Sens Actuators B Chem . Paper-based netlike rolling circle amplification (NRCA) for ultrasensitive and visual detection of SARS-CoV-2

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Sens Actuators B Chem


. 2022 Jan 22;131460.
doi: 10.1016/j.snb.2022.131460. Online ahead of print.
Paper-based netlike rolling circle amplification (NRCA) for ultrasensitive and visual detection of SARS-CoV-2


Yuchen Song ¹ , Yuqing Chao ² , Yi Guo ¹ , Fan Zhang ¹ , Changqing Mao ¹ , Chaoyang Guan ¹ , Guifang Chen ¹ , Chang Feng ¹



Affiliations

• PMID: 35095201
• PMCID: PMC8782770
• DOI: 10.1016/j.snb.2022.131460

Abstract

COVID-19 is a highly diffuse respiratory infection caused by severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2). Currently, quantitative real-time polymerase chain reaction (qRT-PCR) technology is commonly used in clinical diagnosis of COVID-19. However, this method is time-consuming and labor-intensive, which is limited in clinical application. Here, we propose a new method for the ultrasensitive and visual detection of SARS-CoV-2 viral nucleic acid. The assay integrates with a paper device and highly efficient isothermal amplification technology - Netlike rolling circle amplification (NRCA), which can reach a limit of detection of 4.12 aM. The paper-based NRCA owns advantages of specificity, portability, visualization and low-cost. Therefore, this method can effectively meet the requirements of point-of-care testing, providing a novel molecular detection technology for clinical diagnosis of COVID-19 and promoting the development of NRCA devices.

Keywords: Netlike rolling circle amplification; Paper; Point-of-care testing; SARS-CoV-2.