/2007(H3N2)... 32.2 11
gb|CY031569.1| Influenza A virus (A/California/UR06-0588/2007... 32.2 11
gb|CY030211.1| Influenza A virus (A/Virginia/UR06-0605/2007(H... 32.2 11
gb|CY030203.1| Influenza A virus (A/Illinois/UR06-0600/2007(H... 32.2 11
gb|CY026201.1| Influenza A virus (A/California/UR06-0589/2007... 32.2 11
gb|CY025865.1| Influenza A virus (A/Alabama/UR06-0545/2007(H3... 32.2 11
gb|CY025841.1| Influenza A virus (A/New York/UR06-0373/2007(H... 32.2 11
Click to expand...
It is worth noting that PB1 in pandemic swine H1N1 is human H3N2 from 1993. The travel log is of human H3N2 from 2007.

This is NOT a coincidence and NOT due to "random mutation".
 
Re: Denmark Tamiflu Resistant Sequence Released

Niman could you please post a short summary for us who don?t understand this kind of information? meaning, what does it mean and what is the consequenses? is this a mutation we should be worried about?

thanks

Theforeigner
 
Re: Denmark Tamiflu Resistant Sequence Released

NS travel log

gb|GQ160590.1| Influenza A virus (A/Virginia/04/2009(H1N1)) s... 38.2 0.19
 
Re: Denmark Tamiflu Resistant Sequence Released

theforeigner said:
Niman could you please post a short summary for us who don?t understand this kind of information? meaning, what does it mean and what is the consequenses? is this a mutation we should be worried about?

thanks

Theforeigner
Click to expand...
The PB1 travel log is the only newly acquired polymorphism, A178T, in the PB1 sequence. I suspect it is nonsynomous because it is a transversion (swaps a purine for a pyrimidine) but haven't looked yet. I believe the donor sequences is Brisbane/10 (but also haven't looked yet). The polymorphisms represents an "upgrade" so to speak (adding a human 2007 polymorphism to a 1993 sequence).
 
Re: Denmark Tamiflu Resistant Sequence Released

Denmark has also released two companion sequences

A/Denmark/523/2009

A/Denmark/524/2009
 
Re: Denmark Tamiflu Resistant Sequence Released

LOCUS CY043344 1422 bp cRNA linear VRL 04-AUG-2009
DEFINITION Influenza A virus (A/Denmark/524/2009(H1N1)) segment 6 sequence.
ACCESSION CY043344
VERSION CY043344.1 GI:255103474
DBLINK Project:37813
KEYWORDS .
SOURCE Influenza A virus (A/Denmark/524/2009(H1N1))
ORGANISM Influenza A virus (A/Denmark/524/2009(H1N1))
Viruses; ssRNA negative-strand viruses; Orthomyxoviridae;
Influenzavirus A.
REFERENCE 1 (bases 1 to 1422)
AUTHORS Gregory,V., Nielson,L., Zheng,X., Whittaker,L., Daniels,R. and
Hay,A.
TITLE Pandemic influenza A(H1N1) virus sequences
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1422)
AUTHORS Gregory,V., Nielson,L., Zheng,X., Whittaker,L., Daniels,R. and
Hay,A.
TITLE Direct Submission
JOURNAL Submitted (04-AUG-2009) WHO Collaborating Centre for Reference and
Research on Influenza, National Institute for Medical Research, The
Ridgeway, Mill Hill, London NW7 1AA, UK
FEATURES Location/Qualifiers
source 1..1422
/organism="Influenza A virus (A/Denmark/524/2009(H1N1))"
/mol_type="viral cRNA"
/strain="A/Denmark/524/2009(H1N1)"
/serotype="H1N1"
/isolation_source="gender:Male; age:22y"
/host="human"
/db_xref="taxon:664180"
/segment="6"
/lab_host="swine kidney 2, MDCK 2 passage(s)"
/country="Denmark"
/collection_date="04-Jun-2009"
/note="Oseltamivir sensitive, Zanamivir sensitive,
Amantidine resistant (31N in M2), lineage: swl"
gene 1..1410
/gene="NA"
CDS 1..1410
/gene="NA"
/codon_start=1
/product="neuraminidase"
/protein_id="ACU00944.1"
/db_xref="GI:255103475"
/translation="MNPNQKIITIGSVCMTIGMANLILQIGNIISIWISHSIQLGNQN
QIETCNQSVITYENNTWVNQTYVNISNTNFAAGQSVVSVKLAGNSSLCPVSGWAIYSK
DNSIRIGSKGDVFVIREPFISCSPLECRTFFLTQGALLNDKHSNGTIKDRSPYRTLMS
CPIGEVPSPYNSRFESVAWSASACHDGINWLTIGISGPDNGAVAVLKYNGIITDTIKS
WRNNILRTQESECACVNGSCFTVMTDGPSDGQASYKIFRIEKGKIVKSVEMNAPNYHY
EECSCYPDSSEITCVCRDNWHGSNRPWVSFNQNLEYQIGYICSGIFGDNPRPNDKTGS
CGPVSSNGANGVKGFSFKYGNGVWIGRTKSISSRNGFEMIWDPNGWTGTDNNFSIKQD
IVGINEWSGYSGSFVQHPELTGLDCIRPCFWVELIRGRPKENTIWTSGSSISFCGVNS
DTVGWSWPDGAELPFTIDK"
ORIGIN
1 atgaatccaa accaaaagat aataaccatt ggttcggtct gtatgacaat tggaatggct
61 aacttaatat tacaaattgg aaacataatc tcaatatgga ttagccactc aattcaactt
121 gggaatcaaa atcagattga aacatgcaat caaagcgtca ttacttatga aaacaacact
181 tgggtaaatc agacatatgt taacatcagc aacaccaact ttgctgctgg acagtcagtg
241 gtttccgtga aattagcggg caattcctct ctctgccctg ttagtggatg ggctatatac
301 agtaaagaca acagtataag aatcggttcc aagggggatg tgtttgtcat aagggaacca
361 ttcatatcat gctccccctt ggaatgcaga accttcttct tgactcaagg ggccttgcta
421 aatgacaaac attccaatgg aaccattaaa gacaggagcc catatcgaac cctaatgagc
481 tgtcctattg gtgaagttcc ctctccatac aactcaagat ttgagtcagt cgcttggtca
541 gcaagtgctt gtcatgatgg catcaattgg ctaacaattg gaatttctgg cccagacaat
601 ggggcagtgg ctgtgttaaa gtacaacggc ataataacag acactatcaa gagttggaga
661 aacaatatat tgagaacaca agagtctgaa tgtgcatgtg taaatggttc ttgctttact
721 gtaatgaccg atggaccaag tgatggacag gcctcataca agatcttcag aatagaaaag
781 ggaaagatag tcaaatcagt cgaaatgaat gcccctaatt atcactatga ggaatgctcc
841 tgttatcctg attctagtga aatcacatgt gtgtgcaggg ataactggca tggctcgaat
901 cgaccgtggg tgtctttcaa ccagaatctg gaatatcaga taggatacat atgcagtggg
961 attttcggag acaatccacg ccctaatgat aagacaggca gttgtggtcc agtatcgtct
1021 aatggagcaa atggagtaaa aggattttca ttcaaatacg gcaatggtgt ttggataggg
1081 agaactaaaa gcattagttc aagaaacggt tttgagatga tttgggatcc gaacggatgg
1141 actgggacag acaataactt ctcaataaag caagatatcg taggaataaa tgagtggtca
1201 ggatatagcg ggagttttgt tcagcatcca gaactaacag ggctggattg tataagacct
1261 tgcttctggg ttgaactaat cagagggcga cccaaagaga acacaatctg gactagcggg
1321 agcagcatat ccttttgtgg tgtaaacagt gacactgtgg gttggtcttg gccagacggt
1381 gctgagttgc catttaccat tgacaagtaa tttgttcaaa aa
 
Re: Denmark Tamiflu Resistant Sequence Released

LOCUS CY043336 1423 bp cRNA linear VRL 04-AUG-2009
DEFINITION Influenza A virus (A/Denmark/523/2009(H1N1)) segment 6 sequence.
ACCESSION CY043336
VERSION CY043336.1 GI:255103456
DBLINK Project:37813
KEYWORDS .
SOURCE Influenza A virus (A/Denmark/523/2009(H1N1))
ORGANISM Influenza A virus (A/Denmark/523/2009(H1N1))
Viruses; ssRNA negative-strand viruses; Orthomyxoviridae;
Influenzavirus A.
REFERENCE 1 (bases 1 to 1423)
AUTHORS Gregory,V., Nielson,L., Zheng,X., Whittaker,L., Daniels,R. and
Hay,A.
TITLE Pandemic influenza A(H1N1) virus sequences
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1423)
AUTHORS Gregory,V., Nielson,L., Zheng,X., Whittaker,L., Daniels,R. and
Hay,A.
TITLE Direct Submission
JOURNAL Submitted (04-AUG-2009) WHO Collaborating Centre for Reference and
Research on Influenza, National Institute for Medical Research, The
Ridgeway, Mill Hill, London NW7 1AA, UK
FEATURES Location/Qualifiers
source 1..1423
/organism="Influenza A virus (A/Denmark/523/2009(H1N1))"
/mol_type="viral cRNA"
/strain="A/Denmark/523/2009(H1N1)"
/serotype="H1N1"
/isolation_source="gender:Female; age:11y"
/host="human"
/db_xref="taxon:664179"
/segment="6"
/lab_host="swine kidney 2, MDCK 2 passage(s)"
/country="Denmark"
/collection_date="04-Jun-2009"
/note="Oseltamivir sensitive, Zanamivir sensitive,
Amantidine resistant (31N in M2), lineage: swl"
gene 1..1410
/gene="NA"
CDS 1..1410
/gene="NA"
/codon_start=1
/product="neuraminidase"
/protein_id="ACU00934.1"
/db_xref="GI:255103457"
/translation="MNPNQKIITIGSVCMTIGMANLILQIGNIISIWISHSIQLGNQN
QIETCNQSVITYENNTWVNQTYVNISNTNFAAGQSVVSVKLAGNSSLCPVSGWAIYSK
DNSIRIGSKGDVFVIREPFISCSPLECRTFFLTQGALLNDKHSNGTIKDRSPYRTLMS
CPIGEVPSPYNSRFESVAWSASACHDGINWLTIGISGPDNGAVAVLKYNGIITDTIKS
WRNNILRTQESECACVNGSCFTVMTDGPSDGQASYKIFRIEKGKIVKSVEMNAPNYHY
EECSCYPDSSEITCVCRDNWHGSNRPWVSFNQNLEYQIGYICSGIFGDNPRPNDKTGS
CGPVSSNGANGVKGFSFKYGNGVWIGRTKSISSRNGFEMIWDPNGWTGTDNNFSIKQD
IVGINEWSGYSGSFVQHPELTGLDCIRPCFWVELIRGRPKENTIWTSGSSISFCGVNS
DTVGWSWPDGAELPFTIDK"
ORIGIN
1 atgaatccaa accaaaagat aataaccatt ggttcggtct gtatgacaat tggaatggct
61 aacttaatat tacaaattgg aaacataatc tcaatatgga ttagccactc aattcaactt
121 gggaatcaaa atcagattga aacatgcaat caaagcgtca ttacttatga aaacaacact
181 tgggtaaatc agacatatgt taacatcagc aacaccaact ttgctgctgg acagtcagtg
241 gtttccgtga aattagcggg caattcctct ctctgccctg ttagtggatg ggctatatac
301 agtaaagaca acagtataag aatcggttcc aagggggatg tgtttgtcat aagggaacca
361 ttcatatcat gctccccctt ggaatgcaga accttcttct tgactcaagg ggccttgcta
421 aatgacaaac attccaatgg aaccattaaa gacaggagcc catatcgaac cctaatgagc
481 tgtcctattg gtgaagttcc ctctccatac aactcaagat ttgagtcagt cgcttggtca
541 gcaagtgctt gtcatgatgg catcaattgg ctaacaattg gaatttctgg cccagacaat
601 ggggcagtgg ctgtgttaaa gtacaacggc ataataacag acactatcaa gagttggaga
661 aacaatatat tgagaacaca agagtctgaa tgtgcatgtg taaatggttc ttgctttact
721 gtaatgaccg atggaccaag tgatggacag gcctcataca agatcttcag aatagaaaag
781 ggaaagatag tcaaatcagt cgaaatgaat gcccctaatt atcactatga ggaatgctcc
841 tgttatcctg attctagtga aatcacatgt gtgtgcaggg ataactggca tggctcgaat
901 cgaccgtggg tgtctttcaa ccagaatctg gaatatcaga taggatacat atgcagtggg
961 attttcggag acaatccacg ccctaatgat aagacaggca gttgtggtcc agtatcgtct
1021 aatggagcaa atggagtaaa aggattttca ttcaaatacg gcaatggtgt ttggataggg
1081 agaactaaaa gcattagttc aagaaacggt tttgagatga tttgggatcc gaacggatgg
1141 actgggacag acaataactt ctcaataaag caagatatcg taggaataaa tgagtggtca
1201 ggatatagcg ggagttttgt tcagcatcca gaactaacag ggctggattg tataagacct
1261 tgcttctggg ttgaactaat cagagggcga cccaaagaga acacaatctg gactagcggg
1321 agcagcatat ccttttgtgg tgtaaacagt gacactgtgg gttggtcttg gccagacggt
1381 gctgagttgc catttaccat tgacaagtaa tttgttcaaa aaa
 
Re: Denmark Tamiflu Resistant Sequence Released

The two companion sequences were collected Jun 4 (11F and 24M). The patient (21F) with resistance developed symptoms 5 days after the start of prophylactic Tamifu. The sample from her was collected 5 days after (Jun 9) the companion samples.
 
Re: Denmark Tamiflu Resistant Sequence Released

niman said:
The PB1 travel log is the only newly acquired polymorphism, A178T, in the PB1 sequence. I suspect it is nonsynomous because it is a transversion (swaps a purine for a pyrimidine) but haven't looked yet. I believe the donor sequences is Brisbane/10 (but also haven't looked yet). The polymorphisms represents an "upgrade" so to speak (adding a human 2007 polymorphism to a 1993 sequence).
Click to expand...

Thanks Niman, again, for trying to explain.
However it is still an explanation that the average person dont understand at all (myself included).
I know that som danish journalists and county politicians ect read this danish thread and I wish you could post an explanation that they have a chance to understnad.

Thanks

Tf
 
Re: Denmark Tamiflu Resistant Sequence Released

theforeigner said:
Thanks Niman, again, for trying to explain.
However it is still an explanation that the average person dont understand at all (myself included).
I know that som danish journalists and county politicians ect read this danish thread and I wish you could post an explanation that they have a chance to understnad.

Thanks

Tf
Click to expand...
I am still going through the sequences. The NA sequences for the other two isolates have no new changes, so the only difference between the three NA sequences is the H274Y (Tamiflu resistance) in the patient on prophylactic Tamiflu (confirming earlier media reports).
 
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