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Virol J
. 2024 Jul 11;21(1):156.
doi: 10.1186/s12985-024-02430-x. Evaluation of the FPMC respiratory panel for detection of respiratory tract pathogens in nasopharyngeal swab and sputum specimens
Li Xue 1 , Jianhong Zhu 1 , Ke Lei 1 , Zeshi Liu 1 , Yiwei Tang 2 , Bo Zhong 3 , Ning Gao 1 , Chaoliang Xiong 1 , Jing Lei 1 , Ying Tian 1 , Weixiao Zhou 1 , Nan Feng 1 , Xue Zhang 1 , Dong Chen 1 , Jing Li 1 , Yan Geng 4
Affiliations
Objectives: The performance of the new Respiratory Pathogen panel (fluorescent probe melting curve, FPMC) for the qualitative detection of 12 organisms (chlamydia pneumoniae, mycoplasma pneumoniae, adenovirus, influenza A virus, influenza B virus, parainfluenza virus, rhinovirus, etc.) was assessed.
Methods: Prospectively collected nasopharyngeal swab (NPS) and sputum specimens (n = 635) were detected by using the FPMC panel, with the Sanger sequencing method as the comparative method.
Results: The overall percent concordance between the FPMC analysis method and the Sanger sequencing method was 100% and 99.66% for NPS and sputum specimens, respectively. The FPMC testified an overall positive percent concordance of 100% for both NPS and sputum specimens. The FPMC analysis method also testified an overall negative percent concordance of 100% and 99.38% for NPS and sputum specimens, respectively.
Conclusions: The FPMC analysis method is a stable and accurate assay for rapid, comprehensive detecting for respiratory pathogens.
Keywords: Fluorescent probe melting curve PCR; Molecular detection; Respiratory pathogen.
. 2024 Jul 11;21(1):156.
doi: 10.1186/s12985-024-02430-x. Evaluation of the FPMC respiratory panel for detection of respiratory tract pathogens in nasopharyngeal swab and sputum specimens
Li Xue 1 , Jianhong Zhu 1 , Ke Lei 1 , Zeshi Liu 1 , Yiwei Tang 2 , Bo Zhong 3 , Ning Gao 1 , Chaoliang Xiong 1 , Jing Lei 1 , Ying Tian 1 , Weixiao Zhou 1 , Nan Feng 1 , Xue Zhang 1 , Dong Chen 1 , Jing Li 1 , Yan Geng 4
Affiliations
- PMID: 38992721
- DOI: 10.1186/s12985-024-02430-x
Objectives: The performance of the new Respiratory Pathogen panel (fluorescent probe melting curve, FPMC) for the qualitative detection of 12 organisms (chlamydia pneumoniae, mycoplasma pneumoniae, adenovirus, influenza A virus, influenza B virus, parainfluenza virus, rhinovirus, etc.) was assessed.
Methods: Prospectively collected nasopharyngeal swab (NPS) and sputum specimens (n = 635) were detected by using the FPMC panel, with the Sanger sequencing method as the comparative method.
Results: The overall percent concordance between the FPMC analysis method and the Sanger sequencing method was 100% and 99.66% for NPS and sputum specimens, respectively. The FPMC testified an overall positive percent concordance of 100% for both NPS and sputum specimens. The FPMC analysis method also testified an overall negative percent concordance of 100% and 99.38% for NPS and sputum specimens, respectively.
Conclusions: The FPMC analysis method is a stable and accurate assay for rapid, comprehensive detecting for respiratory pathogens.
Keywords: Fluorescent probe melting curve PCR; Molecular detection; Respiratory pathogen.