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Clin Vaccine Immunol. 2014 May 28. pii: CVI.00129-14. [Epub ahead of print]
IgM, IgG, and IgA antibody responses to influenza A(H1N1)pdm09 hemagglutinin in infected persons during the first wave of the 2009 pandemic in the United States.
Li ZN1, Lin SC2, Carney PJ3, Li J3, Liu F3, Lu X3, Liu M2, Stevens J3, Levine M3, Katz JM3, Hancock K3.
Author information
Abstract
Novel influenza A(H1N1)pdm09 virus caused an influenza pandemic in 2009. IgM, IgG, and IgA antibody responses to A(H1N1)pdm09 hemagglutinin (HA) following A(H1N1)pdm09 virus infection were analyzed to understand antibody isotype responses. Age-matched control sera collected from U.S. residents in 2007 and 2008 were used to establish baseline levels of cross-reactive antibodies. IgM responses often used as an indicator of primary virus infection were mainly detected in young patient groups (≤5 yrs and 6-15 yrs), not in older age group, despite the genetic and antigenic differences between the HA of A(H1N1)pdm09 virus and pre-2009 seasonal H1N1 viruses. IgG and IgA responses to A(H1N1)pdm09 HA were detected in all age groups of infected persons. In persons aged 17-80 years, paired acute and convalescent serum samples demonstrated a four-fold or greater increase in the IgG and IgA responses to A(H1N1)pdm09 HA in 80% and 67% of A(H1N1)pdm09 virus-infected persons, respectively. The IgG antibody response to A(H1N1)pdm09 HA was cross-reactive with HAs from H1, H3, H5, and H13 subtypes suggesting that infections with subtypes other than A(H1N1)pdm09 could result in false positives by ELISA. Lower sensitivity compared to hemagglutination inhibition and microneutralization assays and the detection of cross-reactive antibodies against homologous and heterologous subtype are major drawback for application of ELISA in influenza serologic studies.
Copyright ? 2014, American Society for Microbiology. All Rights Reserved.
PMID:
24872516
[PubMed - as supplied by publisher]
IgM, IgG, and IgA antibody responses to influenza A(H1N1)pdm09 hemagglutinin in infected persons during the first wave of the 2009 pandemic in the United States.
Li ZN1, Lin SC2, Carney PJ3, Li J3, Liu F3, Lu X3, Liu M2, Stevens J3, Levine M3, Katz JM3, Hancock K3.
Author information
Abstract
Novel influenza A(H1N1)pdm09 virus caused an influenza pandemic in 2009. IgM, IgG, and IgA antibody responses to A(H1N1)pdm09 hemagglutinin (HA) following A(H1N1)pdm09 virus infection were analyzed to understand antibody isotype responses. Age-matched control sera collected from U.S. residents in 2007 and 2008 were used to establish baseline levels of cross-reactive antibodies. IgM responses often used as an indicator of primary virus infection were mainly detected in young patient groups (≤5 yrs and 6-15 yrs), not in older age group, despite the genetic and antigenic differences between the HA of A(H1N1)pdm09 virus and pre-2009 seasonal H1N1 viruses. IgG and IgA responses to A(H1N1)pdm09 HA were detected in all age groups of infected persons. In persons aged 17-80 years, paired acute and convalescent serum samples demonstrated a four-fold or greater increase in the IgG and IgA responses to A(H1N1)pdm09 HA in 80% and 67% of A(H1N1)pdm09 virus-infected persons, respectively. The IgG antibody response to A(H1N1)pdm09 HA was cross-reactive with HAs from H1, H3, H5, and H13 subtypes suggesting that infections with subtypes other than A(H1N1)pdm09 could result in false positives by ELISA. Lower sensitivity compared to hemagglutination inhibition and microneutralization assays and the detection of cross-reactive antibodies against homologous and heterologous subtype are major drawback for application of ELISA in influenza serologic studies.
Copyright ? 2014, American Society for Microbiology. All Rights Reserved.
PMID:
24872516
[PubMed - as supplied by publisher]
