Expert Rev Mol Diagn . Potential of CRISPR/Cas system in the diagnosis of COVID-19 infection

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Expert Rev Mol Diagn


. 2021 Aug 19.
doi: 10.1080/14737159.2021.1970535. Online ahead of print.
Potential of CRISPR/Cas system in the diagnosis of COVID-19 infection


V Edwin Hillary ¹ , Savarimuthu Ignacimuthu ² , Stanislaus Antony Ceasar ^{3 4}



Affiliations

• PMID: 34409907
• DOI: 10.1080/14737159.2021.1970535

Abstract

Introduction: Emerging novel infectious diseases and persistent pandemics with potential to destabilize normal life remain a public health concern for the whole world. The recent outbreak of pneumonia caused by Coronavirus infectious disease-2019 (COVID-19) resulted in high mortality due to a lack of effective drugs or vaccines. With a constantly increasing number of infections with mutated strains and deaths across the globe, rapid, affordable and specific detections with more accurate diagnosis and improved health treatments are needed to combat the spread of this novel pathogen COVID-19.
Areas covered: Researchers have started to utilize the recently invented clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR associated proteins (CRISPR/Cas)-based tools for the rapid detection of novel COVID-19. In this review, we summarize the potential of CRISPR/Cas system for the diagnosis and enablement of efficient control of COVID-19.
Expert opinion: Multiple groups have demonstrated the potential of utilizing CRISPR-based diagnosis tools for the detection of SARS-CoV-2. In coming months, we expect more novel and rapid CRISPR-based kits for mass detection of COVID-19 infected persons within a fraction of a second. Therefore, we believe science will conquer COVID-19 in the near future and CRISPR/Cas system may play a key role in the efficient diagnosis and treatment of this mass-spreading disease.

Keywords: COVID-19; CRISPR/Cas; CRISPR/Cas12; CRISPR/Cas13; CRISPR/Cas9; DETECTR; SHERLOCK; diagnostics; infectious disease.