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J Tradit Chin Med. 2012 Dec;32(4):657-63.
An extract from the earthworm Eisenia fetida non-specifically inhibits the activity of influenza and adenoviruses.
Liu Z, Wang J, Zhang J, Yu B, Niu B.
Source
Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, Shanxi 030001, China.
Abstract
OBJECTIVE:
To test the in vitro antiviral activity of a crude tissue extract (CTE) from the earthworm Eisenia fetida, determine any effective components in the CTE, and elucidate possible mechanisms of action.
METHODS:
A CTE was made by homogenizing earthworms, followed by treatment with ammonium sulfate, then thermal denaturation. Inhibition of virus-induced cytopathic effect (CPE) was used to assess antiviral activity. Chromatographic analysis was used to identify effective components in the CTE.
RESULTS:
The CTE inhibited viral CPE at non-cytotoxic concentrations. Chromatography indicated that antiviral components corresponded to three active peaks indicative of proteases, nucleases and lysozymes. For adenoviruses, reduction in viral activity occurred for 100 microg/mL CTE. The reduction in adenoviral activity for four fractions was 100%, 91.8%, 86.9%, and 94.7%. For influenza viruses, reduction in viral activity of 100%, 86.6%, 69.1% and 88.3% was observed for 37 microg/mL CTE. In addition, three active fractions mixture had stronger antiviral activity (98.7% and 96.7%) than three fractions alone. Gel electrophoresis results indicated that nucleases from E. fetida could degrade the genome of influenza viruses and adenoviruses.
CONCLUSION:
The earthworm CTE displayed non-specific antiviral properties, possibly mediated by a combination of proteases, nucleases and lysozymes. Nucleases likely participate in the antiviral process, and degrade the genome of the virus thereby preventing further replication.
PMID:
23427406
[PubMed - in process]
An extract from the earthworm Eisenia fetida non-specifically inhibits the activity of influenza and adenoviruses.
Liu Z, Wang J, Zhang J, Yu B, Niu B.
Source
Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, Shanxi 030001, China.
Abstract
OBJECTIVE:
To test the in vitro antiviral activity of a crude tissue extract (CTE) from the earthworm Eisenia fetida, determine any effective components in the CTE, and elucidate possible mechanisms of action.
METHODS:
A CTE was made by homogenizing earthworms, followed by treatment with ammonium sulfate, then thermal denaturation. Inhibition of virus-induced cytopathic effect (CPE) was used to assess antiviral activity. Chromatographic analysis was used to identify effective components in the CTE.
RESULTS:
The CTE inhibited viral CPE at non-cytotoxic concentrations. Chromatography indicated that antiviral components corresponded to three active peaks indicative of proteases, nucleases and lysozymes. For adenoviruses, reduction in viral activity occurred for 100 microg/mL CTE. The reduction in adenoviral activity for four fractions was 100%, 91.8%, 86.9%, and 94.7%. For influenza viruses, reduction in viral activity of 100%, 86.6%, 69.1% and 88.3% was observed for 37 microg/mL CTE. In addition, three active fractions mixture had stronger antiviral activity (98.7% and 96.7%) than three fractions alone. Gel electrophoresis results indicated that nucleases from E. fetida could degrade the genome of influenza viruses and adenoviruses.
CONCLUSION:
The earthworm CTE displayed non-specific antiviral properties, possibly mediated by a combination of proteases, nucleases and lysozymes. Nucleases likely participate in the antiviral process, and degrade the genome of the virus thereby preventing further replication.
PMID:
23427406
[PubMed - in process]
