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Vaccine. 2014 Jun 17. pii: S0264-410X(14)00805-6. doi: 10.1016/j.vaccine.2014.06.016. [Epub ahead of print]
A single dose of whole inactivated H7N9 influenza vaccine confers protection from severe disease but not infection in ferrets.
Wong SS1, Jeevan T1, Kercher L2, Yoon SW3, Petkova AM1, Crumpton JC1, Franks J1, Debeauchamp J1, Rubrum A1, Seiler P1, Krauss S1, Webster R1, Webby RJ4.
Author information
Abstract
The H7N9 influenza virus caused significant mortality and morbidity in infected humans during an outbreak in China in 2013 stimulating vaccine development efforts. As previous H7-based vaccines have been poorly immunogenic in humans we sought to determine the immunogenic and protective properties of an inactivated whole virus vaccine derived from a 2013 H7N9 virus in ferrets. As whole virus vaccine preparations have been shown to be more immunogenic in humans, but less likely to be used, than split or surface antigen formulations, we vaccinated ferrets with a single dose of 15, 30, or 50μg of the vaccine and subsequently challenged with wild-type A/Anhui/1/2013 (H7N9) either by direct instillation or by contact with infected animals. Although ferrets vaccinated with higher doses of vaccine had higher serum hemagglutinin inhibition (HI) titers, the titers were still low. During subsequent instillation challenge, however, ferrets vaccinated with 50μg of vaccine showed no illness and shed significantly less virus than mock vaccinated controls. All vaccinated ferrets had lower virus loads in their lungs as compared to controls. In a separate study where unvaccinated-infected ferrets were placed in the same cage with vaccinated-uninfected ferrets, vaccination did not prevent infection in the contact ferrets, although they showed a trend of lower viral load. Overall, we conclude that inactivated whole-virus H7N9 vaccine was able to reduce the severity of infection and viral load, despite the lack of hemagglutinin-inhibiting antibodies.
Copyright ? 2014. Published by Elsevier Ltd.
KEYWORDS:
Ferrets; H7N9; Vaccine
PMID:
24950355
[PubMed - as supplied by publisher]
A single dose of whole inactivated H7N9 influenza vaccine confers protection from severe disease but not infection in ferrets.
Wong SS1, Jeevan T1, Kercher L2, Yoon SW3, Petkova AM1, Crumpton JC1, Franks J1, Debeauchamp J1, Rubrum A1, Seiler P1, Krauss S1, Webster R1, Webby RJ4.
Author information
Abstract
The H7N9 influenza virus caused significant mortality and morbidity in infected humans during an outbreak in China in 2013 stimulating vaccine development efforts. As previous H7-based vaccines have been poorly immunogenic in humans we sought to determine the immunogenic and protective properties of an inactivated whole virus vaccine derived from a 2013 H7N9 virus in ferrets. As whole virus vaccine preparations have been shown to be more immunogenic in humans, but less likely to be used, than split or surface antigen formulations, we vaccinated ferrets with a single dose of 15, 30, or 50μg of the vaccine and subsequently challenged with wild-type A/Anhui/1/2013 (H7N9) either by direct instillation or by contact with infected animals. Although ferrets vaccinated with higher doses of vaccine had higher serum hemagglutinin inhibition (HI) titers, the titers were still low. During subsequent instillation challenge, however, ferrets vaccinated with 50μg of vaccine showed no illness and shed significantly less virus than mock vaccinated controls. All vaccinated ferrets had lower virus loads in their lungs as compared to controls. In a separate study where unvaccinated-infected ferrets were placed in the same cage with vaccinated-uninfected ferrets, vaccination did not prevent infection in the contact ferrets, although they showed a trend of lower viral load. Overall, we conclude that inactivated whole-virus H7N9 vaccine was able to reduce the severity of infection and viral load, despite the lack of hemagglutinin-inhibiting antibodies.
Copyright ? 2014. Published by Elsevier Ltd.
KEYWORDS:
Ferrets; H7N9; Vaccine
PMID:
24950355
[PubMed - as supplied by publisher]
