Cell Rep
. 2022 Jun 13;111013.
doi: 10.1016/j.celrep.2022.111013. Online ahead of print.
Temporal associations of B and T cell immunity with robust vaccine responsiveness in a 16-week interval BNT162b2 regimen
Manon Nayrac 1 , Mathieu Dubé 2 , Gérémy Sannier 1 , Alexandre Nicolas 1 , Lorie Marchitto 1 , Olivier Tastet 2 , Alexandra Tauzin 1 , Nathalie Brassard 2 , Raphaël Lima-Barbosa 3 , Guillaume Beaudoin-Bussières 1 , Dani Vézina 2 , Shang Yu Gong 4 , Mehdi Benlarbi 2 , Romain Gasser 1 , Annemarie Laumaea 1 , Jérémie Prévost 1 , Catherine Bourassa 2 , Gabrielle Gendron-Lepage 2 , Halima Medjahed 2 , Guillaume Goyette 2 , Gloria-Gabrielle Ortega-Delgado 2 , Mélanie Laporte 2 , Julia Niessl 1 , Laurie Gokool 2 , Chantal Morrisseau 2 , Pascale Arlotto 2 , Jonathan Richard 1 , Justin Bélair 3 , Alexandre Prat 5 , Cécile Tremblay 1 , Valérie Martel-Laferrière 1 , Andrés Finzi 6 , Daniel E Kaufmann 7
Affiliations
- PMID: 35732172
- DOI: 10.1016/j.celrep.2022.111013
Abstract
Spacing of BNT162b2 mRNA doses beyond 3 weeks raises concerns about vaccine efficacy. We longitudinally analyze B cell, T cell, and humoral responses to two BNT162b2 mRNA doses administered 16 weeks apart in 53 SARS-CoV-2 naive and previously infected donors. This regimen elicits robust RBD-specific B cell responses whose kinetics differs between cohorts, the second dose leading to increased magnitude in naive participants only. While boosting does not increase magnitude of CD4+ T cell responses further compared with the first dose, unsupervised clustering of single-cell features reveals phenotypic and functional shifts over time and between cohorts. Integrated analysis shows longitudinal immune component-specific associations, with early T helper responses post first dose correlating with B cell responses after the second dose, and memory T helper generated between doses correlating with CD8 T cell responses after boosting. Therefore, boosting elicits a robust cellular recall response after the 16-week interval, indicating functional immune memory.
Keywords: AIM assay; B cells; CD4 T cell; CD4 T cell help; CD8 T cell; COVID-19; CP: Immunology; SARS-CoV-2; Spike glycoproteins; coronavirus; humoral responses; immunological memory; longer interval; mRNA vaccine; vaccine regimen.