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Anal Biochem. 2012 Oct 24. pii: S0003-2697(12)00536-2. doi: 10.1016/j.ab.2012.10.025. [Epub ahead of print]
Fluorescent primer-based in vitro transcription system of viral RNA-dependent RNA polymerases.
Wang Q, Weng L, Jiang H, Zhang S, Toyoda T.
Source
Unit of Viral Genome Regulation, the Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200025, P. R. China.
Abstract
Viral infection is a leading cause of disease and death. Although vaccines are the most effective method of controlling viral infections, antiviral drugs are also important. Here, we established an in vitro transcription system by using fluorescein isothiocyanate-conjugated primers for RNA polymerases of viruses which are important disease-causing human pathogens (influenza, hepatitis C, Japanese encephalitis viruses, and enterovirus 71). This technology will allow us to analyze RNA polymerase activity without using radioisotopes.
Copyright ? 2012. Published by Elsevier Inc.
PMID:
23103398
[PubMed - as supplied by publisher]
Fluorescent primer-based in vitro transcription system of viral RNA-dependent RNA polymerases.
Wang Q, Weng L, Jiang H, Zhang S, Toyoda T.
Source
Unit of Viral Genome Regulation, the Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200025, P. R. China.
Abstract
Viral infection is a leading cause of disease and death. Although vaccines are the most effective method of controlling viral infections, antiviral drugs are also important. Here, we established an in vitro transcription system by using fluorescein isothiocyanate-conjugated primers for RNA polymerases of viruses which are important disease-causing human pathogens (influenza, hepatitis C, Japanese encephalitis viruses, and enterovirus 71). This technology will allow us to analyze RNA polymerase activity without using radioisotopes.
Copyright ? 2012. Published by Elsevier Inc.
PMID:
23103398
[PubMed - as supplied by publisher]
