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Influenza Other Respir Viruses. 2019 Jun 11. doi: 10.1111/irv.12661. [Epub ahead of print]
Detection of oseltamivir-resistant zoonotic and animal influenza A viruses using the rapid influenza antiviral resistance test.
Hodges EN1,2, Mishin VP1, De la Cruz J1,3, Guo Z1, Nguyen HT1,3, Fallows E4, Stevens J1, Wentworth DE1, Davis CT1, Gubareva LV1.
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Abstract
Mutations in the influenza virus neuraminidase (NA) that cause reduced susceptibility to the NA inhibitor (NAI) oseltamivir may occur naturally or following antiviral treatment. Currently, detection uses either a traditional NA inhibition assay or gene sequencing to identify known markers associated with reduced inhibition by oseltamivir. Both methods are laborious and require trained personnel. The influenza antiviral resistance test (iART), a prototype system developed by Becton, Dickinson and Company for research use only, offers a rapid and simple method to identify such viruses. This study investigated application of iART to influenza A viruses isolated from non-human hosts with a variety of NA subtypes (N1-N9).
? 2019 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd.
KEYWORDS:
antiviral drugs; avian; influenza A virus; resistance; zoonotic
PMID: 31187572 DOI: 10.1111/irv.12661
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Detection of oseltamivir-resistant zoonotic and animal influenza A viruses using the rapid influenza antiviral resistance test.
Hodges EN1,2, Mishin VP1, De la Cruz J1,3, Guo Z1, Nguyen HT1,3, Fallows E4, Stevens J1, Wentworth DE1, Davis CT1, Gubareva LV1.
Author information
Abstract
Mutations in the influenza virus neuraminidase (NA) that cause reduced susceptibility to the NA inhibitor (NAI) oseltamivir may occur naturally or following antiviral treatment. Currently, detection uses either a traditional NA inhibition assay or gene sequencing to identify known markers associated with reduced inhibition by oseltamivir. Both methods are laborious and require trained personnel. The influenza antiviral resistance test (iART), a prototype system developed by Becton, Dickinson and Company for research use only, offers a rapid and simple method to identify such viruses. This study investigated application of iART to influenza A viruses isolated from non-human hosts with a variety of NA subtypes (N1-N9).
? 2019 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd.
KEYWORDS:
antiviral drugs; avian; influenza A virus; resistance; zoonotic
PMID: 31187572 DOI: 10.1111/irv.12661
Free full text