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J Virol Methods. 2014 Jul 24. pii: S0166-0934(14)00291-2. doi: 10.1016/j.jviromet.2014.07.020. [Epub ahead of print]
Influenza A/B virus detection and Influenza A virus subtyping with emphasis on the novel H7N9 virus by using multiplex real-time RT-PCR.
Kuo RL1, Yang SL2, Liu YC2, Chen LT2, Mok CK3, Kuo SM3, Shih SR1, Tsao KC4.
Author information
Abstract
Infections of the novel avian influenza A H7N9 virus cause severe respiratory diseases and death. In this study, to develop highly sensitive methods for differentially detecting the H7N9 virus, multiplex and singular real-time reverse transcription polymerase chain reaction (RT-PCR) assays were established and examined by targeting the H7 and N9 genes of the H7N9 virus. Furthermore, an additional multiplex assay combining previous real-time RT-PCR designs was established to subtype the pandemic H1N1, H3, and H5 influenza viruses. Applying the proposed assay system to analyze 100 clinical specimens collected from respiratory infection cases identified influenza A viruses (pandemic H1N1 and H3) in 23 samples. It has been demonstrated that other common respiratory viruses will not be detected by using this platform.
Copyright ? 2014. Published by Elsevier B.V.
KEYWORDS:
influenza A H7N9 virus; multiplex real-time reverse transcription polymerase chain reaction (RT-PCR); rapid and sensitive detection
PMID:
25066277
[PubMed - as supplied by publisher]
Influenza A/B virus detection and Influenza A virus subtyping with emphasis on the novel H7N9 virus by using multiplex real-time RT-PCR.
Kuo RL1, Yang SL2, Liu YC2, Chen LT2, Mok CK3, Kuo SM3, Shih SR1, Tsao KC4.
Author information
Abstract
Infections of the novel avian influenza A H7N9 virus cause severe respiratory diseases and death. In this study, to develop highly sensitive methods for differentially detecting the H7N9 virus, multiplex and singular real-time reverse transcription polymerase chain reaction (RT-PCR) assays were established and examined by targeting the H7 and N9 genes of the H7N9 virus. Furthermore, an additional multiplex assay combining previous real-time RT-PCR designs was established to subtype the pandemic H1N1, H3, and H5 influenza viruses. Applying the proposed assay system to analyze 100 clinical specimens collected from respiratory infection cases identified influenza A viruses (pandemic H1N1 and H3) in 23 samples. It has been demonstrated that other common respiratory viruses will not be detected by using this platform.
Copyright ? 2014. Published by Elsevier B.V.
KEYWORDS:
influenza A H7N9 virus; multiplex real-time reverse transcription polymerase chain reaction (RT-PCR); rapid and sensitive detection
PMID:
25066277
[PubMed - as supplied by publisher]
