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Front Immunol
. 2025 Nov 19:16:1628102.
doi: 10.3389/fimmu.2025.1628102. eCollection 2025. Transplacental transferred anti-SARS-CoV-2 neutralizing antibodies in unvaccinated pregnant women in Cameroon occurred during the COVID-19 pandemic, but not in the pre-pandemic period
Reine Medouen Ndeumou Seumko'o 1 2 3 , Thandeka Moyo-Gwete 4 5 , Tandile Hermanus 4 5 , Sosthene Hillary Matabou Tene 2 3 6 , Romeo Brice Dieffouo Djounda 2 3 6 , Chris Marco Mbianda Nana 1 2 , Bernard Marie Zambo Bitye 1 2 , Tekougang Berenice Kenfack Zangue 1 2 7 , Bodin Darcisse Kwanou Tchakounte 1 2 , Eitel Mpoudi Ngolle 3 , Diane Wallace Taylor 8 , Rose Gana Fomban Leke 2 3 , Rosette Megnekou 1 2 , Penny L Moore 4 5 9 10 , Livo Forgu Esemu 2 3 11
Affiliations
Introduction: Neutralizing antibodies (NAbs) are critical for protection against SARS-CoV-2, but there is limited information on their role in pregnancy, especially among Cameroonian women. Here, we aimed to determine the prevalence of pan-coronavirus reactive antibodies from pregnant women sampled before and during the COVID-19 pandemic.
Methods: Plasma samples from 629 women in the second trimester and 661 at delivery were collected pre-COVID-19 and from 39 women at delivery during COVID-19 in Yaoundé, Cameroon. All samples were screened using the Abbott Panbio™ COVID-19 rapid diagnostic test (RDT). Enzyme-linked immunosorbent assays (ELISA) and the spike-pseudotyped lentivirus neutralization assay were done to measure antibody binding and neutralizing capacity in 118 and 33 samples, respectively.
Results: Before the pandemic, 16.5% (213/1290) of pregnant women were seropositive for cross-reactive anti-SARS-CoV-2 antibodies by RDT, while 12.2% (11/90) were seropositive to antibody binding by ELISA. Additionally, no correlation was found between cross-reactivity against the spike protein of SARS-CoV-2 and the HCoVs-OC43 and HcoVs-NL63 spikes. However, during the pandemic, 53.8% (21/39) of women sampled at delivery were seropositive by RDT, all women (28/28-100%) were seropositive by ELISA and 90% (20/22) of the samples from pregnant women tested for neutralization (20/22) had detectable neutralizing antibody responses during the COVID-19 pandemic. A transplacental transfer of binding antibodies from the mother to the child was found in 76.9% (30/39) of the tested dyads with a high prevalence during pandemic (26/28-86.7%) than prior the pandemic (4/11-13.3%).
Discussion: This study goes to reinforce the need for vaccination as though, all participants elicited a response towards endemic coronaviruses before the COVID-19 pandemic, a very small fraction of participants had binding antibodies which cross-react with SARS-CoV-2 and none of these were neutralizing. Anti-SARS-CoV-2 antibodies from the studied pregnant Cameroonian women at delivery during the pandemic had neutralizing activity against the founder variant and were efficiently transferred to the newborn. However the neutralization against other variants of concern warrants future investigation.
Keywords: COVID-19; SARS-CoV-2; neutralizing antibodies; pregnancy; transplacental antibody transfer.
. 2025 Nov 19:16:1628102.
doi: 10.3389/fimmu.2025.1628102. eCollection 2025. Transplacental transferred anti-SARS-CoV-2 neutralizing antibodies in unvaccinated pregnant women in Cameroon occurred during the COVID-19 pandemic, but not in the pre-pandemic period
Reine Medouen Ndeumou Seumko'o 1 2 3 , Thandeka Moyo-Gwete 4 5 , Tandile Hermanus 4 5 , Sosthene Hillary Matabou Tene 2 3 6 , Romeo Brice Dieffouo Djounda 2 3 6 , Chris Marco Mbianda Nana 1 2 , Bernard Marie Zambo Bitye 1 2 , Tekougang Berenice Kenfack Zangue 1 2 7 , Bodin Darcisse Kwanou Tchakounte 1 2 , Eitel Mpoudi Ngolle 3 , Diane Wallace Taylor 8 , Rose Gana Fomban Leke 2 3 , Rosette Megnekou 1 2 , Penny L Moore 4 5 9 10 , Livo Forgu Esemu 2 3 11
Affiliations
- PMID: 41346631
- PMCID: PMC12672500
- DOI: 10.3389/fimmu.2025.1628102
Introduction: Neutralizing antibodies (NAbs) are critical for protection against SARS-CoV-2, but there is limited information on their role in pregnancy, especially among Cameroonian women. Here, we aimed to determine the prevalence of pan-coronavirus reactive antibodies from pregnant women sampled before and during the COVID-19 pandemic.
Methods: Plasma samples from 629 women in the second trimester and 661 at delivery were collected pre-COVID-19 and from 39 women at delivery during COVID-19 in Yaoundé, Cameroon. All samples were screened using the Abbott Panbio™ COVID-19 rapid diagnostic test (RDT). Enzyme-linked immunosorbent assays (ELISA) and the spike-pseudotyped lentivirus neutralization assay were done to measure antibody binding and neutralizing capacity in 118 and 33 samples, respectively.
Results: Before the pandemic, 16.5% (213/1290) of pregnant women were seropositive for cross-reactive anti-SARS-CoV-2 antibodies by RDT, while 12.2% (11/90) were seropositive to antibody binding by ELISA. Additionally, no correlation was found between cross-reactivity against the spike protein of SARS-CoV-2 and the HCoVs-OC43 and HcoVs-NL63 spikes. However, during the pandemic, 53.8% (21/39) of women sampled at delivery were seropositive by RDT, all women (28/28-100%) were seropositive by ELISA and 90% (20/22) of the samples from pregnant women tested for neutralization (20/22) had detectable neutralizing antibody responses during the COVID-19 pandemic. A transplacental transfer of binding antibodies from the mother to the child was found in 76.9% (30/39) of the tested dyads with a high prevalence during pandemic (26/28-86.7%) than prior the pandemic (4/11-13.3%).
Discussion: This study goes to reinforce the need for vaccination as though, all participants elicited a response towards endemic coronaviruses before the COVID-19 pandemic, a very small fraction of participants had binding antibodies which cross-react with SARS-CoV-2 and none of these were neutralizing. Anti-SARS-CoV-2 antibodies from the studied pregnant Cameroonian women at delivery during the pandemic had neutralizing activity against the founder variant and were efficiently transferred to the newborn. However the neutralization against other variants of concern warrants future investigation.
Keywords: COVID-19; SARS-CoV-2; neutralizing antibodies; pregnancy; transplacental antibody transfer.