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June 15 2023
https://doi.org/10.1083/jcb.202306020
Annika Kratzel, Volker Thiel
Replication of the coronaviral genome during the virus life cycle results in the production and release of new infectious virus particles (1). SARS-CoV-2 binds to its cellular receptor angiotensin-converting enzyme 2 (ACE2), and assisted by cell surface proteases, the virus and cell membranes fuse to enable the viral genome to enter the host cell cytoplasm. Here, the viral genome is directly translated to produce the viral proteins that make up the replication and transcription complex (RTC), namely the non-structural proteins (NSPs) 1–16 (2). By a not yet well understood process, the NSPs 2–16 engage host cell ER membranes to form the coronavirus replication organelles, e.g., perinuclear double membrane vesicles (DMVs), where viral RNA replication and transcription takes place. It is known that NSPs 3, 4, and 6 are involved in the formation of DMVs (3), but exactly how the virus can hijack the host ER membranes, and which host cell proteins may play a role, remains elusive. ...
https://doi.org/10.1083/jcb.202306020
Annika Kratzel, Volker Thiel
Replication of the coronaviral genome during the virus life cycle results in the production and release of new infectious virus particles (1). SARS-CoV-2 binds to its cellular receptor angiotensin-converting enzyme 2 (ACE2), and assisted by cell surface proteases, the virus and cell membranes fuse to enable the viral genome to enter the host cell cytoplasm. Here, the viral genome is directly translated to produce the viral proteins that make up the replication and transcription complex (RTC), namely the non-structural proteins (NSPs) 1–16 (2). By a not yet well understood process, the NSPs 2–16 engage host cell ER membranes to form the coronavirus replication organelles, e.g., perinuclear double membrane vesicles (DMVs), where viral RNA replication and transcription takes place. It is known that NSPs 3, 4, and 6 are involved in the formation of DMVs (3), but exactly how the virus can hijack the host ER membranes, and which host cell proteins may play a role, remains elusive. ...