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Emerg Infect Dis . Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2

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    Emerg Infect Dis . Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2


    Emerg Infect Dis


    . 2020 Jul 1;26(10).
    doi: 10.3201/eid2610.201800. Online ahead of print.
    Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2

    Clinton R Paden, Ying Tao, Krista Queen, Jing Zhang, Yan Li, Anna Uehara, Suxiang TongFree article

    Abstract

    We describe validated protocols for generating high-quality, full-length severe acute respiratory syndrome coronavirus 2 genomes from primary samples. One protocol uses multiplex reverse transcription PCR, followed by MinION or MiSeq sequencing; the other uses singleplex, nested reverse transcription PCR and Sanger sequencing. These protocols enable sensitive virus sequencing in different laboratory environments.

    Keywords: COVID-19; SARS-CoV-2; coronavirus; coronavirus disease; genomics; high-throughput nucleotide sequencing; respiratory infections; severe acute respiratory syndrome coronavirus 2; viruses; whole-genome sequencing; zoonoses.

    Tags: None
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