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Nano Lett. 2011 Aug 17. [Epub ahead of print]
Silicon nanowire-based CMOS-compatible field-effect transistor nanosensors for ultrasensitive electrical detection of nucleic acids.
Gao A, Lu N, Dai P, Li T, Pei H, Gao X, Gong Y, Wang Y, Fan C.
Abstract
We herein report the design a novel semiconducting silicon nanowire field-effect transistor (SiNW-FET) biosensor array for ultrasensitive, label-free and real-time detection of nucleic acids. Highly responsive SiNWs with narrow sizes and high surface-to-volume-ratios were "top-down" fabricated with a complementary metal oxide semiconductor (CMOS) compatible anisotropic self-stop etching technique. When SiNWs were covalently modified with DNA probes, the nanosensor showed highly sensitive concentration-dependent conductance change in response to specific target DNA sequences. This SiNW-FET nanosensor revealed ultrahigh sensitivity for rapid and reliable detection of 1 fM of target DNA and high specificity single-nucleotide polymorphism (SNP) discrimination. As a proof-of-concept for multiplex detection with this small-size and mass producible sensor array, we demonstrated simultaneous selective detection of two pathogenic strain virus DNA (H1N1 and H5N1) of avian influenza.
PMID:
21848308
[PubMed - as supplied by publisher]
Silicon nanowire-based CMOS-compatible field-effect transistor nanosensors for ultrasensitive electrical detection of nucleic acids.
Gao A, Lu N, Dai P, Li T, Pei H, Gao X, Gong Y, Wang Y, Fan C.
Abstract
We herein report the design a novel semiconducting silicon nanowire field-effect transistor (SiNW-FET) biosensor array for ultrasensitive, label-free and real-time detection of nucleic acids. Highly responsive SiNWs with narrow sizes and high surface-to-volume-ratios were "top-down" fabricated with a complementary metal oxide semiconductor (CMOS) compatible anisotropic self-stop etching technique. When SiNWs were covalently modified with DNA probes, the nanosensor showed highly sensitive concentration-dependent conductance change in response to specific target DNA sequences. This SiNW-FET nanosensor revealed ultrahigh sensitivity for rapid and reliable detection of 1 fM of target DNA and high specificity single-nucleotide polymorphism (SNP) discrimination. As a proof-of-concept for multiplex detection with this small-size and mass producible sensor array, we demonstrated simultaneous selective detection of two pathogenic strain virus DNA (H1N1 and H5N1) of avian influenza.
PMID:
21848308
[PubMed - as supplied by publisher]
