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Proteinase inhibitors from Streptomyces with antiviral activity
Original Paper
<table width="100%"><tbody><tr><td>Proteinase inhibitors from Streptomyces with antiviral activity
</td></tr><tr><td>Julia Serkedjieva, Dr.<sup> 1 *</sup>, Lidiya Angelova<sup> 1 2</sup>, Mimi Remichkova<sup> 1</sup>, Iskra Ivanova<sup> 2</sup></td></tr><tr><td><sup>1</sup>Institute of Microbiology, Bulgarian Academy of Sciences
<sup>2</sup>Department of Microbiology, Sofia University, Sofia, Bulgaria
</td></tr><tr><td>email: Julia Serkedjieva (jserkedjieva@microbio.bas.bg)</td></tr></tbody></table><sup>*</sup>Correspondence to Julia Serkedjieva, Tel.: (359 2) 979 31 85, Fax: (359 2) 70 01 09
<script language="JavaScript">setDOI("ADOI=10.1002/jobm.200510127")</script><table border="0" width="100%"><tbody><tr><td class="mainSectionHeader">Abstract</td></tr></tbody></table><table border="0" width="100%"><tbody><tr><td>An extensive screening study for the production of proteolytic inhibitors has been carried out on 75 Streptomyces strains. It was found that 18 of the strains and/or their variants (24%) produced proteinaceous substances, which belonged to the group of typical serine protease inhibitors. 23 samples were tested for inhibitory activity on the replication of influenza virus A/Germany/34, strain Rostock (H7N1) (A/Rostock) in chicken embryonic fibroblast (CEF) cells. Eleven of the tested samples (52.2%) significantly inhibited viral growth. Further the specific inhibitory effect on the replication of influenza virus A/Aichi/2/68 (H3N2) (A/Aichi) in Madin-Darby canine kidney (MDCK) cells and on the growth of herpes simplex virus type 1, strain DA (HSV-1) in Madin-Darby bovine kidney (MDBK) cells was tested. Nine samples significantly inhibited A/Aichi and four - HSV-1. The most effective inhibitors, produced by Streptomyces sp. 225b (SS 225b) and Streptomyces chromofuscus 34-1 (SS 34-1) protected mice from mortality in the experimental influenza A/Aichi virus infection. (? 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)</td></tr></tbody></table><hr align="left" size="1" width="25%">Received: 6 December 2005; Accepted: 28 January 2006<table border="0" width="100%"><tbody><tr><td class="mainSectionHeader">Digital Object Identifier (DOI)</td></tr></tbody></table>
10.1002/jobm.200510127 About DOI
<table width="100%"><tbody><tr><td>Proteinase inhibitors from Streptomyces with antiviral activity
</td></tr><tr><td>Julia Serkedjieva, Dr.<sup> 1 *</sup>, Lidiya Angelova<sup> 1 2</sup>, Mimi Remichkova<sup> 1</sup>, Iskra Ivanova<sup> 2</sup></td></tr><tr><td><sup>1</sup>Institute of Microbiology, Bulgarian Academy of Sciences
<sup>2</sup>Department of Microbiology, Sofia University, Sofia, Bulgaria
</td></tr><tr><td>email: Julia Serkedjieva (jserkedjieva@microbio.bas.bg)</td></tr></tbody></table><sup>*</sup>Correspondence to Julia Serkedjieva, Tel.: (359 2) 979 31 85, Fax: (359 2) 70 01 09
<script language="JavaScript">setDOI("ADOI=10.1002/jobm.200510127")</script><table border="0" width="100%"><tbody><tr><td class="mainSectionHeader">Abstract</td></tr></tbody></table><table border="0" width="100%"><tbody><tr><td>An extensive screening study for the production of proteolytic inhibitors has been carried out on 75 Streptomyces strains. It was found that 18 of the strains and/or their variants (24%) produced proteinaceous substances, which belonged to the group of typical serine protease inhibitors. 23 samples were tested for inhibitory activity on the replication of influenza virus A/Germany/34, strain Rostock (H7N1) (A/Rostock) in chicken embryonic fibroblast (CEF) cells. Eleven of the tested samples (52.2%) significantly inhibited viral growth. Further the specific inhibitory effect on the replication of influenza virus A/Aichi/2/68 (H3N2) (A/Aichi) in Madin-Darby canine kidney (MDCK) cells and on the growth of herpes simplex virus type 1, strain DA (HSV-1) in Madin-Darby bovine kidney (MDBK) cells was tested. Nine samples significantly inhibited A/Aichi and four - HSV-1. The most effective inhibitors, produced by Streptomyces sp. 225b (SS 225b) and Streptomyces chromofuscus 34-1 (SS 34-1) protected mice from mortality in the experimental influenza A/Aichi virus infection. (? 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)</td></tr></tbody></table><hr align="left" size="1" width="25%">Received: 6 December 2005; Accepted: 28 January 2006<table border="0" width="100%"><tbody><tr><td class="mainSectionHeader">Digital Object Identifier (DOI)</td></tr></tbody></table>
10.1002/jobm.200510127 About DOI
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