Anal Bioanal Chem . CRISPR/Cas13a combined with hybridization chain reaction for visual detection of influenza A (H1N1) virus

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Anal Bioanal Chem


. 2022 Oct 20.
doi: 10.1007/s00216-022-04380-1. Online ahead of print.
CRISPR/Cas13a combined with hybridization chain reaction for visual detection of influenza A (H1N1) virus


Hongyu Zhou ^{1 2} , Shengjun Bu ² , Yao Xu ² , Lulu Xue ² , Zhongyi Li ² , Zhuo Hao ² , Jiayu Wan ³ , Feng Tang ⁴



Affiliations

• PMID: 36264297
• DOI: 10.1007/s00216-022-04380-1

Abstract

This study provides proof of concept of a colorimetric biosensor for influenza H1N1 virus assay based on the CRISPR/Cas13a system and hybridization chain reaction (HCR). Target RNA of influenza H1N1 virus activated the trans-cleavage activity of Cas13a, which cleaved the special RNA sequence (-UUU-) of the probe, further initiating HCR to copiously generate G-rich DNA. Abundant G-quadruplex/hemin was formed in the presence of hemin, thus catalyzing a colorimetric reaction. The colorimetric biosensor exhibited a linear relationship from 10 pM to 100 nM. The detection limit was 0.152 pM. The biosensor specificity was excellent. This new and sensitive detection method for influenza virus is a promising rapid influenza diagnostic test.

Keywords: CRISPR/Cas13a; DNAzyme; Hybridization chain reaction; Influenza H1N1 virus detection.