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Biochem Biophys Res Commun. 2015 Oct 15. pii: S0006-291X(15)30767-1. doi: 10.1016/j.bbrc.2015.10.067. [Epub ahead of print]
Lactoferrin acts as an adjuvant during influenza vaccination of neonatal mice.
Sherman MP1, Pritzl CJ2, Xia C3, Miller MM4, Zaghouani H5, Hahm B6.
Author information
Abstract
Health policy precludes neonatal vaccination against influenza. Hence, morbidity and mortality are high under 6 months of age. Lactoferrin may activate diminished numbers of dysfunctional dendritic cells and reverse neonatal vaccine failures. Aluminum hydroxide/ALUM recruits neutrophils that secrete lactoferrin at deposition sites of antigen. We theorized lactoferrin + influenza antigen initiates an equivalent antibody response compared to ALUM. Three-day-old mice received subcutaneously 30 micrograms of H1N1 hemagglutinin + 200 μg of bovine lactoferrin versus hemagglutinin + ALUM. Controls received hemagglutinin, lactoferrin, or ALUM. After 21 days, sera measured anti-H1N1 (ELISA) and neutralizing antibody (plaque assays). ELISA detected equal antibody production with lactoferrin + hemagglutinin compared to hemagglutinin + ALUM; both sera also neutralized H1N1 virus at a 1:20 dilution (p<.01). Controls had no anti-H1N1 antibody. Neonates given lactoferrin had no anaphylaxis when challenged four weeks later. Lactoferrin is a safe and effective adjuvant for inducing antibody against influenza in neonates.
Copyright ? 2015. Published by Elsevier Inc.
KEYWORDS:
adjuvant; hemagglutinin; immunization; influenza; lactoferrin; newborn
PMID: 26478433 [PubMed - as supplied by publisher]
Lactoferrin acts as an adjuvant during influenza vaccination of neonatal mice.
Sherman MP1, Pritzl CJ2, Xia C3, Miller MM4, Zaghouani H5, Hahm B6.
Author information
Abstract
Health policy precludes neonatal vaccination against influenza. Hence, morbidity and mortality are high under 6 months of age. Lactoferrin may activate diminished numbers of dysfunctional dendritic cells and reverse neonatal vaccine failures. Aluminum hydroxide/ALUM recruits neutrophils that secrete lactoferrin at deposition sites of antigen. We theorized lactoferrin + influenza antigen initiates an equivalent antibody response compared to ALUM. Three-day-old mice received subcutaneously 30 micrograms of H1N1 hemagglutinin + 200 μg of bovine lactoferrin versus hemagglutinin + ALUM. Controls received hemagglutinin, lactoferrin, or ALUM. After 21 days, sera measured anti-H1N1 (ELISA) and neutralizing antibody (plaque assays). ELISA detected equal antibody production with lactoferrin + hemagglutinin compared to hemagglutinin + ALUM; both sera also neutralized H1N1 virus at a 1:20 dilution (p<.01). Controls had no anti-H1N1 antibody. Neonates given lactoferrin had no anaphylaxis when challenged four weeks later. Lactoferrin is a safe and effective adjuvant for inducing antibody against influenza in neonates.
Copyright ? 2015. Published by Elsevier Inc.
KEYWORDS:
adjuvant; hemagglutinin; immunization; influenza; lactoferrin; newborn
PMID: 26478433 [PubMed - as supplied by publisher]