Talanta . Electrochemical immunosensor for rapid and highly sensitive detection of SARS-CoV-2 antigen in the nasal sample

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Talanta


. 2022 Jan 5;240:123211.
doi: 10.1016/j.talanta.2022.123211. Online ahead of print.
Electrochemical immunosensor for rapid and highly sensitive detection of SARS-CoV-2 antigen in the nasal sample


Mohammad Mehmandoust ¹ , Z Pinar Gumus ² , Mustafa Soylak ³ , Nevin Erk ⁴



Affiliations

• PMID: 34999319
• DOI: 10.1016/j.talanta.2022.123211

Abstract

A label-free electrochemical biosensing approach as an appropriate analysis technique for SARS-CoV-2 spike protein (SARS-CoV-2 S-protein) was investigated to facilitate the diagnosis of coronavirus in real samples. It is crucial to construct diagnostic features that can rapidly identify infected individuals to limit the spread of the virus and assign treatment choices. Therefore, a novel and selective method using SiO₂@UiO-66 and a label-free electrochemical immunoassay for rapidly detecting spike protein. The development of innovative approaches for direct viral detection employing simplified and ideally reagent-free assays is a pressing and difficult topic. The absence of speedy and effective ways to diagnose viral diseases especially SARS-CoV-2 on demand has worsened the issue of combating the COVID-19 pandemic. The developed electrode illustrated a wide dynamic range of 100.0 fg mL^-1 to 10.0 ng mL^-1 with low limit detection. Therefore, the as-fabricated electrochemical SARS-CoV-2 S-protein sensor suggests an appropriate perspective in the point-of-care system, within 5.0 min, in nasal samples with satisfactory recovery.

Keywords: COVID-19; Metal-organic frameworks composite; Nasal sample; Spike protein.