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Synthetically derived bat influenza A-like viruses reveal a cell type- but not species-specific tropism
Significance
Since the discovery of bat influenza A-like genomic sequences (provisionally designated HL17NL10 and HL18NL11), it was uncertain whether these sequences encode infectious viruses and, if so, which cells might support propagation of these viruses. Using chimeric vesicular stomatitis virus (VSV) encoding HL18 or HL17, a particular cell line of canine origin was found to be highly susceptible to infection. Taking advantage of this cell line, we succeeded to generate recombinant HL17NL10 and HL18NL11. These viruses revealed marked differences to conventional influenza A viruses because they do not use the same receptor, in addition to initiating infection from the basolateral site of polarized epithelial cells. The established reverse genetic system will undoubtedly help characterizing these hitherto uncultivable viruses.
Abstract
Two novel influenza A-like viral genome sequences have recently been identified in Central and South American fruit bats and provisionally designated ?HL17NL10? and ?HL18NL11.? All efforts to isolate infectious virus from bats or to generate these viruses by reverse genetics have failed to date. Recombinant vesicular stomatitis virus (VSV) encoding the hemagglutinin-like envelope glycoproteins HL17 or HL18 in place of the VSV glycoprotein were generated to identify cell lines that are susceptible to bat influenza A-like virus entry. More than 30 cell lines derived from various species were screened but only a few cell lines were found to be susceptible, including Madin?Darby canine kidney type II (MDCK II) cells. The identification of cell lines susceptible to VSV chimeras allowed us to recover recombinant HL17NL10 and HL18NL11 viruses from synthetic DNA. Both influenza A-like viruses established a productive infection in MDCK II cells; however, HL18NL11 replicated more efficiently than HL17NL10 in this cell line. Unlike conventional influenza A viruses, bat influenza A-like viruses started the infection preferentially at the basolateral membrane of polarized MDCK II cells; however, similar to conventional influenza A viruses, bat influenza A-like viruses were released primarily from the apical site. The ability of HL18NL11 or HL17NL10 viruses to infect canine and human cells might reflect a zoonotic potential of these recently identified bat viruses.
- ?tori Aguiar Moreiraa,b,c,
- Samira Locherd,
- Larissa Kolesnikovae,
- Hardin Boltea,b,c,
- Teresa Aydillof,g,
- Adolfo Garc?a-Sastref,g,h,
- Martin Schwemmlea,1, and
- Gert Zimmerd,1
- aInstitute of Virology, University Medical Center Freiburg, D-79104 Freiburg, Germany;
- bSpemann Graduate School of Biology and Medicine, University of Freiburg, D-79104 Freiburg, Germany;
- cFaculty of Biology, University of Freiburg, D-79104 Freiburg, Germany;
- dDivision of Virology, Institute of Virology and Immunology, CH-3147 Mittelh?usern, Switzerland;
- eInstitute of Virology, Philipps-University, D-35043 Marburg, Germany;
- fDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029;
- gGlobal Health and Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029;
- hDepartment of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, NY 10029
- Edited by Robert A. Lamb, Howard Hughes Medical Institute and Northwestern University, Evanston, IL, and approved September 19, 2016 (received for review June 1, 2016)
Significance
Since the discovery of bat influenza A-like genomic sequences (provisionally designated HL17NL10 and HL18NL11), it was uncertain whether these sequences encode infectious viruses and, if so, which cells might support propagation of these viruses. Using chimeric vesicular stomatitis virus (VSV) encoding HL18 or HL17, a particular cell line of canine origin was found to be highly susceptible to infection. Taking advantage of this cell line, we succeeded to generate recombinant HL17NL10 and HL18NL11. These viruses revealed marked differences to conventional influenza A viruses because they do not use the same receptor, in addition to initiating infection from the basolateral site of polarized epithelial cells. The established reverse genetic system will undoubtedly help characterizing these hitherto uncultivable viruses.
Abstract
Two novel influenza A-like viral genome sequences have recently been identified in Central and South American fruit bats and provisionally designated ?HL17NL10? and ?HL18NL11.? All efforts to isolate infectious virus from bats or to generate these viruses by reverse genetics have failed to date. Recombinant vesicular stomatitis virus (VSV) encoding the hemagglutinin-like envelope glycoproteins HL17 or HL18 in place of the VSV glycoprotein were generated to identify cell lines that are susceptible to bat influenza A-like virus entry. More than 30 cell lines derived from various species were screened but only a few cell lines were found to be susceptible, including Madin?Darby canine kidney type II (MDCK II) cells. The identification of cell lines susceptible to VSV chimeras allowed us to recover recombinant HL17NL10 and HL18NL11 viruses from synthetic DNA. Both influenza A-like viruses established a productive infection in MDCK II cells; however, HL18NL11 replicated more efficiently than HL17NL10 in this cell line. Unlike conventional influenza A viruses, bat influenza A-like viruses started the infection preferentially at the basolateral membrane of polarized MDCK II cells; however, similar to conventional influenza A viruses, bat influenza A-like viruses were released primarily from the apical site. The ability of HL18NL11 or HL17NL10 viruses to infect canine and human cells might reflect a zoonotic potential of these recently identified bat viruses.
