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Direct Detection of Influenza A/B in Less Than Twenty Minutes Using a Commercially-Available Rapid PCR Assay

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  • Direct Detection of Influenza A/B in Less Than Twenty Minutes Using a Commercially-Available Rapid PCR Assay

    J Clin Microbiol. 2015 Apr 29. pii: JCM.00791-15. [Epub ahead of print]
    Direct Detection of Influenza A/B in Less Than Twenty Minutes Using a Commercially-Available Rapid PCR Assay.

    Binnicker MJ1, Espy MJ2, Irish CL2, Vetter EA2.
    Author information

    Abstract

    We compared a FDA-cleared, rapid (<20 min) PCR assay (Roche cobas? Liat) to our routine influenza A/B real-time PCR (Focus Simplexa? Flu A/B & RSV Direct) using respiratory swabs (n=197). The cobas Liat influenza A and B assays demonstrated sensitivities of 99.2% (123/124) and 100% (23/23), respectively, while showing a specificity of 100% for both targets.
    Copyright ? 2015, American Society for Microbiology. All Rights Reserved.


    PMID: 25926484 [PubMed - as supplied by publisher]
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