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Chembiochem. 2019 May 20. doi: 10.1002/cbic.201900331. [Epub ahead of print]
Strong binding and off-on signaling functions of deep-red fluorescent TO-PRO-3 for influenza A virus RNA promoter region.
Nishizawa S1, Sato Y2, Aiba Y2, Yajima S2, Tanabe T2, Higuchi K2.
Author information
Abstract
Influenza A virus RNA promoter region has recently attracted much attention as RNA targets for the development of novel anti-influenza drugs. However, there are very limited reports on small RNA-binding ligands targeting this region. In this work, we report that TO-PRO-3, a thiazole orange analogue with a trimethine bridge, exhibits the strong and selective binding to the internal loop structure of the influenza A virus RNA promoter. This binding accompanies the remarkable light-up response of TO-PRO-3 in the deep-red spectral region. By virtue of these binding and fluorescence signaling functions, TO-PRO-3 can work as a useful indicator for the assessment of the binding capabilities of various test compounds for this RNA region with a view toward the development of anti-influenza drug candidates.
? 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
KEYWORDS:
influenza A virus RNA promoter・anti-influenza drug・deep-red probe・fluorescence indicator・screening
PMID: 31106464 DOI: 10.1002/cbic.201900331
Strong binding and off-on signaling functions of deep-red fluorescent TO-PRO-3 for influenza A virus RNA promoter region.
Nishizawa S1, Sato Y2, Aiba Y2, Yajima S2, Tanabe T2, Higuchi K2.
Author information
Abstract
Influenza A virus RNA promoter region has recently attracted much attention as RNA targets for the development of novel anti-influenza drugs. However, there are very limited reports on small RNA-binding ligands targeting this region. In this work, we report that TO-PRO-3, a thiazole orange analogue with a trimethine bridge, exhibits the strong and selective binding to the internal loop structure of the influenza A virus RNA promoter. This binding accompanies the remarkable light-up response of TO-PRO-3 in the deep-red spectral region. By virtue of these binding and fluorescence signaling functions, TO-PRO-3 can work as a useful indicator for the assessment of the binding capabilities of various test compounds for this RNA region with a view toward the development of anti-influenza drug candidates.
? 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
KEYWORDS:
influenza A virus RNA promoter・anti-influenza drug・deep-red probe・fluorescence indicator・screening
PMID: 31106464 DOI: 10.1002/cbic.201900331