Jpn J Infect Dis . An Ultra-Rapid Real-Time RT-PCR Method Using the PCR1100 to Detect Severe Acute Respiratory Syndrome Coronavirus-2

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Jpn J Infect Dis


. 2020 Jun 30.
doi: 10.7883/yoken.JJID.2020.324. Online ahead of print.
An Ultra-Rapid Real-Time RT-PCR Method Using the PCR1100 to Detect Severe Acute Respiratory Syndrome Coronavirus-2

Kazuya Shirato ¹ , Naganori Nao ¹ , Shutoku Matsuyama ¹ , Makoto Takeda ¹ , Tsutomu Kageyama ²


AffiliationsExpand

• PMID: 32611983
• DOI: 10.7883/yoken.JJID.2020.324

Abstract

The disease caused by severe acute respiratory syndrome coronavirus (SARS-CoV-2) in Wuhan, China, in December 2019 is currently spreading rapidly worldwide. SARS-CoV-2 is usually detected via real-time RT-PCR. However, as institutions/hospitals deal with increasing numbers of specimens, a simpler detection system is required. Here, we present an ultra-rapid, real-time RT-PCR assay for SARS-CoV-2 using the PCR1100 device. Although this tests only one specimen at any one time, the amplification period is <20 min, with maintenance of the sensitivity and specificity of conventional real-time RT-PCR performed using large instruments. The method will be very helpful if SARS-CoV-2 testing is required a few times daily, for example to confirm virus-free status prior to discharge.

Keywords: PCR1100; Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); ultra-rapid real-time RT-PCR.