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J Virol Methods. 2018 Jun 22. pii: S0166-0934(18)30152-6. doi: 10.1016/j.jviromet.2018.06.011. [Epub ahead of print]
Viral interference between Low Pathogenic Avian Influenza H9N2 and avian infectious bronchitis viruses in vitro and in ovo.
Rim A1, Nacira L2, Abdeljelil G3.
Author information
Abstract
BACKGROUND:
Low pathogenic avian influenza (LPAI) H9N2 and infectious bronchitis virus (IBV) are important pathogens of poultry, causing important economic losses for the sector. Replication interference between these two viruses was described using cell cultures (CC) and embryonated chicken eggs (ECE). Chicken embryo lung (CEL) and ECE were simultaneously or sequentially infected with IBV vaccine strain (H120) and LPAIV-H9N2 (A/Ck/TUN/145/2012) to evaluate viral interactionsin vitro and in ovo, respectively. Real-time RT-PCR was developed to specifically quantify both AIV and IBV genomes as well as viral gene copy numbers during mixed infections. The amount of IL-1 beta, in supernatants of co-infected cell cultures, was determined using an ELISA assay.
RESULTS:
Quantitative results of AIV and IBV co-infection showed that interferences between the two viruses yielded decreased viral growth. However, in the case of super-infection, the second virus, either AIV or IBV, induced a decrease in the growth of the first inoculated virus.
CONCLUSION:
It appears that either AIV or IBV has a negative impact on the other virus growth when they are inoculated simultaneously or sequentially. The ELISA results showed that higher level of secreted IL-1beta varies, depending on the viral interference conditions between both viruses, during mixed infections.
Copyright ? 2018. Published by Elsevier B.V.
KEYWORDS:
Viral interference; avian infectious bronchitis disease virus; avian influenza virus; cytokine; real-time RT-PCR
PMID: 29940196 DOI: 10.1016/j.jviromet.2018.06.011
Viral interference between Low Pathogenic Avian Influenza H9N2 and avian infectious bronchitis viruses in vitro and in ovo.
Rim A1, Nacira L2, Abdeljelil G3.
Author information
Abstract
BACKGROUND:
Low pathogenic avian influenza (LPAI) H9N2 and infectious bronchitis virus (IBV) are important pathogens of poultry, causing important economic losses for the sector. Replication interference between these two viruses was described using cell cultures (CC) and embryonated chicken eggs (ECE). Chicken embryo lung (CEL) and ECE were simultaneously or sequentially infected with IBV vaccine strain (H120) and LPAIV-H9N2 (A/Ck/TUN/145/2012) to evaluate viral interactionsin vitro and in ovo, respectively. Real-time RT-PCR was developed to specifically quantify both AIV and IBV genomes as well as viral gene copy numbers during mixed infections. The amount of IL-1 beta, in supernatants of co-infected cell cultures, was determined using an ELISA assay.
RESULTS:
Quantitative results of AIV and IBV co-infection showed that interferences between the two viruses yielded decreased viral growth. However, in the case of super-infection, the second virus, either AIV or IBV, induced a decrease in the growth of the first inoculated virus.
CONCLUSION:
It appears that either AIV or IBV has a negative impact on the other virus growth when they are inoculated simultaneously or sequentially. The ELISA results showed that higher level of secreted IL-1beta varies, depending on the viral interference conditions between both viruses, during mixed infections.
Copyright ? 2018. Published by Elsevier B.V.
KEYWORDS:
Viral interference; avian infectious bronchitis disease virus; avian influenza virus; cytokine; real-time RT-PCR
PMID: 29940196 DOI: 10.1016/j.jviromet.2018.06.011