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Vaccination with whole inactivated virus vaccine affects the induction of heterosubtypic immunity against influenza A/H5N1 and immunodominance of virus specific CD8+ T cell responses in mice. (J Gen Virol., abstract, edited)
[Source: US National Library of Medicine, (LINK). Edited.]
J Gen Virol. 2010 Mar 24. [Epub ahead of print]
Vaccination with whole inactivated virus vaccine affects the induction of heterosubtypic immunity against influenza A/H5N1 and immunodominance of virus specific CD8+ T cell responses in mice.
Bodewes R, Kreijtz JH, Hillaire M, Geelhoed-Mieras MM, Fouchier RA, Osterhaus AD, Rimmelzwaan GF. - Department of Virology, Erasmus Medical Center.
Recently we have demonstrated that the use of an experimental subunit vaccine protected mice against infection with a human A/H3N2 influenza virus, but consequently affected the induction of heterosubtypic immunity to a highly pathogenic A/H5N1 influenza virus, otherwise induced by the A/H3N2 infection. Since inactivated whole virus (WIV) vaccines are widely used to protect against seasonal influenza and also contain inner viral proteins like the nucleoprotein, we tested the potential of a WIV vaccine to induce protective immunity against infection with a homologous A/H3N2 (A/Hong Kong/2/68) and a heterosubtypic A/H5N1 influenza virus (A/Indonesia/5/05). As expected, the vaccine afforded protection against infection with the A/H3N2 virus only. In addition, we demonstrated that the use of WIV vaccine for the protection against A/H3N2 infection affected the induction of heterosubtypic immunity otherwise afforded by A/H3N2 influenza virus infection. The reduction of protective immunity correlated with changes in the immunodominance patterns of the CD8+ T cell responses directed to the epitopes located in the acid polymerase (PA224-233) and the nucleoprotein (NP366-374). In unvaccinated mice that experienced an infection with the A/H3N2 influenza virus, the magnitude of the CD8+ T cell response to both peptides was similar upon a secondary infection with an A/H5N1 influenza virus. In contrast, prior vaccination with WIV affected the immunodominance pattern and skewed the response after infection with influenza virus A/Indonesia/5/05 towards a dominant NP366-374 specific response. These findings may have implications for vaccination strategies aiming at the induction of protective immunity to seasonal and/or pandemic influenza.
PMID: 20335492 [PubMed - as supplied by publisher]
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[Source: US National Library of Medicine, (LINK). Edited.]
J Gen Virol. 2010 Mar 24. [Epub ahead of print]
Vaccination with whole inactivated virus vaccine affects the induction of heterosubtypic immunity against influenza A/H5N1 and immunodominance of virus specific CD8+ T cell responses in mice.
Bodewes R, Kreijtz JH, Hillaire M, Geelhoed-Mieras MM, Fouchier RA, Osterhaus AD, Rimmelzwaan GF. - Department of Virology, Erasmus Medical Center.
Recently we have demonstrated that the use of an experimental subunit vaccine protected mice against infection with a human A/H3N2 influenza virus, but consequently affected the induction of heterosubtypic immunity to a highly pathogenic A/H5N1 influenza virus, otherwise induced by the A/H3N2 infection. Since inactivated whole virus (WIV) vaccines are widely used to protect against seasonal influenza and also contain inner viral proteins like the nucleoprotein, we tested the potential of a WIV vaccine to induce protective immunity against infection with a homologous A/H3N2 (A/Hong Kong/2/68) and a heterosubtypic A/H5N1 influenza virus (A/Indonesia/5/05). As expected, the vaccine afforded protection against infection with the A/H3N2 virus only. In addition, we demonstrated that the use of WIV vaccine for the protection against A/H3N2 infection affected the induction of heterosubtypic immunity otherwise afforded by A/H3N2 influenza virus infection. The reduction of protective immunity correlated with changes in the immunodominance patterns of the CD8+ T cell responses directed to the epitopes located in the acid polymerase (PA224-233) and the nucleoprotein (NP366-374). In unvaccinated mice that experienced an infection with the A/H3N2 influenza virus, the magnitude of the CD8+ T cell response to both peptides was similar upon a secondary infection with an A/H5N1 influenza virus. In contrast, prior vaccination with WIV affected the immunodominance pattern and skewed the response after infection with influenza virus A/Indonesia/5/05 towards a dominant NP366-374 specific response. These findings may have implications for vaccination strategies aiming at the induction of protective immunity to seasonal and/or pandemic influenza.
PMID: 20335492 [PubMed - as supplied by publisher]
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