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J Clin Bioinforma. 2015 Mar 3;5:2. doi: 10.1186/s13336-015-0017-5. eCollection 2015.
Variations in genome-wide RNAi screens: lessons from influenza research.
Chou YC1, Lai MM2, Wu YC3, Hsu NC3, Jeng KS1, Su WC4.
Author information
Abstract
Genome-wide RNA interference (RNAi) screening is an emerging and powerful technique for genetic screens, which can be divided into arrayed RNAi screen and pooled RNAi screen/selection based on different screening strategies. To date, several genome-wide RNAi screens have been successfully performed to identify host factors essential for influenza virus replication. However, the host factors identified by different research groups are not always consistent. Taking influenza virus screens as an example, we found that a number of screening parameters may directly or indirectly influence the primary hits identified by the screens. This review highlights the differences among the published genome-wide screening approaches and offers recommendations for performing a good pooled shRNA screen/selection.
KEYWORDS:
Arrayed siRNA screen; Genome-wide screen; Influenza virus; Lentivirus; Pooled shRNA screen; RNA interference (RNAi)
PMID: 25745555 [PubMed] PMCID: PMC4350949 Free PMC Article
Variations in genome-wide RNAi screens: lessons from influenza research.
Chou YC1, Lai MM2, Wu YC3, Hsu NC3, Jeng KS1, Su WC4.
Author information
Abstract
Genome-wide RNA interference (RNAi) screening is an emerging and powerful technique for genetic screens, which can be divided into arrayed RNAi screen and pooled RNAi screen/selection based on different screening strategies. To date, several genome-wide RNAi screens have been successfully performed to identify host factors essential for influenza virus replication. However, the host factors identified by different research groups are not always consistent. Taking influenza virus screens as an example, we found that a number of screening parameters may directly or indirectly influence the primary hits identified by the screens. This review highlights the differences among the published genome-wide screening approaches and offers recommendations for performing a good pooled shRNA screen/selection.
KEYWORDS:
Arrayed siRNA screen; Genome-wide screen; Influenza virus; Lentivirus; Pooled shRNA screen; RNA interference (RNAi)
PMID: 25745555 [PubMed] PMCID: PMC4350949 Free PMC Article