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J Clin Virol. 2017 Dec 13;99-100:5-9. doi: 10.1016/j.jcv.2017.12.005. [Epub ahead of print]
Diagnostic performances of the Xpert? Flu PCR test and the OSOM? immunochromatographic rapid test for influenza A and B virus among adult patients in the Emergency Department.
Antoniol S1, Fidouh N2, Ghazali A1, Ichou H2, Bouzid D1, Kenway P3, Choquet C3, Visseaux B2, Casalino E4; Emergency Department study group on respiratory viruses.
Author information
Abstract
BACKGROUND:
New rapid influenza diagnostic tests (RIDT) are available but their clinical utility in adults has not been validated.
OBJECTIVES:
To evaluate the diagnostic performances of OSOM? Ultra Flu A&B a RIDT on viral strains of influenza A/B from the last epidemic season, and its feasibility by Emergency Department (ED) physicians and nurses.
STUDY DESIGN:
Of the 1099 patients admitted to the ED with suspected influenza, all having a nasopharyngeal swab tested by the Xpert? Flu PCR and then stored at -20 ?C; 500 were selected at random and their samples were tested using the RIDT. Two experts reviewed ED and hospital medical records and all virological data to define influenza cases. Intra- and inter-observer variability were calculated.
RESULTS:
Of the 500 patients included 45% were ≥75 years, 122 (24.4%) presented with influenza based on clinical and virological criteria. PCR test performances (%) were Se 98.4 (95% CI 93.6-99.7), Spe 99.7 (98.3-100), PPV 99.2 (94.8-100) and NPV 99.5 (97.9-100); and RIDT performances were Se 95.1 (89.2-97.9), Spe 98.4 (96.4-99.4), PPV 95.1 (87.2-99.9) and NPV 98.4 (96.4-98). There was no difference in test performance between influenza A and B virus nor between the influenza A subtypes. Intra- and inter-observer variability of RIDT were 0.94 (0.89-0.99) and 0.96 (0.92-1).
CONCLUSION:
Our results show that the Xpert? Flu PCR and the OSOM? Ultra Flu A&B Test perform very well in diagnosing strains of circulating virus in adults and elderly. Our results also confirm the feasibility of this RIDT at point-of-care by ED staff.
Copyright ? 2017. Published by Elsevier B.V.
KEYWORDS:
Adults; Diagnostic performance; Elderly; Emergency department; PCR influenza; Rapid influenza diagnostic test
PMID: 29257982 DOI: 10.1016/j.jcv.2017.12.005
Diagnostic performances of the Xpert? Flu PCR test and the OSOM? immunochromatographic rapid test for influenza A and B virus among adult patients in the Emergency Department.
Antoniol S1, Fidouh N2, Ghazali A1, Ichou H2, Bouzid D1, Kenway P3, Choquet C3, Visseaux B2, Casalino E4; Emergency Department study group on respiratory viruses.
Author information
Abstract
BACKGROUND:
New rapid influenza diagnostic tests (RIDT) are available but their clinical utility in adults has not been validated.
OBJECTIVES:
To evaluate the diagnostic performances of OSOM? Ultra Flu A&B a RIDT on viral strains of influenza A/B from the last epidemic season, and its feasibility by Emergency Department (ED) physicians and nurses.
STUDY DESIGN:
Of the 1099 patients admitted to the ED with suspected influenza, all having a nasopharyngeal swab tested by the Xpert? Flu PCR and then stored at -20 ?C; 500 were selected at random and their samples were tested using the RIDT. Two experts reviewed ED and hospital medical records and all virological data to define influenza cases. Intra- and inter-observer variability were calculated.
RESULTS:
Of the 500 patients included 45% were ≥75 years, 122 (24.4%) presented with influenza based on clinical and virological criteria. PCR test performances (%) were Se 98.4 (95% CI 93.6-99.7), Spe 99.7 (98.3-100), PPV 99.2 (94.8-100) and NPV 99.5 (97.9-100); and RIDT performances were Se 95.1 (89.2-97.9), Spe 98.4 (96.4-99.4), PPV 95.1 (87.2-99.9) and NPV 98.4 (96.4-98). There was no difference in test performance between influenza A and B virus nor between the influenza A subtypes. Intra- and inter-observer variability of RIDT were 0.94 (0.89-0.99) and 0.96 (0.92-1).
CONCLUSION:
Our results show that the Xpert? Flu PCR and the OSOM? Ultra Flu A&B Test perform very well in diagnosing strains of circulating virus in adults and elderly. Our results also confirm the feasibility of this RIDT at point-of-care by ED staff.
Copyright ? 2017. Published by Elsevier B.V.
KEYWORDS:
Adults; Diagnostic performance; Elderly; Emergency department; PCR influenza; Rapid influenza diagnostic test
PMID: 29257982 DOI: 10.1016/j.jcv.2017.12.005