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Vaccine. 2014 Sep 17. pii: S0264-410X(14)01247-X. doi: 10.1016/j.vaccine.2014.08.079. [Epub ahead of print]
Human antibody response to N-glycans present on plant-made influenza virus-like particle (VLP) vaccines.
Ward BJ1, Landry N2, Tr?panier S3, Mercier G4, Dargis M5, Couture M6, D'Aoust MA7, V?zina LP8.
Author information
Abstract
BACKGROUND:
Plant-made biotherapeutics are gathering momentum and some plant glycoproteins are allergens. Glycans with core β1-2xylose and α1,3fucose motifs and antennae terminated by mannose residues (e.g.: MMXF) are found on several plant allergens and can cross-react with glyco-epitopes from other sources. To date, reactivity to these cross-reactive determinants has not been associated with clinical symptoms.
OBJECTIVE:
We produced VLP vaccines bearing the hemagglutinin(HA) of H5(A/Indonesia/5/05) or H1(A/California/07/09) influenza viruses by transfection of Nicotiana benthamiana. Subjects enrolled in Phase I/II trials were followed for evidence of allergy/hypersensitivity and development of antibodies against plant glyco-epitopes.
METHODS:
A total of 280/349 subjects received either one (H1) or 2 doses (H5) of vaccine (5-45μg of HA/dose) intramuscularly including 40 with pre-existing plant allergies. Subjects were monitored for 6 months. IgG and IgE to plant glyco-epitopes were measured by ELISA using corn-/egg-derived avidin and bromelain as target antigens.
RESULTS:
No subject developed allergic/hypersensitivity symptoms. Some (34%) developed transient IgG and, in some cases IgE, to plant glyco-epitopes but no subject mounted an IgE response to the MMXF motif. Antibodies returned to baseline by 6 months in most subjects.
CONCLUSION:
VLP vaccines bearing influenza HA glycoproteins can elicit transient IgG and, in some cases, IgE responses that are not associated with either the development or worsening of allergic/hypersensitivity symptoms.
Copyright ? 2014. Published by Elsevier Ltd.
KEYWORDS:
Allergy; Bromelain assay; CCDs; Cross-reactive carbohydrate determinants; Humoral response; IgE; IgG; Influenza A virus; Plant-made vaccines; Plant-specific glycans
PMID:
25240757
[PubMed - as supplied by publisher]
Human antibody response to N-glycans present on plant-made influenza virus-like particle (VLP) vaccines.
Ward BJ1, Landry N2, Tr?panier S3, Mercier G4, Dargis M5, Couture M6, D'Aoust MA7, V?zina LP8.
Author information
Abstract
BACKGROUND:
Plant-made biotherapeutics are gathering momentum and some plant glycoproteins are allergens. Glycans with core β1-2xylose and α1,3fucose motifs and antennae terminated by mannose residues (e.g.: MMXF) are found on several plant allergens and can cross-react with glyco-epitopes from other sources. To date, reactivity to these cross-reactive determinants has not been associated with clinical symptoms.
OBJECTIVE:
We produced VLP vaccines bearing the hemagglutinin(HA) of H5(A/Indonesia/5/05) or H1(A/California/07/09) influenza viruses by transfection of Nicotiana benthamiana. Subjects enrolled in Phase I/II trials were followed for evidence of allergy/hypersensitivity and development of antibodies against plant glyco-epitopes.
METHODS:
A total of 280/349 subjects received either one (H1) or 2 doses (H5) of vaccine (5-45μg of HA/dose) intramuscularly including 40 with pre-existing plant allergies. Subjects were monitored for 6 months. IgG and IgE to plant glyco-epitopes were measured by ELISA using corn-/egg-derived avidin and bromelain as target antigens.
RESULTS:
No subject developed allergic/hypersensitivity symptoms. Some (34%) developed transient IgG and, in some cases IgE, to plant glyco-epitopes but no subject mounted an IgE response to the MMXF motif. Antibodies returned to baseline by 6 months in most subjects.
CONCLUSION:
VLP vaccines bearing influenza HA glycoproteins can elicit transient IgG and, in some cases, IgE responses that are not associated with either the development or worsening of allergic/hypersensitivity symptoms.
Copyright ? 2014. Published by Elsevier Ltd.
KEYWORDS:
Allergy; Bromelain assay; CCDs; Cross-reactive carbohydrate determinants; Humoral response; IgE; IgG; Influenza A virus; Plant-made vaccines; Plant-specific glycans
PMID:
25240757
[PubMed - as supplied by publisher]
