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Exp Ther Med
. 2023 Jul 6;26(2):398.
doi: 10.3892/etm.2023.12097. eCollection 2023 Aug. Reverse transcription loop‑mediated isothermal amplification has a high performance in the detection of SARS‑CoV‑2 in saliva samples and nasal swabs from asymptomatic and symptomatic individuals
Rodolfo Reyes-Morales 1 , Patricia Segundo-Ibañez 2 , César Flores-de Los Ángeles 3 , David Vizcarra-Ramos 2 , Damián Iñaki Ibañez-Galeana 2 , Gabriela Salas-Cuevas 4 , Ángel Olvera-Serrano 4 , Nancy Bibiana Pérez-Silva 3 , Valeria Magali Rocha-Rocha 5 , Elie Girgis El-Kassi 5 , Jorge Escobedo-Straffon 5 , Laura Contreras-Mioni 5 , Marisol Rosas-Díaz 6 , Karla María Lopez-Martinez 1 , Carlos Eduardo Arias-Matus 1 , Elizabeth Bautista-Rodriguez 1 , Manuel Nolasco-Quiroga 4
Affiliations
The detection of coronavirus disease 2019 cases represents a significant challenge at the epidemiological level. Limitations exist in effectively detecting asymptomatic cases, achieving good follow-up in hospitals without the infrastructure for reverse transcription-quantitative PCR (RT-qPCR) or in difficult-to-access areas and developing methods with the need for less invasive sampling procedures. Therefore, the present study evaluated the performance of the direct reverse transcription loop-mediated isothermal amplification (RT-LAMP) test for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the saliva and nasal samples of asymptomatic individuals belonging to the university population. In addition, this test was also assessed for effectiveness in symptomatic individuals referred from a hospital with poor infrastructure in molecular biology and located outside the urban area. The RT-LAMP assay was compared with the results obtained from the RT-qPCR nasopharyngeal swab test, where the diagnosis was confirmed by lateral flow immunoassay test for rapid antigen detection. A total of 128 samples were analyzed, of which 43% were symptomatic positive individuals, 25% were asymptomatic positive individuals and 32% were SARS-CoV2-negative control individuals. Among positive individuals, no differences were found between the Cq values determined by RT-qPCR. A sensitivity of 96.5% and a specificity of 97.6% was reported for the detection of SARS-CoV-2 in symptomatic individuals by salivary and nasal RT-LAMP, as well as a sensitivity of 100% and a specificity of 97.6% for the detection of SARS-CoV-2 in asymptomatic individuals. These findings indicated that performance of the direct RT-LAMP test using saliva and nasal samples has high sensitivity and specificity, which in turn suggest that it is a viable and reliable alternative for use in epidemiological monitoring.
Keywords: asymptomatic; nasal swab; reverse transcription loop-mediated isothermal amplification; saliva; severe acute respiratory syndrome coronavirus 2; symptomatic.
. 2023 Jul 6;26(2):398.
doi: 10.3892/etm.2023.12097. eCollection 2023 Aug. Reverse transcription loop‑mediated isothermal amplification has a high performance in the detection of SARS‑CoV‑2 in saliva samples and nasal swabs from asymptomatic and symptomatic individuals
Rodolfo Reyes-Morales 1 , Patricia Segundo-Ibañez 2 , César Flores-de Los Ángeles 3 , David Vizcarra-Ramos 2 , Damián Iñaki Ibañez-Galeana 2 , Gabriela Salas-Cuevas 4 , Ángel Olvera-Serrano 4 , Nancy Bibiana Pérez-Silva 3 , Valeria Magali Rocha-Rocha 5 , Elie Girgis El-Kassi 5 , Jorge Escobedo-Straffon 5 , Laura Contreras-Mioni 5 , Marisol Rosas-Díaz 6 , Karla María Lopez-Martinez 1 , Carlos Eduardo Arias-Matus 1 , Elizabeth Bautista-Rodriguez 1 , Manuel Nolasco-Quiroga 4
Affiliations
- PMID: 37522063
- PMCID: PMC10375439
- DOI: 10.3892/etm.2023.12097
The detection of coronavirus disease 2019 cases represents a significant challenge at the epidemiological level. Limitations exist in effectively detecting asymptomatic cases, achieving good follow-up in hospitals without the infrastructure for reverse transcription-quantitative PCR (RT-qPCR) or in difficult-to-access areas and developing methods with the need for less invasive sampling procedures. Therefore, the present study evaluated the performance of the direct reverse transcription loop-mediated isothermal amplification (RT-LAMP) test for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the saliva and nasal samples of asymptomatic individuals belonging to the university population. In addition, this test was also assessed for effectiveness in symptomatic individuals referred from a hospital with poor infrastructure in molecular biology and located outside the urban area. The RT-LAMP assay was compared with the results obtained from the RT-qPCR nasopharyngeal swab test, where the diagnosis was confirmed by lateral flow immunoassay test for rapid antigen detection. A total of 128 samples were analyzed, of which 43% were symptomatic positive individuals, 25% were asymptomatic positive individuals and 32% were SARS-CoV2-negative control individuals. Among positive individuals, no differences were found between the Cq values determined by RT-qPCR. A sensitivity of 96.5% and a specificity of 97.6% was reported for the detection of SARS-CoV-2 in symptomatic individuals by salivary and nasal RT-LAMP, as well as a sensitivity of 100% and a specificity of 97.6% for the detection of SARS-CoV-2 in asymptomatic individuals. These findings indicated that performance of the direct RT-LAMP test using saliva and nasal samples has high sensitivity and specificity, which in turn suggest that it is a viable and reliable alternative for use in epidemiological monitoring.
Keywords: asymptomatic; nasal swab; reverse transcription loop-mediated isothermal amplification; saliva; severe acute respiratory syndrome coronavirus 2; symptomatic.