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Protein Pept Lett
. 2022 Dec 6.
doi: 10.2174/0929866530666221206094926. Online ahead of print.
ACE2 receptor Polymorphism and its correlation with anti-SARS-CoV-2 IgM Antibodies - a case-control study
Anum Siraj 1 , Saira Yahya 1 , Amber Khan 1 , Saleem Awan 1
Affiliations
- PMID: 36475331
- DOI: 10.2174/0929866530666221206094926
Abstract
Background: It has been reported that the SARS-CoV-2 pandemic originated in Wuhan, China in December 2019 and spread rapidly worldwide. The virus gets entry into target cells via angiotensin-converting enzyme 2 (ACE2) receptors and its gene is highly polymorphic.
Introduction: the variations in SARS-CoV-2 susceptibility and severity can be explained on a genetic level by studying the polymorphism in ACE2 receptor polymorphism.
Objective: A prospective case-control study was designed to compare the ACE2 levels in SARS-CoV-2 patients with the healthy controls in the local population, for which a total of 100 EDTA-containing blood samples were included (50 SARS-CoV-2 IgM positive case and 50 healthy controls).
Methods: PCR-RFLP was performed to investigate the polymorphism of ACE2 in genomic DNA and the ACE2 plasma levels were determined through ELISA.
Results: No significant difference in allelic and genotype frequencies (GG, GA, AA) were observed while the ACE2 plasma levels were found to be decreased in positive samples.
Conclusion: No significant association of the ACE2 gene polymorphism (G8790A) was found with the SARS-CoV-2 susceptibility in the Pakistani population which intimates the search for other genetic factors within the local population.
Keywords: Angiotensin-converting enzyme 2 (ACE2); Enzyme-linked immunosorbent assay (ELISA).; Renin-angiotensin system (RAS); Restriction fragment length polymorphism (RFLP); Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).