Tweet
1 Jan 2025
DOI: 10.1126/sciadv.adr6140
KELLY N. MESSINGHAM, PAIGE T. RICHARDS, ANTHONY FLECK, RADHIKA A. PATEL, MARIJA DJURKOVIC, JONAH ELLIFF, SAMUEL CONNEL , TYLER P. CROWE, JUAN MUNOZ GONZALEZ, FRANCOISE GOURRONC, JACOB A. DILLARD, ROBERT A. DAVEY, ALOYSIUS KLINGELHUTZ, OLENA SHTANKO, AND WENDY MAURY
Abstract
Ebola virus (EBOV) causes severe human disease. During late infection, EBOV virions are on the skin’s surface; however, the permissive skin cell types and the route of virus translocation to the epidermal surface are unknown. We describe a human skin explant model and demonstrate that EBOV infection of human skin via basal media increases in a time-dependent and dose-dependent manner. In the dermis, cells of myeloid, endothelial, and fibroblast origin were EBOV antigen–positive whereas keratinocytes harbored virus in the epidermis. Infectious virus was detected on the apical epidermal surface within 3 days, indicating that virus propagates and traffics through the explants. Purified human fibroblasts and keratinocytes supported EBOV infection ex vivo and both cell types required the phosphatidylserine receptor, AXL, and the endosomal protein, NPC1, for virus entry. This platform identified susceptible cell types and demonstrated dynamic trafficking of EBOV virions. These findings may explain person-to-person transmission via skin contact.
DOI: 10.1126/sciadv.adr6140
KELLY N. MESSINGHAM, PAIGE T. RICHARDS, ANTHONY FLECK, RADHIKA A. PATEL, MARIJA DJURKOVIC, JONAH ELLIFF, SAMUEL CONNEL , TYLER P. CROWE, JUAN MUNOZ GONZALEZ, FRANCOISE GOURRONC, JACOB A. DILLARD, ROBERT A. DAVEY, ALOYSIUS KLINGELHUTZ, OLENA SHTANKO, AND WENDY MAURY
Abstract
Ebola virus (EBOV) causes severe human disease. During late infection, EBOV virions are on the skin’s surface; however, the permissive skin cell types and the route of virus translocation to the epidermal surface are unknown. We describe a human skin explant model and demonstrate that EBOV infection of human skin via basal media increases in a time-dependent and dose-dependent manner. In the dermis, cells of myeloid, endothelial, and fibroblast origin were EBOV antigen–positive whereas keratinocytes harbored virus in the epidermis. Infectious virus was detected on the apical epidermal surface within 3 days, indicating that virus propagates and traffics through the explants. Purified human fibroblasts and keratinocytes supported EBOV infection ex vivo and both cell types required the phosphatidylserine receptor, AXL, and the endosomal protein, NPC1, for virus entry. This platform identified susceptible cell types and demonstrated dynamic trafficking of EBOV virions. These findings may explain person-to-person transmission via skin contact.
