Nat Biomed Eng . A lab-on-a-chip for the concurrent electrochemical detection of SARS-CoV-2 RNA and anti-SARS-CoV-2 antibodies in saliva and plasma

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Nat Biomed Eng


. 2022 Aug 8.
doi: 10.1038/s41551-022-00919-w. Online ahead of print.
A lab-on-a-chip for the concurrent electrochemical detection of SARS-CoV-2 RNA and anti-SARS-CoV-2 antibodies in saliva and plasma


Devora Najjar ^{# 1 2 3} , Joshua Rainbow ^{# 1 4} , Sanjay Sharma Timilsina ^{# 1} , Pawan Jolly ^{# 1} , Helena de Puig ^{# 1 2} , Mohamed Yafia ¹ , Nolan Durr ¹ , Hani Sallum ¹ , Galit Alter ⁵ , Jonathan Z Li ⁶ , Xu G Yu ^{5 6} , David R Walt ^{1 7 8} , Joseph A Paradiso ³ , Pedro Estrela ⁴ , James J Collins ^{9 10 11} , Donald E Ingber ^{12 13 14 15}



Affiliations

• PMID: 35941191
• DOI: 10.1038/s41551-022-00919-w

Abstract

Rapid, accurate and frequent detection of the RNA of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) and of serological host antibodies to the virus would facilitate the determination of the immune status of individuals who have Coronavirus disease 2019 (COVID-19), were previously infected by the virus, or were vaccinated against the disease. Here we describe the development and application of a 3D-printed lab-on-a-chip that concurrently detects, via multiplexed electrochemical outputs and within 2 h, SARS-CoV-2 RNA in saliva as well as anti-SARS-CoV-2 immunoglobulins in saliva spiked with blood plasma. The device automatedly extracts, concentrates and amplifies SARS-CoV-2 RNA from unprocessed saliva, and integrates the Cas12a-based enzymatic detection of SARS-CoV-2 RNA via isothermal nucleic acid amplification with a sandwich-based enzyme-linked immunosorbent assay on electrodes functionalized with the Spike S1, nucleocapsid and receptor-binding-domain antigens of SARS-CoV-2. Inexpensive microfluidic electrochemical sensors for performing multiplexed diagnostics at the point of care may facilitate the widespread monitoring of COVID-19 infection and immunity.