Front Immunol . B cell receptor repertoire analysis from autopsy samples of COVID-19 patients

[tetano]

Front Immunol


. 2023 Feb 23;14:1034978.
doi: 10.3389/fimmu.2023.1034978. eCollection 2023.
B cell receptor repertoire analysis from autopsy samples of COVID-19 patients


Sadahiro Iwabuchi ¹ , Tomohide Tsukahara ² , Toshitugu Okayama ³ , Masahiro Kitabatake ⁴ , Hideki Motobayashi ⁵ , Shigeyuki Shichino ⁶ , Tadashi Imafuku ¹ , Kenzaburo Yamaji ⁷ , Kyohei Miyamoto ⁸ , Shinobu Tamura ⁸ , Satoshi Ueha ⁶ , Toshihiro Ito ⁴ , Shin-Ichi Murata ⁹ , Toshikazu Kondo ¹⁰ , Kazuho Ikeo ³ , Yutaka Suzuki ¹¹ , Kouji Matsushima ⁶ , Michinori Kohara ⁷ , Toshihiko Torigoe ² , Hiroki Yamaue ^{5 12} , Shinichi Hashimoto ¹



Affiliations

• PMID: 36911681
• PMCID: PMC9996338
• DOI: 10.3389/fimmu.2023.1034978

Abstract

Neutralizing antibodies against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are being developed world over. We investigated the possibility of producing artificial antibodies from the formalin fixation and paraffin-embedding (FFPE) lung lobes of a patient who died by coronavirus disease 2019 (COVID-19). The B-cell receptors repertoire in the lung tissue where SARS-CoV-2 was detected were considered to have highly sensitive virus-neutralizing activity, and artificial antibodies were produced by combining the most frequently detected heavy and light chains. Some neutralizing effects against the SARS-CoV-2 were observed, and mixing two different artificial antibodies had a higher tendency to suppress the virus. The neutralizing effects were similar to the immunoglobulin G obtained from healthy donors who had received a COVID-19 mRNA vaccine. Therefore, the use of FFPE lung tissue, which preserves the condition of direct virus sensitization, to generate artificial antibodies may be useful against future unknown infectious diseases.

Keywords: B cell receptors; COVID-19; lung; serum IgGs; single cell; vaccine intake.