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J Gen Virol. 2014 Jul 22. pii: vir.0.067488-0. doi: 10.1099/vir.0.067488-0. [Epub ahead of print]
Neuraminidase Gene Homology Contributes to the Protective Activity of Influenza Vaccines Prepared from the Influenza Virus Library.
Haredy AM1, Yamada H2, Sakoda Y3, Okamatsu M3, Yamamoto N3, Omasa T4, Mori Y2, Kida H3, Okamoto S2, Okuno Y5, Yamanishi K2.
Author information
Abstract
Whole virus (WV) vaccines from influenza A/duck/Hokkaido/77 (H3N2) and its reassortant strains H3N4, H3N5, H3N7, which have the same hemagglutinin (HA) gene but different neuraminidase (NA) genes, were prepared from our influenza virus library. Mice were intranasally immunized with equivalent concentrations of each vaccine (1 to 0.01 ?g/mouse). All of the mice that received the highest concentration of each vaccine (1 ?g/mouse) showed equivalent high hemagglutinin inhibition (HI) antibody titers of over 1:40, and survived the H3N2 challenge viruses. However, mice that received lower concentrations of vaccine (0.1 or 0.01 ?g/mouse) containing a heterologous NA had lower survival rates than those given the H3N2-based vaccine. The lungs of mice challenged with H3N2 virus showed a significantly higher virus clearance rate when the vaccine contained the homologous NA (N2) versus a heterologous NA, suggesting that NA contributes to the protection, especially when the HI antibody level is low. These results suggest that, even if vaccines prepared for a possible upcoming pandemic do not induce sufficient HI antibodies, WV vaccines can still be effective through other matched proteins such as NA.
Copyright ? 2014, the Society for General Microbiology.
KEYWORDS:
Neuraminidase inhibition antibody; heterologous NA; influenza virus library; intranasal immunization
PMID:
25053564
[PubMed - as supplied by publisher]
Neuraminidase Gene Homology Contributes to the Protective Activity of Influenza Vaccines Prepared from the Influenza Virus Library.
Haredy AM1, Yamada H2, Sakoda Y3, Okamatsu M3, Yamamoto N3, Omasa T4, Mori Y2, Kida H3, Okamoto S2, Okuno Y5, Yamanishi K2.
Author information
Abstract
Whole virus (WV) vaccines from influenza A/duck/Hokkaido/77 (H3N2) and its reassortant strains H3N4, H3N5, H3N7, which have the same hemagglutinin (HA) gene but different neuraminidase (NA) genes, were prepared from our influenza virus library. Mice were intranasally immunized with equivalent concentrations of each vaccine (1 to 0.01 ?g/mouse). All of the mice that received the highest concentration of each vaccine (1 ?g/mouse) showed equivalent high hemagglutinin inhibition (HI) antibody titers of over 1:40, and survived the H3N2 challenge viruses. However, mice that received lower concentrations of vaccine (0.1 or 0.01 ?g/mouse) containing a heterologous NA had lower survival rates than those given the H3N2-based vaccine. The lungs of mice challenged with H3N2 virus showed a significantly higher virus clearance rate when the vaccine contained the homologous NA (N2) versus a heterologous NA, suggesting that NA contributes to the protection, especially when the HI antibody level is low. These results suggest that, even if vaccines prepared for a possible upcoming pandemic do not induce sufficient HI antibodies, WV vaccines can still be effective through other matched proteins such as NA.
Copyright ? 2014, the Society for General Microbiology.
KEYWORDS:
Neuraminidase inhibition antibody; heterologous NA; influenza virus library; intranasal immunization
PMID:
25053564
[PubMed - as supplied by publisher]
