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J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Nov 1;944C:1-5. doi: 10.1016/j.jchromb.2013.10.027. [Epub ahead of print]
Quantification of peramivir in dog plasma by liquid chromatography/tandem mass spectrometry employing precolumn derivatization.
Li X, Li Y, Wang J, Wang L, Zhong W, Ruan J, Zhang Z.
Source
Department of Pharmacy, Chinese PLA General Hospital, Beijing, China; Beijing Institute of Pharmacology and Toxicology, Beijing, China.
Abstract
Peramivir is a novel influenza neuraminidase inhibitor used for anti-influenza. In this article, a novel method was developed to determine peramivir in dog plasma using a derivatization treatment step to increase the retention time and enhance the signal intensity. The sample preparation consisted of a protein precipitation extraction followed by derivatization with 10M hydrochloric acid-methanol (10:90, v/v) and determined by liquid chromatography coupled with tandem mass spectrometry. The selected reaction monitoring mode of the positive ion was performed and the precursor to the product ion transitions of m/z 343→284 and m/z 299→152 were used to measure the derivative of peramivir and Ro 64-0802 (internal standard, an active metabolite of oseltamivir). The chromatographic separation was achieved using a ZORBAX RX-C8 (2.0mm?150mm?5μm) analytical column with an isocratic mobile phase composed of acetonitrile-water-formic acid (30:70:0.1, v/v/v, 0.2mL/min). The method was linear over a concentration range of 0.25-250ng/mL. The average intra-day/inter-day precision values were 4.04-8.17% and 3.02-7.08%, respectively, while the average accuracy value was 93.99-106.48%. This method has been successfully applied to the preclinical dog research of peramivir following intragastric administration.
Copyright ? 2013 Elsevier B.V. All rights reserved.
KEYWORDS:
Derivatization, Dog plasma, Influenza virus neuraminidase inhibitor, Liquid chromatography?tandem mass spectrometric method (LC?MS/MS), Peramivir
PMID:
24280376
[PubMed - as supplied by publisher]
Quantification of peramivir in dog plasma by liquid chromatography/tandem mass spectrometry employing precolumn derivatization.
Li X, Li Y, Wang J, Wang L, Zhong W, Ruan J, Zhang Z.
Source
Department of Pharmacy, Chinese PLA General Hospital, Beijing, China; Beijing Institute of Pharmacology and Toxicology, Beijing, China.
Abstract
Peramivir is a novel influenza neuraminidase inhibitor used for anti-influenza. In this article, a novel method was developed to determine peramivir in dog plasma using a derivatization treatment step to increase the retention time and enhance the signal intensity. The sample preparation consisted of a protein precipitation extraction followed by derivatization with 10M hydrochloric acid-methanol (10:90, v/v) and determined by liquid chromatography coupled with tandem mass spectrometry. The selected reaction monitoring mode of the positive ion was performed and the precursor to the product ion transitions of m/z 343→284 and m/z 299→152 were used to measure the derivative of peramivir and Ro 64-0802 (internal standard, an active metabolite of oseltamivir). The chromatographic separation was achieved using a ZORBAX RX-C8 (2.0mm?150mm?5μm) analytical column with an isocratic mobile phase composed of acetonitrile-water-formic acid (30:70:0.1, v/v/v, 0.2mL/min). The method was linear over a concentration range of 0.25-250ng/mL. The average intra-day/inter-day precision values were 4.04-8.17% and 3.02-7.08%, respectively, while the average accuracy value was 93.99-106.48%. This method has been successfully applied to the preclinical dog research of peramivir following intragastric administration.
Copyright ? 2013 Elsevier B.V. All rights reserved.
KEYWORDS:
Derivatization, Dog plasma, Influenza virus neuraminidase inhibitor, Liquid chromatography?tandem mass spectrometric method (LC?MS/MS), Peramivir
PMID:
24280376
[PubMed - as supplied by publisher]
