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J Clin Microbiol. 2014 Oct 15. pii: JCM.02024-14. [Epub ahead of print]
Performance of Rapid Influenza Diagnostic Test in an outbreak setting.
Peci A1, Winter AL1, King EC1, Blair J1, Gubbay JB2.
Author information
Abstract
BACKGROUND:
Rapid Influenza Diagnostic Tests (RIDTs) may be useful during institutional respiratory outbreaks to identify influenza and enable antivirals to be rapidly administered to cases and prophylax those exposed to the virus but not yet symptomatic. Performance of RIDTs at the outbreak level is not well documented in the literature.
OBJECTIVE:
This study aims to evaluate performance of RIDTs in comparison with real-time reverse transcription PCR (rRT-PCR) in the context of institutional respiratory outbreaks.
METHODS:
This study included outbreak related respiratory specimens tested for influenza at Public Health Ontario Laboratory by both RIDT and rRT-PCR, from September 1, 2010 to April 30, 2013.
RESULTS:
At the outbreak level, performance testing of RIDTs as compared to rRT-PCR for detection of any influenza type demonstrated an overall sensitivity of 76.5%, specificity of 99.7%, PPV of 99.5% and a NPV of 85.3%.
CONCLUSIONS:
Due to high specificity, RIDTs can play a role in screening for influenza in outbreaks and instituting antiviral therapy in a timely manner when positive. RIDTs can also be useful in remote settings where molecular virology testing is not easily accessible. Suboptimal sensitivity of RIDTs can be addressed by use of molecular testing.
Copyright ? 2014, American Society for Microbiology. All Rights Reserved.
PMID:
25320225
[PubMed - as supplied by publisher]
Performance of Rapid Influenza Diagnostic Test in an outbreak setting.
Peci A1, Winter AL1, King EC1, Blair J1, Gubbay JB2.
Author information
Abstract
BACKGROUND:
Rapid Influenza Diagnostic Tests (RIDTs) may be useful during institutional respiratory outbreaks to identify influenza and enable antivirals to be rapidly administered to cases and prophylax those exposed to the virus but not yet symptomatic. Performance of RIDTs at the outbreak level is not well documented in the literature.
OBJECTIVE:
This study aims to evaluate performance of RIDTs in comparison with real-time reverse transcription PCR (rRT-PCR) in the context of institutional respiratory outbreaks.
METHODS:
This study included outbreak related respiratory specimens tested for influenza at Public Health Ontario Laboratory by both RIDT and rRT-PCR, from September 1, 2010 to April 30, 2013.
RESULTS:
At the outbreak level, performance testing of RIDTs as compared to rRT-PCR for detection of any influenza type demonstrated an overall sensitivity of 76.5%, specificity of 99.7%, PPV of 99.5% and a NPV of 85.3%.
CONCLUSIONS:
Due to high specificity, RIDTs can play a role in screening for influenza in outbreaks and instituting antiviral therapy in a timely manner when positive. RIDTs can also be useful in remote settings where molecular virology testing is not easily accessible. Suboptimal sensitivity of RIDTs can be addressed by use of molecular testing.
Copyright ? 2014, American Society for Microbiology. All Rights Reserved.
PMID:
25320225
[PubMed - as supplied by publisher]
