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Expert Rev Vaccines. 2015 Mar 27:1-10. [Epub ahead of print]
Efficient vaccine against pandemic influenza: combining DNA vaccination and targeted delivery to MHC class II molecules.
Gr?deland G1, Bogen B.
Author information
Abstract
There are two major limitations to vaccine preparedness in the event of devastating influenza pandemics: the time needed to generate a vaccine and rapid generation of sufficient amounts. DNA vaccination could represent a solution to these problems, but efficacy needs to be enhanced. In a separate line of research, it has been established that targeting of vaccine molecules to antigen-presenting cells enhances immune responses. We have combined the two principles by constructing DNA vaccines that encode bivalent fusion proteins; these target hemagglutinin to MHC class II molecules on antigen-presenting cells. Such DNA vaccines rapidly induce hemagglutinin-specific antibodies and T cell responses in immunized mice. Responses are long-lasting and protect mice against challenge with influenza virus. In a pandemic situation, targeted DNA vaccines could be produced and tested within a month. The novel DNA vaccines could represent a solution to pandemic preparedness in the advent of novel influenza pandemics.
KEYWORDS:
APC targeting; DNA vaccine; MHC II molecules; antibodies; influenza; vaccine
PMID: 25818107 [PubMed - as supplied by publisher]
Efficient vaccine against pandemic influenza: combining DNA vaccination and targeted delivery to MHC class II molecules.
Gr?deland G1, Bogen B.
Author information
Abstract
There are two major limitations to vaccine preparedness in the event of devastating influenza pandemics: the time needed to generate a vaccine and rapid generation of sufficient amounts. DNA vaccination could represent a solution to these problems, but efficacy needs to be enhanced. In a separate line of research, it has been established that targeting of vaccine molecules to antigen-presenting cells enhances immune responses. We have combined the two principles by constructing DNA vaccines that encode bivalent fusion proteins; these target hemagglutinin to MHC class II molecules on antigen-presenting cells. Such DNA vaccines rapidly induce hemagglutinin-specific antibodies and T cell responses in immunized mice. Responses are long-lasting and protect mice against challenge with influenza virus. In a pandemic situation, targeted DNA vaccines could be produced and tested within a month. The novel DNA vaccines could represent a solution to pandemic preparedness in the advent of novel influenza pandemics.
KEYWORDS:
APC targeting; DNA vaccine; MHC II molecules; antibodies; influenza; vaccine
PMID: 25818107 [PubMed - as supplied by publisher]