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Nat Biomed Eng
. 2023 Sep 21.
doi: 10.1038/s41551-023-01092-4. Online ahead of print. Multiplexed discrimination of SARS-CoV-2 variants via plasmonic-enhanced fluorescence in a portable and automated device
Ying Liu # 1 , Yang Yang # 2 , Guanghui Wang # 1 , Dou Wang # 1 , Pan-Lin Shao 3 , Jiahu Tang 1 , Tingzhen He 1 , Jintao Zheng 1 , Ruibin Hu 1 , Yiyi Liu 1 , Ziyi Xu 1 , Dan Niu 1 , Jiahui Lv 1 , Jingkai Yang 1 , Hongjun Xiao 1 , Shuai Wu 2 , Shuang He 1 , Zhongrong Tang 1 , Yan Liu 2 , Meijie Tang 4 , Xingyu Jiang 5 , Jing Yuan 6 , Hongjie Dai 7 , Bo Zhang 8
Affiliations
Portable assays for the rapid identification of lineages of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are needed to aid large-scale efforts in monitoring the evolution of the virus. Here we report a multiplexed assay in a microarray format for the detection, via isothermal amplification and plasmonic-gold-enhanced near-infrared fluorescence, of variants of SARS-CoV-2. The assay, which has single-nucleotide specificity for variant discrimination, single-RNA-copy sensitivity and does not require RNA extraction, discriminated 12 lineages of SARS-CoV-2 (in three mutational hotspots of the Spike protein) and detected the virus in nasopharyngeal swabs from 1,034 individuals at 98.8% sensitivity and 100% specificity, with 97.6% concordance with genome sequencing in variant discrimination. We also report a compact, portable and fully automated device integrating the entire swab-to-result workflow and amenable to the point-of-care detection of SARS-CoV-2 variants. Portable, rapid, accurate and multiplexed assays for the detection of SARS-CoV-2 variants and lineages may facilitate variant-surveillance efforts.
. 2023 Sep 21.
doi: 10.1038/s41551-023-01092-4. Online ahead of print. Multiplexed discrimination of SARS-CoV-2 variants via plasmonic-enhanced fluorescence in a portable and automated device
Ying Liu # 1 , Yang Yang # 2 , Guanghui Wang # 1 , Dou Wang # 1 , Pan-Lin Shao 3 , Jiahu Tang 1 , Tingzhen He 1 , Jintao Zheng 1 , Ruibin Hu 1 , Yiyi Liu 1 , Ziyi Xu 1 , Dan Niu 1 , Jiahui Lv 1 , Jingkai Yang 1 , Hongjun Xiao 1 , Shuai Wu 2 , Shuang He 1 , Zhongrong Tang 1 , Yan Liu 2 , Meijie Tang 4 , Xingyu Jiang 5 , Jing Yuan 6 , Hongjie Dai 7 , Bo Zhang 8
Affiliations
- PMID: 37735541
- DOI: 10.1038/s41551-023-01092-4
Portable assays for the rapid identification of lineages of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are needed to aid large-scale efforts in monitoring the evolution of the virus. Here we report a multiplexed assay in a microarray format for the detection, via isothermal amplification and plasmonic-gold-enhanced near-infrared fluorescence, of variants of SARS-CoV-2. The assay, which has single-nucleotide specificity for variant discrimination, single-RNA-copy sensitivity and does not require RNA extraction, discriminated 12 lineages of SARS-CoV-2 (in three mutational hotspots of the Spike protein) and detected the virus in nasopharyngeal swabs from 1,034 individuals at 98.8% sensitivity and 100% specificity, with 97.6% concordance with genome sequencing in variant discrimination. We also report a compact, portable and fully automated device integrating the entire swab-to-result workflow and amenable to the point-of-care detection of SARS-CoV-2 variants. Portable, rapid, accurate and multiplexed assays for the detection of SARS-CoV-2 variants and lineages may facilitate variant-surveillance efforts.