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J Clin Microbiol. 2013 Mar 13. [Epub ahead of print]
Comparison of Four Multiplex Assays for the Detection of Respiratory Viruses: Biofire FilmArray RP, Genmark eSensor RVP, Luminex xTAG RVPv1 and Luminex xTAG RVP FAST.
Popowitch EB, O'Neill SS, Miller MB.
Source
University of North Carolina Health Care System, Chapel Hill, NC.
Abstract
There are several FDA-cleared molecular respiratory virus panels available today, each with advantages and disadvantages. This study compares four multiplex panels: BioFire Diagnostics FilmArray RP, GenMark Dx eSensor RVP, Luminex xTAG RVPv1 and the Luminex xTAG RVP FAST. Three hundred specimens (200 retrospective and 100 consecutive) were tested by all four platforms to determine performance characteristics. Overall sensitivity and specificity for each panel was as follows: FilmArray RP 84.5% (95% CI: 79.2, 88.6), 100% (96.2, 100); eSensor RVP 98.3% (95.5, 99.5), 99.2% (95.4, 100); Luminex RVPv1 92.7% (88.5, 95.4), 99.8% (96.0, 100); RVP FAST 84.4% (78.5, 88.9), 99.9% (96.1, 100). The sensitivity of each assay fluctuated by viral target with the greatest discrepancies noted for adenovirus and influenza B detection. Hands-on time and time to result was recorded, and ease of use was assessed to generate a complete profile of each assay.
PMID:
23486707
[PubMed - as supplied by publisher]
Comparison of Four Multiplex Assays for the Detection of Respiratory Viruses: Biofire FilmArray RP, Genmark eSensor RVP, Luminex xTAG RVPv1 and Luminex xTAG RVP FAST.
Popowitch EB, O'Neill SS, Miller MB.
Source
University of North Carolina Health Care System, Chapel Hill, NC.
Abstract
There are several FDA-cleared molecular respiratory virus panels available today, each with advantages and disadvantages. This study compares four multiplex panels: BioFire Diagnostics FilmArray RP, GenMark Dx eSensor RVP, Luminex xTAG RVPv1 and the Luminex xTAG RVP FAST. Three hundred specimens (200 retrospective and 100 consecutive) were tested by all four platforms to determine performance characteristics. Overall sensitivity and specificity for each panel was as follows: FilmArray RP 84.5% (95% CI: 79.2, 88.6), 100% (96.2, 100); eSensor RVP 98.3% (95.5, 99.5), 99.2% (95.4, 100); Luminex RVPv1 92.7% (88.5, 95.4), 99.8% (96.0, 100); RVP FAST 84.4% (78.5, 88.9), 99.9% (96.1, 100). The sensitivity of each assay fluctuated by viral target with the greatest discrepancies noted for adenovirus and influenza B detection. Hands-on time and time to result was recorded, and ease of use was assessed to generate a complete profile of each assay.
PMID:
23486707
[PubMed - as supplied by publisher]
