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Viral Immunol. 2018 May 21. doi: 10.1089/vim.2017.0201. [Epub ahead of print]
Microparticle Release from Cell Lines and Its Anti-influenza Activity.
Jantaratrirat S1, Boonarkart C1, Ruangrung K1, Suptawiwat O2, Auewarakul P1.
Author information
Abstract
Microparticles (MPs) are vesicles that are released by budding from plasma membrane of living cells. Recently, the role of MPs in antiviral activity has been proposed. We investigated quantity and anti-influenza activity of MPs from human alveolar epithelial cells A549, human bronchial epithelial cells BEAS-2B, human colon adenocarcinoma cells HT-29, and the human lung fibroblast cells MRC-5. MPs were found from all four cell lines. However, anti-influenza activity against an H1N1 influenza virus was found only from MPs of A549 and BEAS-2B. BEAS-2B cell differentiation did not increase MP release. Methyl-β-cyclodextrin (MβCD) increased MP release and anti-influenza activity in HT-29 and A549. MP release increased after calcium ionophore A23187 treatment in three cell lines but only in HT-29 after forskolin treatment. These findings provide in vitro data supporting the role of MPs as an innate defense against influenza virus and as an approach to enhance the defense.
KEYWORDS:
antiviral activity; influenza; methyl-β-cyclodextrin; microparticle
PMID: 29782226 DOI: 10.1089/vim.2017.0201
Microparticle Release from Cell Lines and Its Anti-influenza Activity.
Jantaratrirat S1, Boonarkart C1, Ruangrung K1, Suptawiwat O2, Auewarakul P1.
Author information
Abstract
Microparticles (MPs) are vesicles that are released by budding from plasma membrane of living cells. Recently, the role of MPs in antiviral activity has been proposed. We investigated quantity and anti-influenza activity of MPs from human alveolar epithelial cells A549, human bronchial epithelial cells BEAS-2B, human colon adenocarcinoma cells HT-29, and the human lung fibroblast cells MRC-5. MPs were found from all four cell lines. However, anti-influenza activity against an H1N1 influenza virus was found only from MPs of A549 and BEAS-2B. BEAS-2B cell differentiation did not increase MP release. Methyl-β-cyclodextrin (MβCD) increased MP release and anti-influenza activity in HT-29 and A549. MP release increased after calcium ionophore A23187 treatment in three cell lines but only in HT-29 after forskolin treatment. These findings provide in vitro data supporting the role of MPs as an innate defense against influenza virus and as an approach to enhance the defense.
KEYWORDS:
antiviral activity; influenza; methyl-β-cyclodextrin; microparticle
PMID: 29782226 DOI: 10.1089/vim.2017.0201