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Int J Biol Macromol. 2016 Jun 20. pii: S0141-8130(16)30595-5. doi: 10.1016/j.ijbiomac.2016.06.057. [Epub ahead of print]
Improvement of immune responses to influenza vaccine (H5N1) by sulfated yeast beta-glucan.
Wang M1, Zhang L2, Yang R2, Fei C2, Wang X2, Zhang K2, Wang C2, Zheng W2, Xue F3.
Author information
Abstract
In this study, the adjuvant activity of sulfated glucan from saccharomyces cerevisiae (SGSC) was investigated. sGSC had molecular weight of 12.9kDa and degrees of sulfation of 0.16.The effects of SGSC on the splenic lymphocyte cells of mice were measured in vitro. The results showed that SGSC could significantly promote lymphocyte proliferation singly or synergistically with Con A and LPS, and stimulate the cells to secrete IL-2 and INF-γ. The adjuvant activity of SGSC was researched in BALB/c mice inoculated with inactivated H5N1vaccine in vivo. The results showed that SGSC could significantly enhance lymphocyte proliferation, effectively increase the percentage of CD4+ T cells, decrease the percentage of CD8+ T cells, and elevate the CD4+/CD8+ ratio; enhance the HI antibody titre, and promote the production of IL-2, INF-γ, IL-4 and IL-6 at medium level. These results indicated that sulfated glucan showed an excellent adjuvant effect on H5N1vaccine in a mouse model. Therefore, SGSC could be used as an effective immune adjuvant for an inactivated H5N1vaccine.
Copyright ? 2016. Published by Elsevier B.V.
KEYWORDS:
Adjuvant activity; H5N1vaccine; Sulfated glucan from saccharomyces cerevisiae
PMID: 27339320 DOI: 10.1016/j.ijbiomac.2016.06.057
[PubMed - as supplied by publisher]
Improvement of immune responses to influenza vaccine (H5N1) by sulfated yeast beta-glucan.
Wang M1, Zhang L2, Yang R2, Fei C2, Wang X2, Zhang K2, Wang C2, Zheng W2, Xue F3.
Author information
Abstract
In this study, the adjuvant activity of sulfated glucan from saccharomyces cerevisiae (SGSC) was investigated. sGSC had molecular weight of 12.9kDa and degrees of sulfation of 0.16.The effects of SGSC on the splenic lymphocyte cells of mice were measured in vitro. The results showed that SGSC could significantly promote lymphocyte proliferation singly or synergistically with Con A and LPS, and stimulate the cells to secrete IL-2 and INF-γ. The adjuvant activity of SGSC was researched in BALB/c mice inoculated with inactivated H5N1vaccine in vivo. The results showed that SGSC could significantly enhance lymphocyte proliferation, effectively increase the percentage of CD4+ T cells, decrease the percentage of CD8+ T cells, and elevate the CD4+/CD8+ ratio; enhance the HI antibody titre, and promote the production of IL-2, INF-γ, IL-4 and IL-6 at medium level. These results indicated that sulfated glucan showed an excellent adjuvant effect on H5N1vaccine in a mouse model. Therefore, SGSC could be used as an effective immune adjuvant for an inactivated H5N1vaccine.
Copyright ? 2016. Published by Elsevier B.V.
KEYWORDS:
Adjuvant activity; H5N1vaccine; Sulfated glucan from saccharomyces cerevisiae
PMID: 27339320 DOI: 10.1016/j.ijbiomac.2016.06.057
[PubMed - as supplied by publisher]