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Clin Nutr. 2017 Jan 28. pii: S0261-5614(17)30035-3. doi: 10.1016/j.clnu.2017.01.011. [Epub ahead of print]
Impact of ageing and a synbiotic on the immune response to seasonal influenza vaccination; a randomised controlled trial.
Enani S1, Przemska-Kosicka A1, Childs CE1, Maidens C1, Dong H1, Conterno L2, Tuohy K2, Todd S3, Gosney M1, Yaqoob P4.
Author information
Abstract
BACKGROUND & AIMS:
Ageing increases risk of respiratory infections and impairs the response to influenza vaccination. Pre- and pro-biotics offer an opportunity to modulate anti-viral defenses and the response to vaccination via alteration of the gut microbiota. This study investigated the effect of a novel probiotic, Bifidobacterium longum bv. infantis CCUG 52486, combined with a prebiotic, gluco-oligosaccharide, on the B and T cell response to seasonal influenza vaccination in young and older subjects .
METHODS:
In a double-blind, randomized controlled trial, 58 young (18-35 y) and 54 older (60-85 y) subjects were supplemented with the synbiotic for 8 weeks. At 4 weeks they were administered with a seasonal influenza vaccine. B and T cell phenotype and responsiveness to in vitro re-stimulation with the vaccine were assessed at baseline, 4, 6 and 8 weeks.
RESULTS:
B and T cell profiles differed markedly between young and older subjects. Vaccination increased numbers of memory, IgA+ memory, IgG+ memory and total IgG+ B cells in young subjects, but failed to do so in older subjects and did not significantly alter T cell subsets. Seroconversion to the H1N1 subunit in the older subjects was associated with higher post-vaccination numbers of plasma B cells, but seroconversion was less consistently associated with T cell phenotype. B and T cell subsets from both young and older subjects demonstrated a strong antigen-specific recall challenge, and although not influenced by age, responsiveness to the recall challenge was associated with seroconversion. In older subjects, CMV seropositivity was associated with a significantly lower recall response to the vaccine, but the synbiotic did not affect the responsiveness of B or T cells to re-stimulation with influenza vaccine.
CONCLUSIONS:
Antigen-specific B and T cell activation following an in vitro recall challenge with the influenza vaccine was influenced by CMV seropositivity, but not by a synbiotic. Registered under ClinicalTrials.gov Identifier no. NCT01066377.
Copyright ? 2017 The Author(s). Published by Elsevier Ltd.. All rights reserved.
KEYWORDS:
Ageing; Influenza; Lymphocyte; Probiotic; Vaccination
PMID: 28215759 DOI: 10.1016/j.clnu.2017.01.011
[PubMed - as supplied by publisher]
Impact of ageing and a synbiotic on the immune response to seasonal influenza vaccination; a randomised controlled trial.
Enani S1, Przemska-Kosicka A1, Childs CE1, Maidens C1, Dong H1, Conterno L2, Tuohy K2, Todd S3, Gosney M1, Yaqoob P4.
Author information
Abstract
BACKGROUND & AIMS:
Ageing increases risk of respiratory infections and impairs the response to influenza vaccination. Pre- and pro-biotics offer an opportunity to modulate anti-viral defenses and the response to vaccination via alteration of the gut microbiota. This study investigated the effect of a novel probiotic, Bifidobacterium longum bv. infantis CCUG 52486, combined with a prebiotic, gluco-oligosaccharide, on the B and T cell response to seasonal influenza vaccination in young and older subjects .
METHODS:
In a double-blind, randomized controlled trial, 58 young (18-35 y) and 54 older (60-85 y) subjects were supplemented with the synbiotic for 8 weeks. At 4 weeks they were administered with a seasonal influenza vaccine. B and T cell phenotype and responsiveness to in vitro re-stimulation with the vaccine were assessed at baseline, 4, 6 and 8 weeks.
RESULTS:
B and T cell profiles differed markedly between young and older subjects. Vaccination increased numbers of memory, IgA+ memory, IgG+ memory and total IgG+ B cells in young subjects, but failed to do so in older subjects and did not significantly alter T cell subsets. Seroconversion to the H1N1 subunit in the older subjects was associated with higher post-vaccination numbers of plasma B cells, but seroconversion was less consistently associated with T cell phenotype. B and T cell subsets from both young and older subjects demonstrated a strong antigen-specific recall challenge, and although not influenced by age, responsiveness to the recall challenge was associated with seroconversion. In older subjects, CMV seropositivity was associated with a significantly lower recall response to the vaccine, but the synbiotic did not affect the responsiveness of B or T cells to re-stimulation with influenza vaccine.
CONCLUSIONS:
Antigen-specific B and T cell activation following an in vitro recall challenge with the influenza vaccine was influenced by CMV seropositivity, but not by a synbiotic. Registered under ClinicalTrials.gov Identifier no. NCT01066377.
Copyright ? 2017 The Author(s). Published by Elsevier Ltd.. All rights reserved.
KEYWORDS:
Ageing; Influenza; Lymphocyte; Probiotic; Vaccination
PMID: 28215759 DOI: 10.1016/j.clnu.2017.01.011
[PubMed - as supplied by publisher]