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Journal of Virology, September 2010, p. 8389-8397, Vol. 84, No. 17
0022-538X/10/$012.00+0 doi:10.1128/JVI.00413-10
Copyright ? 2010, American Society for Microbiology. All Rights Reserved
Yanbing Li,{ddagger} Jianzhong Shi,{ddagger} Gongxun Zhong,{ddagger} Guohua Deng,{ddagger} Guobin Tian, Jinying Ge, Xianying Zeng, Jiasheng Song, Dongming Zhao, Liling Liu, Yongping Jiang, Yuntao Guan, Zhigao Bu, and Hualan Chen*
Animal Influenza Laboratory of the Ministry of Agriculture and National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin 150001, People's Republic of China
Received 24 February 2010/ Accepted 28 May 2010
Despite substantial efforts to control H5N1 avian influenza viruses (AIVs), the viruses have continued to evolve and cause disease outbreaks in poultry and infections in humans. In this report, we analyzed 51 representative H5N1 AIVs isolated from domestic poultry, wild birds, and humans in China during 2004 to 2009, and 21 genotypes were detected based on whole-genome sequences. Twelve genotypes of AIVs in southern China bear similar H5 hemagglutinin (HA) genes (clade 2.3). These AIVs did not display antigenic drift and could be completely protected against by the A/goose/Guangdong/1/96 (GS/GD/1/96)-based oil-adjuvanted killed vaccine and recombinant Newcastle disease virus vaccine, which have been used in China. In addition, antigenically drifted H5N1 viruses, represented by A/chicken/Shanxi/2/06 (CK/SX/2/06), were detected in chickens from several provinces in northern China. The CK/SX/2/06-like viruses are reassortants with newly emerged HA, NA, and PB1 genes that could not be protected against by the GS/GD/1/96-based vaccines. These viruses also reacted poorly with antisera generated from clade 2.2 and 2.3 viruses. The majority of the viruses isolated from southern China were lethal in mice and ducks, while the CK/SX/2/06-like viruses caused mild disease in mice and could not replicate in ducks. Our results demonstrate that the H5N1 AIVs circulating in nature have complex biological characteristics and pose a continued challenge for disease control and pandemic preparedness.
* Corresponding author. Mailing address: Harbin Veterinary Research Institute, 427 Maduan Street, Harbin 150001, People's Republic of China. Phone: 86-451-82761925. Fax: 86-451-82733132. E-mail: hlchen1@yahoo.com
{triangledown} Published ahead of print on 10 June 2010.
{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.
{ddagger} Y.L., J.S., G.Z., and G.D. equally contributed to this paper.
Journal of Virology, September 2010, p. 8389-8397, Vol. 84, No. 17
0022-538X/10/$012.00+0 doi:10.1128/JVI.00413-10
Copyright ? 2010, American Society for Microbiology. All Rights Reserved.
0022-538X/10/$012.00+0 doi:10.1128/JVI.00413-10
Copyright ? 2010, American Society for Microbiology. All Rights Reserved
Yanbing Li,{ddagger} Jianzhong Shi,{ddagger} Gongxun Zhong,{ddagger} Guohua Deng,{ddagger} Guobin Tian, Jinying Ge, Xianying Zeng, Jiasheng Song, Dongming Zhao, Liling Liu, Yongping Jiang, Yuntao Guan, Zhigao Bu, and Hualan Chen*
Animal Influenza Laboratory of the Ministry of Agriculture and National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin 150001, People's Republic of China
Received 24 February 2010/ Accepted 28 May 2010
Despite substantial efforts to control H5N1 avian influenza viruses (AIVs), the viruses have continued to evolve and cause disease outbreaks in poultry and infections in humans. In this report, we analyzed 51 representative H5N1 AIVs isolated from domestic poultry, wild birds, and humans in China during 2004 to 2009, and 21 genotypes were detected based on whole-genome sequences. Twelve genotypes of AIVs in southern China bear similar H5 hemagglutinin (HA) genes (clade 2.3). These AIVs did not display antigenic drift and could be completely protected against by the A/goose/Guangdong/1/96 (GS/GD/1/96)-based oil-adjuvanted killed vaccine and recombinant Newcastle disease virus vaccine, which have been used in China. In addition, antigenically drifted H5N1 viruses, represented by A/chicken/Shanxi/2/06 (CK/SX/2/06), were detected in chickens from several provinces in northern China. The CK/SX/2/06-like viruses are reassortants with newly emerged HA, NA, and PB1 genes that could not be protected against by the GS/GD/1/96-based vaccines. These viruses also reacted poorly with antisera generated from clade 2.2 and 2.3 viruses. The majority of the viruses isolated from southern China were lethal in mice and ducks, while the CK/SX/2/06-like viruses caused mild disease in mice and could not replicate in ducks. Our results demonstrate that the H5N1 AIVs circulating in nature have complex biological characteristics and pose a continued challenge for disease control and pandemic preparedness.
* Corresponding author. Mailing address: Harbin Veterinary Research Institute, 427 Maduan Street, Harbin 150001, People's Republic of China. Phone: 86-451-82761925. Fax: 86-451-82733132. E-mail: hlchen1@yahoo.com
{triangledown} Published ahead of print on 10 June 2010.
{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.
{ddagger} Y.L., J.S., G.Z., and G.D. equally contributed to this paper.
Journal of Virology, September 2010, p. 8389-8397, Vol. 84, No. 17
0022-538X/10/$012.00+0 doi:10.1128/JVI.00413-10
Copyright ? 2010, American Society for Microbiology. All Rights Reserved.
