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Vet Microbiol. 2013 Jun 14. pii: S0378-1135(13)00295-2. doi: 10.1016/j.vetmic.2013.05.013. [Epub ahead of print]
Characterization of recombinant H9N2 influenza viruses isolated from wild ducks in China.
Zhu G, Wang R, Xuan F, Daszak P, Anthony SJ, Zhang S, Zhang L, He G.
Source
Institutes for Advanced Studies in Multidisciplinary Science and Technology, East China Normal University, Shanghai, People's Republic of China; Guangdong Entomological Institute, Guangzhou, People's Republic of China; EcoHealth Alliance, 460 West 34th Street, New York, USA.
Abstract
Wild birds are considered to be the natural reservoirs for avian influenza A viruses (AIV). During active influenza surveillance in Poyang Lake of southeast China, we isolated and characterized 11 H9N2 viruses from two species of wild ducks. Phylogenetic analysis showed that the 11 isolates were almost identical with 99.3-100% nucleotide homology in their entire genome, and they all closely related in whole eight genes (95.6-99.4% homology) to human H9N2 isolates (HK/33982/2009) and clustered in the same sublineage. The isolates belonged to triple reassortant H9N2 genotype viruses containing Ck/Bei-like NA genes, Y439-like PA genes and six other G1-like genes. We also found that the subtype of virus replicated efficiently in the lungs and tracheas of BALB/c mice and caused mortality in 20-40% of infected groups after 3-6 days, which indicates that the subtype of virus is capable of establishing lethal mammalian infections. However, whether or not the virus has features transmittable from wild ducks to humans is not known. This study showed that the subtype of virus was detected for the first time in wild birds, and also suggested that wild birds may carry the virus for a long time and spread it over long distances along migratory routes, so more attention should be paid to the continued surveillance of wild birds.
Copyright ? 2013 Elsevier B.V. All rights reserved.
KEYWORDS:
H9N2, Human infection, Triple reassortant, Wild ducks
PMID:
23830774
[PubMed - as supplied by publisher]
Characterization of recombinant H9N2 influenza viruses isolated from wild ducks in China.
Zhu G, Wang R, Xuan F, Daszak P, Anthony SJ, Zhang S, Zhang L, He G.
Source
Institutes for Advanced Studies in Multidisciplinary Science and Technology, East China Normal University, Shanghai, People's Republic of China; Guangdong Entomological Institute, Guangzhou, People's Republic of China; EcoHealth Alliance, 460 West 34th Street, New York, USA.
Abstract
Wild birds are considered to be the natural reservoirs for avian influenza A viruses (AIV). During active influenza surveillance in Poyang Lake of southeast China, we isolated and characterized 11 H9N2 viruses from two species of wild ducks. Phylogenetic analysis showed that the 11 isolates were almost identical with 99.3-100% nucleotide homology in their entire genome, and they all closely related in whole eight genes (95.6-99.4% homology) to human H9N2 isolates (HK/33982/2009) and clustered in the same sublineage. The isolates belonged to triple reassortant H9N2 genotype viruses containing Ck/Bei-like NA genes, Y439-like PA genes and six other G1-like genes. We also found that the subtype of virus replicated efficiently in the lungs and tracheas of BALB/c mice and caused mortality in 20-40% of infected groups after 3-6 days, which indicates that the subtype of virus is capable of establishing lethal mammalian infections. However, whether or not the virus has features transmittable from wild ducks to humans is not known. This study showed that the subtype of virus was detected for the first time in wild birds, and also suggested that wild birds may carry the virus for a long time and spread it over long distances along migratory routes, so more attention should be paid to the continued surveillance of wild birds.
Copyright ? 2013 Elsevier B.V. All rights reserved.
KEYWORDS:
H9N2, Human infection, Triple reassortant, Wild ducks
PMID:
23830774
[PubMed - as supplied by publisher]
