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J Virol. 2015 Apr 29. pii: JVI.00808-15. [Epub ahead of print]
Design and Structure of an Engineered Disulfide-Stabilized Influenza Hemagglutinin Trimer.
Lee PS1, Zhu X2, Yu W2, Wilson IA3.
Author information
Abstract
We engineered a disulfide-stabilized influenza hemagglutinin (HA) trimer, termed HA3-SS, by introducing cysteine residues in the HA stem to covalently bridge the three protomers. HA3-SS has increased thermostability compared to wild-type HA and binding of head- and stem-targeted antibodies is preserved; only minor structural changes are found in the vicinity of the additional disulfide. This platform has been applied to H1 and H3 HAs and provides prospects for design of intact, stabilized influenza HA immunogens.
Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
PMID: 25926650 [PubMed - as supplied by publisher]
Design and Structure of an Engineered Disulfide-Stabilized Influenza Hemagglutinin Trimer.
Lee PS1, Zhu X2, Yu W2, Wilson IA3.
Author information
Abstract
We engineered a disulfide-stabilized influenza hemagglutinin (HA) trimer, termed HA3-SS, by introducing cysteine residues in the HA stem to covalently bridge the three protomers. HA3-SS has increased thermostability compared to wild-type HA and binding of head- and stem-targeted antibodies is preserved; only minor structural changes are found in the vicinity of the additional disulfide. This platform has been applied to H1 and H3 HAs and provides prospects for design of intact, stabilized influenza HA immunogens.
Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
PMID: 25926650 [PubMed - as supplied by publisher]